Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,554,860	G→A	D270D (GAC→GAT)	maeA ←	malate dehydrogenase, NAD‑requiring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,554,860	0	G	A	100.0%	294.9 / NA	84	D270D (GAC→GAT)	maeA	malate dehydrogenase, NAD‑requiring
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/43);  total (41/43)

TGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATT  >  NC_000913/1554727‑1554996                                                                                                                                      |                                                                                                                                         tgatCATTTCGGCAATGTCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGAGCCGACTATTAGCGGCGCAGTGCCCTGAATATCATCg                                                                                                                                    >  1:523750/1‑140 (MQ=255)  gatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGt                                                                                                                                   >  1:1014118/1‑140 (MQ=255)  gatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGt                                                                                                                                   >  2:371326/1‑140 (MQ=255)  gatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGt                                                                                                                                   >  2:1019326/1‑140 (MQ=255)  gatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTCTGCCGACGGTTACCGCTGCAGGGCCCTGAATATCATCGt                                                                                                                                   >  2:657331/1‑140 (MQ=255)       tttCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTaaaa                                                                                                                              <  2:1432581/140‑1 (MQ=255)           ggCAATGCCGCATCCCGCTGACTCTGCGCCAAGGACGACTTTTTTTTTCTCGCTTAACTGACCACCGGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAAc                                                                                                                          <  2:750254/140‑1 (MQ=255)            gCAATGCCGCATCCCGCTGAACCTGCGCCAACGAATACGCTTTTTTTCCCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACa                                                                                                                         <  2:539146/140‑1 (MQ=255)               aTGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTACCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAAt                                                                                                                      >  1:57646/1‑140 (MQ=255)                  tcGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTaa                                                                                                                                <  2:67923/126‑1 (MQ=255)                  tcGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTaa                                                                                                                                >  1:67923/2‑127 (MQ=255)                  ccGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTc                                                                                                                   >  2:1647077/1‑140 (MQ=255)                   cGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCa                                                                                                                  >  2:1860947/1‑140 (MQ=255)                    gCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCAt                                                                                                                 <  1:371326/140‑1 (MQ=255)                       tCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGc                                                                                                              >  1:1087454/1‑140 (MQ=255)                         ccGCTGAACCTGCGCCAAGGAATACAATTCTTTTCTCCCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGa                                                                                                            <  1:541440/140‑1 (MQ=255)                         ccGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGa                                                                                                            <  2:377241/140‑1 (MQ=255)                          cGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGAt                                                                                                           <  2:57646/140‑1 (MQ=255)                          cGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGAt                                                                                                           <  2:1275932/140‑1 (MQ=255)                          cGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATAATCGTTAAAAGAACAAATTTCATTGCGAt                                                                                                           >  1:827858/1‑140 (MQ=255)                          cGCTGAACCTGCGCCAACGAAGTCGATTTTTTTCTCGCTGAACTGACCCCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGAt                                                                                                           <  2:751032/140‑1 (MQ=255)                          cGCTGAACCTGCGCCAACGAAGACGATTTTTCTCTGGCTAAACTGACCCCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGAt                                                                                                           <  2:523750/140‑1 (MQ=255)                           gCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATa                                                                                                          >  2:963745/1‑140 (MQ=255)                            cTGACCCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAg                                                                                                         <  2:22915/140‑1 (MQ=255)                            cTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAg                                                                                                         >  1:984346/1‑140 (MQ=255)                            cTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCAGCAGTGCCGTGAATATGATCGTTAAGAGAATAAATTTCATTGCGATAg                                                                                                         >  2:541440/1‑140 (MQ=255)                                    gcgcCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAg                                                                                                 >  2:1748907/1‑140 (MQ=255)                                         aaGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACg                                                                                            >  2:1468673/1‑140 (MQ=255)                                            gAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCa                                                                                         <  1:1488987/140‑1 (MQ=255)                                                      tttttCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCAttttt                                                                               <  1:327461/140‑1 (MQ=255)                                                       ttttCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCAtttttt                                                                              >  2:375583/1‑140 (MQ=255)                                                             cGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCa                                                                        <  1:1354409/140‑1 (MQ=255)                                                              gCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCaa                                                                       <  2:984346/140‑1 (MQ=255)                                                              gCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCaa                                                                       >  2:1721742/1‑140 (MQ=255)                                                              gCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCaa                                                                       <  1:972205/140‑1 (MQ=255)                                                                    cTGACCACCTGCCGCGCGGCTTTCTGCGATCTGTGGGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCtt                                                                 <  2:827858/140‑1 (MQ=255)                                                                      gACCACCTGCGGCGCGGTTTGCGGCGATCGTGGTGGCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCa                                                               <  2:737409/140‑1 (MQ=255)                                                                      gACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCa                                                               >  2:1400647/1‑140 (MQ=255)                                                                      gACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCa                                                               <  1:1400647/140‑1 (MQ=255)                                                                      gACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCa                                                               <  1:1468673/140‑1 (MQ=255)                                                                       accaccTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGCCATAGCATTTTTTGGAGCAAAGTCTTCaa                                                              >  1:737409/1‑140 (MQ=255)                                                                        ccaccTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCaaa                                                             >  1:1848280/1‑140 (MQ=255)                                                                        ccaccTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCaaa                                                             >  1:376429/1‑140 (MQ=255)                                                                        ccaccTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCaaa                                                             >  1:1122201/1‑140 (MQ=255)                                                                        ccaccTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTAACGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGGATCTCACTTTTTTCAGCAAAGTCTTCaaa                                                             >  1:870623/1‑140 (MQ=255)                                                                          accTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACt                                                           >  1:1524653/1‑140 (MQ=255)                                                                            cTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGc                                                         >  1:252298/1‑140 (MQ=255)                                                                               ccGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAAc                                                      >  1:1196369/1‑140 (MQ=255)                                                                                 gcgcgGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAg                                                    <  2:1812182/140‑1 (MQ=255)                                                                                 gcgcgGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAg                                                    >  1:1065155/1‑140 (MQ=255)                                                                                 gcgcgGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAg                                                    <  2:1524653/140‑1 (MQ=255)                                                                                    cgGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTGTGAGCAAAGTCTTGAAACTGCAACAGCAc                                                 >  1:787142/1‑140 (MQ=255)                                                                                           ctgcGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGc                                          <  1:1647077/140‑1 (MQ=255)                                                                                              cGATCAGTGTGCCGACTGTTACAGCAGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAg                                       <  1:792167/140‑1 (MQ=255)                                                                                                aTCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCg                                     <  1:1660825/140‑1 (MQ=255)                                                                                                       tgCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTc                              >  2:442711/1‑140 (MQ=255)                                                                                                        gCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTca                             <  1:442711/140‑1 (MQ=255)                                                                                                        gCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTca                             <  1:963745/140‑1 (MQ=255)                                                                                                        gCCGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTca                             <  1:1748907/140‑1 (MQ=255)                                                                                                         ccGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTcac                            >  2:937156/1‑140 (MQ=255)                                                                                                          cGACTGTTACCGCCGCATTGCCGTGAATATCATCGTTAACAGAACAAAATTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTcaca                           <  2:787142/140‑1 (MQ=255)                                                                                                          cGACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATTGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGTCAGCGTTGTTTcaca                           >  1:1888131/1‑140 (MQ=255)                                                                                                           gACTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAg                          <  1:1860947/140‑1 (MQ=255)                                                                                                            gcTGTTACCGCCGCAGTCCCCTTAATATCATTGTTCAAATAACAAATTCCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGc                         <  2:535118/139‑1 (MQ=255)                                                                                                            aCTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGc                         >  1:218335/1‑140 (MQ=255)                                                                                                             cTGTTACCGCCGCAGTGGCCTGGATATCATCATTATAAGGACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGcc                        <  2:658566/140‑1 (MQ=255)                                                                                                             cTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCATGTATGGCCAGCGTTGTTTCACAGcc                        >  1:752463/1‑140 (MQ=255)                                                                                                             cTGTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGcc                        >  1:294113/1‑140 (MQ=255)                                                                                                             cTGTTACCACCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCAAGTCTGGCCAACGTTGTTTCACAGcc                        >  1:726723/1‑140 (MQ=255)                                                                                                               gTTACCGCCGCAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTg                      >  1:174493/1‑140 (MQ=255)                                                                                                                      ccgcAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGcc                        <  2:234369/131‑1 (MQ=255)                                                                                                                      ccgcAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGcc                        >  1:234369/1‑131 (MQ=255)                                                                                                                       cgcAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAAtt              >  1:1835958/1‑140 (MQ=255)                                                                                                                        gcAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCGCGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATtc             >  1:658566/1‑140 (MQ=255)                                                                                                                        gcAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGGCAGGGTAGTTTCATAGCCTGGATAAATtc             >  1:535118/1‑140 (MQ=255)                                                                                                                         cAGTGCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATtca            <  1:1721742/140‑1 (MQ=255)                                                                                                                             gCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCaa        <  1:1019326/140‑1 (MQ=255)                                                                                                                             gCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCaa        <  2:1353241/140‑1 (MQ=255)                                                                                                                             gCCCTGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCaa        <  1:1308758/140‑1 (MQ=255)                                                                                                                                 tGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGaa    <  2:218335/140‑1 (MQ=255)                                                                                                                                 tGAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGaa    <  1:937156/140‑1 (MQ=255)                                                                                                                                  gAATATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAAt   <  2:376429/140‑1 (MQ=255)                                                                                                                                  gAATATCAACGTTAAACGAACAAATTTCATTGCCATAGCGGTTAAGTACCGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAAt   <  2:870623/140‑1 (MQ=255)                                                                                                                                   aaTATCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAAtt  <  2:174493/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATT  >  NC_000913/1554727‑1554996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑