Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,687	T→C	T302A (ACT→GCT)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,687	0	T	C	100.0%	229.5 / NA	65	T302A (ACT→GCT)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/38);  total (27/38)

GTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTG  >  NC_000913/1541550‑1541816                                                                                                                                          |                                                                                                                                      gtcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATGACATGGCGCATGGCTACCGCCAGGGCGCTATCAgcgc                                                                                                                                     >  1:498126/1‑140 (MQ=255) gtcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAgcgc                                                                                                                                     >  1:31758/1‑140 (MQ=255)  tcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGcc                                                                                                                                    <  1:133000/140‑1 (MQ=255)  tcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGcc                                                                                                                                    >  1:1582895/1‑140 (MQ=255)       aGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTTAAATCACATGGCCCTTCGCCATCGCGAGGGCTCTATCAGAGCCTTGtt                                                                                                                               >  2:922773/1‑140 (MQ=255)       aGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGtt                                                                                                                               >  1:275685/1‑140 (MQ=255)          cTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCg                                                                                                                            <  2:942549/140‑1 (MQ=255)          cTCCAGCATTACCAGCACCGGCATGTCGCTGCAGCCGCGTCAGTAGTTCATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCACCGCCAGGGCGCTATCAGCGCCTTGTTTCg                                                                                                                            <  2:498126/140‑1 (MQ=255)           tCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCgg                                                                                                                           >  2:1217154/1‑140 (MQ=255)              aGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTgc                                                                                                                        <  2:1819722/140‑1 (MQ=255)               gCATTACCAGCATCGGCGTGTCTCTGTAGCGACGGCAGTAGTTGATAAGGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTgcc                                                                                                                       <  2:775222/140‑1 (MQ=255)                   tACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGTCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTgccagcc                                                                                                                   <  1:1808127/140‑1 (MQ=255)                   tACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTgccagcc                                                                                                                   >  1:1526714/1‑140 (MQ=255)                     ccAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCAc                                                                                                                 >  2:1297988/1‑140 (MQ=255)                      cAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACt                                                                                                                <  2:31758/140‑1 (MQ=255)                           tCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCg                                                                                                           >  2:1108496/1‑140 (MQ=255)                           tCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCg                                                                                                           <  1:1108496/140‑1 (MQ=255)                            cGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGc                                                                                                          <  2:1526714/140‑1 (MQ=255)                            cGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGc                                                                                                          >  1:1482228/1‑140 (MQ=255)                            cGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGc                                                                                                          >  2:330843/1‑140 (MQ=255)                            cGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGc                                                                                                          <  2:275685/140‑1 (MQ=255)                              gCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGcac                                                                                                        >  2:230622/1‑140 (MQ=255)                              gCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAAATCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGGCGcac                                                                                                        >  2:1575511/1‑140 (MQ=255)                                   tCGCTGTAGCGGCGGCAGGAGGTGAAAAAGTAGTCGCTGGGATTATCGTGATGGAACTCTTTTAAAATCACATGGCCCCTTTCCATCGCCAGGGGGCTATCAGCGCCTTGTTTTGGGGGCCGCCACCGGGCGCACAAttt                                                                                                   >  2:1115585/1‑140 (MQ=255)                                      cTGTAGCGTCGGCAGTAGTTGACAAAGTAGTCGGTCGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTTGTCGCACAATTTGGc                                                                                                <  2:909168/140‑1 (MQ=255)                                      cTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGc                                                                                                <  2:114581/140‑1 (MQ=255)                                      cTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGc                                                                                                <  1:230622/140‑1 (MQ=255)                                      cTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGc                                                                                                <  1:1115585/140‑1 (MQ=255)                                      cTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGc                                                                                                <  1:332874/140‑1 (MQ=255)                                        gTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCa                                                                                              <  2:308863/140‑1 (MQ=255)                                             ggcggcAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCa                                                                                         >  1:1344891/1‑140 (MQ=255)                                              gcggcAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATAGCCAGGGCGCTATCACCGCCTTGTATCGGGGCGAGCCACTGTTCGCACAATTTGGCCACTTCag                                                                                        >  2:650204/1‑140 (MQ=255)                                                ggcAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCACACAATTTGGCCACTTCaga‑‑‑‑g                                                                                  <  1:1195484/140‑1 (MQ=255)                                                 gcAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCACGGCGCTATCAGCGGCTTGTTTCAGTGGCTGTGACTGGGCGCACAAATTGGCCACTTCAGa‑‑‑‑gt                                                                                 >  2:868631/1‑140 (MQ=255)                                                   agtagtTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGc                                                                                                >  2:1899448/1‑127 (MQ=255)                                                   agtagtTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGc                                                                                                <  1:1899448/127‑1 (MQ=255)                                                   agtagtTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGa‑‑‑‑gtag                                                                               <  2:1582895/140‑1 (MQ=255)                                                                 tagtCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGc                                                                 >  1:1407036/1‑140 (MQ=255)                                                                 tagtCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGc                                                                 <  2:1344891/140‑1 (MQ=255)                                                                   gtCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGa                                                               <  1:1193720/140‑1 (MQ=255)                                                                   gtCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGa                                                               <  1:1217154/140‑1 (MQ=255)                                                                    tCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGAt                                                              >  2:1793786/1‑140 (MQ=255)                                                                    tCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGAt                                                              >  1:1089659/1‑140 (MQ=255)                                                                     cGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACACTTTGGCCACTTAAGA‑‑‑‑GTAGTCCGGGGTAATGGCGATg                                                             >  1:720227/1‑140 (MQ=255)                                                                     cGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATg                                                             >  1:1417724/1‑140 (MQ=255)                                                                     cGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATg                                                             >  1:326882/1‑140 (MQ=255)                                                                      gCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATgg                                                            >  1:1834837/1‑140 (MQ=255)                                                                        tGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGtt                                                          <  1:322975/140‑1 (MQ=255)                                                                         gggATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGttt                                                         >  2:1452506/1‑140 (MQ=255)                                                                         gggATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGttt                                                         >  1:1584341/1‑140 (MQ=255)                                                                            aTTATCGAGATGAAACTCTTTTAAAATCACATGTCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAg                                                      <  1:274018/140‑1 (MQ=255)                                                                             ttATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGt                                                     >  2:1002224/1‑140 (MQ=255)                                                                               aTCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGc                                                   <  1:1002224/140‑1 (MQ=255)                                                                                   agaTGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCttt                                               <  1:1452506/140‑1 (MQ=255)                                                                                    gaTGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTg                                              <  2:1417724/140‑1 (MQ=255)                                                                                    gaTGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTg                                              <  2:326882/140‑1 (MQ=255)                                                                                     aTGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGa                                                               <  1:288573/122‑1 (MQ=255)                                                                                     aTGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGa                                                               >  2:288573/1‑122 (MQ=255)                                                                                      tGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTa                                            <  2:9306/140‑1 (MQ=255)                                                                                       gAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAg                                           <  2:14659/140‑1 (MQ=255)                                                                                         aaCTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCg                                         <  2:1584341/140‑1 (MQ=255)                                                                                            tctTTTAAAATCACATGGCCCACTGCCATCGACAGCGCGATATCAGCGTCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTAc                                      <  2:716184/140‑1 (MQ=255)                                                                                              ttttAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACtt                                    <  1:330843/140‑1 (MQ=255)                                                                                              ttttAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACtt                                    <  1:1297988/140‑1 (MQ=255)                                                                                              ttttAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGCTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTCTGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGt                                        >  1:1581806/1‑140 (MQ=255)                                                                                                      tCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGCCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAaag                            >  1:1756866/1‑140 (MQ=255)                                                                                                         caTGGCCCATTGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAaagaag                         <  2:1601010/140‑1 (MQ=255)                                                                                                                   tGCCATCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCg               <  1:1575511/140‑1 (MQ=255)                                                                                                                        tCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTc                 <  1:1848237/133‑1 (MQ=255)                                                                                                                        tCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTc                 >  2:1848237/1‑133 (MQ=255)                                                                                                                        tCGCCAGGGCGCTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTGTTAGTGCCATTGGAGCATACTTCGGTAAAGAAGTGGGCGTCCGGCGTa          >  2:510831/1‑140 (MQ=255)                                                                                                                                gcgcTATCAGCGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTGCTTCGGTCAAGAAGGGGGCGTCCGGCGTACGTGTCTg  >  1:604409/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      GTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGA‑‑‑‑GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTG  >  NC_000913/1541550‑1541816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑