| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,717,884 | C→T | A42T (GCT→ACT) | pdxH ← | pyridoxal 5‑phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,717,884 | 0 | C | T | 98.4% | 202.3 / NA | 61 | A42T (GCT→ACT) | pdxH | pyridoxal 5‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (26/34); total (26/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAATTCGTCGTTATCAGACATGGTTTTC > NC_000913/1717748‑1718014 | cTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTg > 1:54677/1‑140 (MQ=255) gCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTgag > 2:1297579/1‑140 (MQ=255) gCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTgag > 2:1351137/1‑140 (MQ=255) gTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGcc < 1:1829695/140‑1 (MQ=255) ttGGTGTAAAACACCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCa < 1:1297579/140‑1 (MQ=255) ttGGTGTAAAACACCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCa < 1:1754290/140‑1 (MQ=255) tGGTGTAAAACACCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCaa < 2:224202/140‑1 (MQ=255) gtgtAAAACACCATGTCTTTTTTGTCGTAGTGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACg < 1:693837/140‑1 (MQ=255) tgtAAAACACCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTTCGCCAGTGTGGGTTCACAAGTTTGAGAGAGCCAACGt > 1:596302/1‑140 (MQ=255) acacCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCa > 1:71856/1‑130 (MQ=255) acacCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCa < 2:71856/130‑1 (MQ=255) acacCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCaaaa < 2:1584178/140‑1 (MQ=255) acacCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCaaaa < 1:319297/140‑1 (MQ=255) cacCATGCCTTTTTCGTGGTAATGTTTGAGTTAAACGATTCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCaaaaa < 2:596302/140‑1 (MQ=255) cATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTGTGGGAGAGCAAACGTTCAAAAAggg > 1:579402/1‑140 (MQ=255) cATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAggg > 1:1063349/1‑140 (MQ=255) tGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGtt > 1:1740943/1‑140 (MQ=255) ccTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTaa > 1:588828/1‑140 (MQ=255) tttCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGa < 1:1351137/140‑1 (MQ=255) tttCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGACTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGa < 2:588828/140‑1 (MQ=255) tcgtcgTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATc < 2:1063349/140‑1 (MQ=255) gtAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATcggcgg > 2:8644/1‑140 (MQ=255) gAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATcgc > 1:462409/1‑140 (MQ=255) gTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCgcgg < 1:388307/140‑1 (MQ=255) gTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCgcgg > 1:1619928/1‑140 (MQ=255) aaaaCGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCgcggc‑g > 1:1468839/1‑140 (MQ=255) aaaaCGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCgcggc‑g < 2:1740943/140‑1 (MQ=255) aaaCGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGGAGAACgcggg‑gg > 2:338809/1‑140 (MQ=255) aaaCGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCgcggc‑gg < 2:1619928/140‑1 (MQ=255) aTGCGCTGATAAGGCAGACCATATTGATCTACGGTAGAGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTaa < 1:794633/140‑1 (MQ=255) tGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGGAGATCGCGGC‑GGG‑GGAAc > 2:1210039/1‑140 (MQ=255) gcgcTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAAcc < 1:1567823/140‑1 (MQ=255) gATAAGGCTGACCATGTTCATCCACGGTAGCTACCTCCATCGCGGTAGCGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCt < 2:596007/140‑1 (MQ=255) gATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCt < 2:195674/140‑1 (MQ=255) aTAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCtt < 1:155276/140‑1 (MQ=255) aTAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCtt < 1:1657781/140‑1 (MQ=255) aTAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCt > 1:1147991/1‑139 (MQ=255) aTAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCt < 2:1147991/139‑1 (MQ=255) tAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCttt < 2:1631069/140‑1 (MQ=255) ccATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGGAGATCGCGGCGGGG‑GGAACCCCCCTTTgtgggttt > 2:1115680/1‑133 (MQ=255) ccATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGGAGAACGCGGCGGGG‑GGAACCCCCCTTTgtggattt > 1:1803620/1‑133 (MQ=255) ccATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGGAGAACGCGGC‑GGC‑GGAAACCGCCCTTTGTGTGttt > 2:155276/1‑139 (MQ=255) cATGTTCATCCACGGTAGCGACTACTATCGCGGTAGGTTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCa < 1:591523/140‑1 (MQ=255) cATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCa < 1:1184559/140‑1 (MQ=255) tCATCCACGGTAGCGACGACCATCGCGGTAGGGTCCGCCCGTTTGGCTGCATAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCACGgcg < 2:579402/140‑1 (MQ=255) tCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCACGgcg < 2:462409/140‑1 (MQ=255) tCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCACGgcg < 2:54677/140‑1 (MQ=255) tCCACGGTAGCTACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACTCCACCTTGGGGTATTTACCGCGCAg > 1:827166/1‑140 (MQ=255) tCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGG‑GGG‑GGAACCCCCCTTTGGTGTATTTACGgcgcgg > 1:350556/1‑138 (MQ=255) tCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCACGGCGCAg < 1:1848477/140‑1 (MQ=255) ggTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTAACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGGAGGTCGGGGC‑GGC‑GGAACCCCCCCTCGGGGGATTCACTgcgccgggacg > 2:1778379/1‑133 (MQ=255) cGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGGAGATCGCGGG‑GGG‑GGGACCCGCCCTTTGTGTATTTACGGCGCAGAAGCGCGGtt > 2:136716/1‑140 (MQ=255) accaccATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCACGGCACAGATGCGCGATTtg < 1:1206600/140‑1 (MQ=255) ggTAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAATtcgt < 1:1210039/140‑1 (MQ=255) tAGGGTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAATtcgtcg < 1:8644/140‑1 (MQ=255) gggTCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGGAGAACGCGGG‑GGCGGAAACCCCCCTTTGG‑GGGTTTTCGGCGCCGAAGCGCGGGTTGGTTCAATtcgtcggt > 2:1443594/1‑138 (MQ=255) gacagcGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAATTCGTCGtt < 1:121730/135‑1 (MQ=255) tCCGCCAGTTTGGCTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGGAGATCGCGGG‑GGCGGGAACCCCCCTCTGG‑GTATTTACGACGCGGAAGGGCGTGTTGTTGCAAAtcctcggtttt > 2:773279/1‑135 (MQ=255) tCCGCCAGTTTGGCTCCACAAGCCTTAGAAAGCCAACATTCAAAAAGGGTTGATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAATTCGTCGTTATc < 1:899185/140‑1 (MQ=255) gCCAGTTTGGCTTAACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAACTCGTCGTTATCAGa < 1:1778379/140‑1 (MQ=255) cTTCACAAGTCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGGAGATCGCGGG‑GGC‑GGAAACCCCCCTTTGTGGATTTACGGCGCAGATGCGCGGGTTTCTGCCATTCGTCGTTATCAGACATgggtgtt > 2:121730/1‑135 (MQ=255) | CTGCCGAGGTTGGTGTAAAACACCATGCCTTTTTCGTCGTAATGTTTGAGTAAAACGATGCGCTGATAAGGCTGACCATGTTCATCCACGGTAGCGACCACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGC‑GGC‑GTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAATTCGTCGTTATCAGACATGGTTTTC > NC_000913/1717748‑1718014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |