BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	135,603	A→G	S288P (TCC→CCC)	speE ←	spermidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	135,603	0	A	G	100.0%	421.0 / NA	115	S288P (TCC→CCC)	speE	spermidine synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (55/60); total (55/60)

Only 100 of 117 total aligned reads displayed.

TATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCG  >  NC_000913/135468‑135738

tataAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGggcggc                                                                                                                                     >  2:141050/1‑140 (MQ=255)

     gCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGa                                                                                                                                <  1:304814/140‑1 (MQ=255)

     cagatatAACCGTCGCGCTCTTAGGTAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGa                                                                                                                                <  1:804257/137‑1 (MQ=255)

         tataACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGcc                                                                                                                            >  1:198189/1‑140 (MQ=255)

         tataACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGcc                                                                                                                            >  2:354649/1‑140 (MQ=255)

         tataACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGcc                                                                                                                            >  2:46676/1‑140 (MQ=255)

         tataACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGcc                                                                                                                            >  2:1774668/1‑140 (MQ=255)

         tataACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGcc                                                                                                                            >  2:1803471/1‑140 (MQ=255)

         tataACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTCTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGcc                                                                                                                            >  2:71096/1‑140 (MQ=255)

          ataACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCa                                                                                                                           >  2:1688012/1‑140 (MQ=255)

          ataACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCa                                                                                                                           >  1:1413056/1‑140 (MQ=255)

          ataACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCa                                                                                                                           >  1:1701544/1‑140 (MQ=255)

            aaCCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGt                                                                                                                         <  1:1068897/140‑1 (MQ=255)

              ccGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCATTTTTTTCAATTTCTCACCTTCTCCTAAGGGCCGCTGTGAAGCCAGTGc                                                                                                                       >  2:852564/1‑140 (MQ=255)

              ccGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCg                                                                                                                                       >  1:1541600/1‑124 (MQ=255)

              ccGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTCTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGc                                                                                                                       <  1:71096/140‑1 (MQ=255)

               cGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCg                                                                                                                      >  2:105196/1‑140 (MQ=255)

                gTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGt                                                                                                                     >  1:1492025/1‑140 (MQ=255)

                gTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGt                                                                                                                     <  1:1688012/140‑1 (MQ=255)

                 tCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGt                                                                                                                         >  1:344758/1‑135 (MQ=255)

                 tCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGt                                                                                                                         <  2:344758/135‑1 (MQ=255)

                 tCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTc                                                                                                                    >  1:269605/1‑140 (MQ=255)

                 tCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCATATCTTCGCCTGAGAGCGGCTGTGAAGCCAGTGCGTc                                                                                                                    >  1:503810/1‑140 (MQ=255)

                   gcgcTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATGTTCGCCTTAGGGTGGCTGTGAAGCCAGTGCGTCtg                                                                                                                  >  2:804257/1‑139 (MQ=255)

                   gcgcTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCtt                                                                                                                  >  1:1634612/1‑140 (MQ=255)

                   gcgcTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCt                                                                                                                   >  1:1737942/1‑139 (MQ=255)

                     gcTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGc                                                                                                                <  2:1005026/140‑1 (MQ=255)

                     gcTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGc                                                                                                                >  2:194378/1‑140 (MQ=255)

                      ctctTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCa                                                                                                               <  2:1634612/140‑1 (MQ=255)

                        ctTCGGCAGTTTTGGCGTAGCAGATATCGTAAATATAAAGACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGa                                                                                                             <  2:867873/140‑1 (MQ=255)

                           cGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATAc                                                                                                          <  2:410855/140‑1 (MQ=255)

                           cGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATAc                                                                                                          <  2:1822842/140‑1 (MQ=255)

                                   ttGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTaa                                                                                                  >  2:1390812/1‑140 (MQ=255)

                                   ttGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTaa                                                                                                  >  1:1443660/1‑140 (MQ=255)

                                   ttGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTCAAGCCAGTGCGTCCTGCAGATACTGAGGTaa                                                                                                  >  2:662086/1‑140 (MQ=255)

                                           gCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAg                                                                                          <  1:1538387/140‑1 (MQ=255)

                                           gCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAg                                                                                          >  2:1446056/1‑140 (MQ=255)

                                           gCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGAGCGGCTGTAAAGGCAGAGCGTCTTGGAGATACTGAGGTAAGGCAAAAg                                                                                          >  2:491924/1‑140 (MQ=255)

                                           gCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCACGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAg                                                                                          >  2:443529/1‑140 (MQ=255)

                                              gATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTg                                                                                       <  2:1443660/140‑1 (MQ=255)

                                                   cGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTa                                                                                  <  1:1823101/140‑1 (MQ=255)

                                                   cGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTa                                                                                  <  1:951410/140‑1 (MQ=255)

                                                     tAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGGGTCTTGCGGATACTGATGTAAGGCAAAAGCTGCCGTATg                                                                                >  2:485113/1‑140 (MQ=255)

                                                       aaTACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTAAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGa                                                                              <  2:775110/140‑1 (MQ=255)

                                                          aCAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTg                                                                           <  2:122500/140‑1 (MQ=255)

                                                           cAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGc                                                                          <  2:1715340/140‑1 (MQ=255)

                                                           cAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGc                                                                          <  1:1774668/140‑1 (MQ=255)

                                                           cAAAAACTCAGACTTATGGTCAGATCATTGAAGCCATTCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGc                                                                          <  1:485113/140‑1 (MQ=255)

                                                            aaaaaCTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGc                                                                          >  1:346326/1‑139 (MQ=255)

                                                            aaaaaCTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGc                                                                          <  2:346326/139‑1 (MQ=255)

                                                             aaaaCTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAATGCAAAAGCTACCGTATGGATTGCCg                                                                        >  1:550405/1‑140 (MQ=255)

                                                             aaaaCTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCg                                                                        <  1:158889/140‑1 (MQ=255)

                                                              aaaCTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCt                                                                                        <  1:1400376/123‑1 (MQ=255)

                                                              aaaCTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCgg                                                                       <  2:983831/140‑1 (MQ=255)

                                                                aCTCAGACTTTTGGTGAGATTATTAAAGCCATGCAGTCTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGAt                                                                     <  1:852564/140‑1 (MQ=255)

                                                                aCTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGAt                                                                     <  1:1585278/140‑1 (MQ=255)

                                                                aCTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGAt                                                                     <  1:46676/140‑1 (MQ=255)

                                                                           tGGTCAGATTATTAATGCCATGAAGATTCTGTTTTTTTAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACgg                                                          <  1:800513/140‑1 (MQ=255)

                                                                           tGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACgg                                                          <  1:1194957/140‑1 (MQ=255)

                                                                           tGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACgg                                                          <  2:1492025/140‑1 (MQ=255)

                                                                           tGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACgg                                                          <  2:1643857/140‑1 (MQ=255)

                                                                           tGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAAGTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACgg                                                          >  2:1194957/1‑140 (MQ=255)

                                                                           tGGTCAGATTATTAAAGCCACGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACgg                                                          <  1:443529/140‑1 (MQ=255)

                                                                                aGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCAttt                                                     <  1:1803471/140‑1 (MQ=255)

                                                                                aGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCAttt                                                     <  1:194378/140‑1 (MQ=255)

                                                                                aGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCAttt                                                     <  2:319086/140‑1 (MQ=255)

                                                                                aGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCAttt                                                     <  1:354649/140‑1 (MQ=255)

                                                                                  attattAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCAttt                                                     >  2:262596/1‑138 (MQ=255)

                                                                                  attattAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCAttt                                                     <  1:262596/138‑1 (MQ=255)

                                                                                  attattAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCa                                                   <  1:141050/140‑1 (MQ=255)

                                                                                        aaaGCCATGCAGTTTCATTTTTCTCAATTTCATATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCag                                             <  2:550405/140‑1 (MQ=255)

                                                                                            ccATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGc                                         >  1:84891/1‑140 (MQ=255)

                                                                                            ccATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGc                                         >  2:1174150/1‑140 (MQ=255)

                                                                                              aTGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCga                                       >  2:1842322/1‑140 (MQ=255)

                                                                                              aTGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATGGCCGGATAGTAATATCGGCATATCAGGCCAGAGGCga                                       >  1:584248/1‑140 (MQ=255)

                                                                                              aTGCAGTTTCAGTTTTTGCAATTTCTTATCTCCTCTTTACGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCga                                       <  2:503810/140‑1 (MQ=255)

                                                                                                gCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCAttt                                                     <  2:1724043/124‑1 (MQ=255)

                                                                                                gCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCAttt                                                     >  1:1724043/1‑124 (MQ=255)

                                                                                                gCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCgaga                                     >  2:448437/1‑140 (MQ=255)

                                                                                                gCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCgaga                                     >  1:1082025/1‑140 (MQ=255)

                                                                                                gCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCgaga                                     >  1:212892/1‑140 (MQ=255)

                                                                                                       cagtttTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAAcgcg                              <  1:1842322/140‑1 (MQ=255)

                                                                                                        agtttTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACgcgc                             <  2:1413056/140‑1 (MQ=255)

                                                                                                         gtttTTTCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAAGAACTGCATTTCATGCCAAAGGCGAGAAAACGCGcc                            >  2:734753/1‑140 (MQ=255)

                                                                                                            ttttCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGa                         <  1:997227/140‑1 (MQ=255)

                                                                                                            ttttCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGa                         <  1:1390812/140‑1 (MQ=255)

                                                                                                            ttttCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGa                         <  2:212892/140‑1 (MQ=255)

                                                                                                              ttCAATTTCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGaat                       <  1:105196/140‑1 (MQ=255)

                                                                                                              ttCAATTTCTTATCTTCCCCTTAGGGCGGCTCTGATGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGaat                       <  2:584248/140‑1 (MQ=255)

                                                                                                                   tttCTTATCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAAttt                  <  1:1867684/140‑1 (MQ=255)

                                                                                                                         aTCTTCTCCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTg            >  1:972817/1‑140 (MQ=255)

                                                                                                                             tctcCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTgagag        >  1:14144/1‑140 (MQ=255)

                                                                                                                             cagccTTAGGGCGGCAGTGAAGCCACTGCGTCTTGCACATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTgagag        <  1:794130/137‑1 (MQ=255)

                                                                                                                              ctcCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTgagaga       <  2:1701544/140‑1 (MQ=255)

                                                                                                                               tcCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCGGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTgagag        >  2:1216451/1‑138 (MQ=255)

                                                                                                                               tcCTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTgagag        <  1:1216451/138‑1 (MQ=255)

                                                                                                                                 cTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAAtt                   <  2:1111670/125‑1 (MQ=255)

                                                                                                                                 cTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAAtt                   >  1:1111670/1‑125 (MQ=255)

                                                                                                                                 cTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATgg    >  2:1460071/1‑140 (MQ=255)

                                                                                                                                 cTTAGGGCGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATgg    >  2:936516/1‑140 (MQ=255)

TATAAGCAATATAACCGTCGCGCTCTTCGGCAGTTTTGGCGTAGCAGATATCGTAAATACAAAAACTCAGACTTTTGGTCAGATTATTAAAGCCATGCAGTTTCAGTTTTTTCAATTTCTTATCTTCTCCTTAGGACGGCTGTGAAGCCAGTGCGTCTTGCAGATACTGAGGTAAGGCAAAAGCTGCCGTATGGATTGCCGGATTGTAATAACGGCATTTCAGGCCAGAGGCGAGAAAACGCGCCTGAATAATTTCGGTTGAGAGATGGCG  >  NC_000913/135468‑135738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`