Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,728,630	C→T	A95V (GCG→GTG)	rnt →	RNase T

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,728,630	0	C	T	98.7%	225.1 / NA	74	A95V (GCG→GTG)	rnt	RNase T
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (38/35);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTATGATGGCCGCCGCAGAACGCGCCTCACTGAAAC  >  NC_000913/1728494‑1728764                                                                                                                                         |                                                                                                                                       ggctggctGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCt                                                                                                                                     >  1:1086433/1‑140 (MQ=255) ggctggctGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCt                                                                                                                                     >  2:1718494/1‑140 (MQ=255)  gctggctgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTg                                                                                                                                    >  1:1544424/1‑140 (MQ=255)  gctggctgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTg                                                                                                                                    >  2:1786543/1‑140 (MQ=255)        tgATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGaa                                                                                                                              <  2:1333295/140‑1 (MQ=255)           tGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAAtt                                                                                                                           >  2:67639/1‑140 (MQ=255)               ggACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTa                                                                                                                       <  1:1718494/140‑1 (MQ=255)                    ccacATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGtt                                                                                                                  >  1:1838307/1‑140 (MQ=255)                    ccacATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGtt                                                                                                                  <  1:1749681/140‑1 (MQ=255)                    ccacATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGtt                                                                                                                  <  1:1638281/140‑1 (MQ=255)                         ttacattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACg                                                                                                             <  2:1838307/140‑1 (MQ=255)                            cattTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTaa                                                                                                          <  1:428645/140‑1 (MQ=255)                             attTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTaaa                                                                                                         >  1:1195145/1‑140 (MQ=255)                               ttCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGTGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTaa                                                                                                          >  2:1144456/1‑137 (MQ=255)                               ttCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGTGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTaa                                                                                                          <  1:1144456/137‑1 (MQ=255)                               ttCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAgg                                                                                                       >  1:1717338/1‑140 (MQ=255)                               ttCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAgg                                                                                                       >  1:1768329/1‑140 (MQ=255)                                 ccACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTa                                                                                                     >  2:122821/1‑140 (MQ=255)                                 ccACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACCAGGTGCTACACGAAATCTTTACAGTTGTACGTAAAGGTa                                                                                                     >  2:558464/1‑140 (MQ=255)                                  cACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTAt                                                                                                    >  1:1449593/1‑140 (MQ=255)                                    cGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTa                                                                                                  >  2:1272852/1‑140 (MQ=255)                                    cGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTa                                                                                                  >  1:207288/1‑140 (MQ=255)                                    cGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGACCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTa                                                                                                  >  1:248980/1‑140 (MQ=255)                                         aaCCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAagcg                                                                                             >  1:276880/1‑140 (MQ=255)                                          aCCATTTGTCGGCGCAACTTTGCAACCAGAACCCCGCGCATTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAagcga                                                                                            <  2:591336/140‑1 (MQ=255)                                          aCCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAagcga                                                                                            <  2:1849007/140‑1 (MQ=255)                                          aCCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAagcga                                                                                            <  2:1086433/140‑1 (MQ=255)                                          aCCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAagcga                                                                                            <  1:1444817/140‑1 (MQ=255)                                          aCCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAagcga                                                                                            <  1:1440160/140‑1 (MQ=255)                                           ccATTTGTCGGCGCAAATTTGCAACCAGAAGGCCTAGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAagcgag                                                                                           <  2:761929/140‑1 (MQ=255)                                           ccATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGTCCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAagcgag                                                                                           <  2:1487719/140‑1 (MQ=255)                                           ccATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAagcgag                                                                                           <  2:1195145/140‑1 (MQ=255)                                           ccATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAagcgag                                                                                           <  2:1674518/140‑1 (MQ=255)                                            cATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAagcgagc                                                                                          <  1:1272852/140‑1 (MQ=255)                                                 gTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTg                                                                                     >  1:1052519/1‑140 (MQ=255)                                                 gTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTATTAAAGTTGTAGGTAAAGGTAATAAAGCGAGCGGCTg                                                                                     >  1:591336/1‑140 (MQ=255)                                                    ggCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTaa                                                                                  >  2:1682791/1‑140 (MQ=255)                                                      cgcAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAACGTTGTACATAATGGTATTAAATCGAGCGGCTGTAAcc                                                                                >  1:881015/1‑140 (MQ=255)                                                      cgcAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAAcc                                                                                >  2:1824097/1‑140 (MQ=255)                                                      cgcAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAAcc                                                                                >  1:1775413/1‑140 (MQ=255)                                                              tGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCAtt                                                                        <  1:1682791/140‑1 (MQ=255)                                                               gCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTa                                                                       <  2:1052519/140‑1 (MQ=255)                                                                     aGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTAtggtgg                                                                 <  2:1768329/140‑1 (MQ=255)                                                                       aaGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGgcg                                                               >  1:1150618/1‑140 (MQ=255)                                                                          cccTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGcac                                                            <  1:1824097/140‑1 (MQ=255)                                                                             tCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAAt                                                         >  2:1087685/1‑140 (MQ=255)                                                                              cGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGca                                                             >  1:102957/1‑135 (MQ=255)                                                                              cGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGca                                                             <  2:102957/135‑1 (MQ=255)                                                                              cGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATg                                                        >  1:408115/1‑140 (MQ=255)                                                                               gCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGATGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCACCCCATTATGGTGGCGCAAAATGc                                                       >  2:686323/1‑140 (MQ=255)                                                                                 cTTCAACGGCATTGACCCGAACGATGCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCa                                                     <  1:841096/140‑1 (MQ=255)                                                                                    cAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAAtt                                                  >  2:430555/1‑140 (MQ=255)                                                                                    cAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAAtt                                                  >  2:195392/1‑140 (MQ=255)                                                                                    cAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAAtt                                                  <  1:195392/140‑1 (MQ=255)                                                                                    cAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAAtt                                                  >  1:1270039/1‑140 (MQ=255)                                                                                         gCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTACCCGCGCCATTACGGCGGCGCAAAATGCCAATTTTGAt                                             >  1:632455/1‑140 (MQ=255)                                                                                           aTTGACCCGAACGATGCCGATCCCGTCGCCGTCAGCGTATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATca                                           <  1:574836/140‑1 (MQ=255)                                                                                           aTTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATca                                           <  2:1449593/140‑1 (MQ=255)                                                                                           aTTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATca                                           <  1:1786543/140‑1 (MQ=255)                                                                                           aTTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATca                                           <  2:1717338/140‑1 (MQ=255)                                                                                           aTTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATca                                           <  2:1775413/140‑1 (MQ=255)                                                                                               aCCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGGTGTAACCGCGCCCTTAGGGTGGCGCCCAATCCCAATTTTGATCACAGc                                       >  2:574836/1‑140 (MQ=255)                                                                                               aCCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCa                                                     >  2:1339918/1‑126 (MQ=255)                                                                                               aCCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCa                                                     <  1:1339918/126‑1 (MQ=255)                                                                                                cccGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCACCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCt                                      <  2:276880/140‑1 (MQ=255)                                                                                                    aaCGATCCCGATCGCGTCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGGGCCATTATAGTGGCGCACAATGCCAACTTTGATCACAGCTTTat                                  >  2:799495/1‑140 (MQ=255)                                                                                                    aaCGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTat                                  >  1:52522/1‑140 (MQ=255)                                                                                                     aCGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTatg                                 <  1:67639/140‑1 (MQ=255)                                                                                                          cccGAGCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTATGATGgc                            <  2:207288/140‑1 (MQ=255)                                                                                                           ccGATCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTATGATGgcc                           <  2:1270039/140‑1 (MQ=255)                                                                                                               tCGCGGCGCGGTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTATGATGGccgccg                       >  1:324081/1‑140 (MQ=255)                                                                                                                   ggcgcggTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTATGATGGCCGCCGCAGa                   >  1:359114/1‑140 (MQ=255)                                                                                                                     cgcggTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTATGATGGCCGCCGCAGAAc                 >  1:1571243/1‑140 (MQ=255)                                                                                                                     cgcggTCAGCGAATACGAGGCGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTATGATGGCCGCCGCAGAAc                 >  1:999838/1‑140 (MQ=255)                                                                                                                      gcggTCAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTATGATGGCCGCCGCAGAAcg                <  2:324081/140‑1 (MQ=255)                                                                                                                        ggTCAGCGCATGCGAGGCGCGGTACGAAATGGTAACAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGGGCCATTATGGTGGCGAACACTGCCAATTTTGATCACAGATTTATGATGGCCGCCGCAGAAcgcg              <  2:881015/140‑1 (MQ=255)                                                                                                                           cAGCGAATACGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTATGATGGCCGCCGCAGAACGCGCCt           <  2:1544424/140‑1 (MQ=255)                                                                                                                                    cGAGGTGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATATTGATCACGGCTATATATTGGCCGCCGCAGAGCGCGCCTCACTGAAAc  >  2:875092/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTATGATGGCCGCCGCAGAACGCGCCTCACTGAAAC  >  NC_000913/1728494‑1728764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑