Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	178,897	A→G	V86A (GTG→GCG)	mtn ←	5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	178,897	0	A	G	100.0%	270.3 / NA	73	V86A (GTG→GCG)	mtn	5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/40);  total (33/40)

GCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGT  >  NC_000913/178762‑179033                                                                                                                                        |                                                                                                                                         gCCTCAGCTGCAGCGATCAGTTTATCGTCAGCTTTTAAGCCTGCCGGAAAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCttt                                                                                                                                      <  2:894072/140‑1 (MQ=255)   cTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCa                                                                                                                                    >  2:246099/1‑140 (MQ=255)      aGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACg                                                                                                                                 >  1:1317201/1‑140 (MQ=255)      aGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCC‑‑GACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGtt                                                                                                                               <  2:1390578/140‑1 (MQ=255)       gCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGt                                                                                                                                <  2:1317201/140‑1 (MQ=255)           cAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGCTAACGTGCTTCGACCGGGACAACGAAATCGACCGCTTTCAACGTTGGt                                                                                                                            >  2:714599/1‑140 (MQ=255)              cGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGcc                                                                                                                         <  1:90873/140‑1 (MQ=255)                aTCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAg                                                                                                                       >  1:1175918/1‑140 (MQ=255)                        aTCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCgg                                                                                                               >  1:1820946/1‑140 (MQ=255)                        aTCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACCGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCgg                                                                                                               <  1:990190/140‑1 (MQ=255)                          cGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGTTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCa                                                                                                             >  1:1103457/1‑140 (MQ=255)                           gTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAg                                                                                                            <  2:1790770/140‑1 (MQ=255)                            tCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGa                                                                                                           >  2:453708/1‑140 (MQ=255)                             cAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGaa                                                                                                          >  2:943976/1‑140 (MQ=255)                               gCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGATAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAAcc                                                                                                        <  1:1844248/140‑1 (MQ=255)                               gCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCAc                                                                                                                  <  2:1583360/130‑1 (MQ=255)                               gCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCAc                                                                                                                  >  1:1583360/1‑130 (MQ=255)                               gCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAAcc                                                                                                        >  2:51668/1‑140 (MQ=255)                               gCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAAcc                                                                                                        >  2:1113575/1‑140 (MQ=255)                                cTTTAAAGCCTGCCGGGAAGCCGGGTAACTGGCGGTATTGATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCg                                                                                                       <  2:843467/140‑1 (MQ=255)                                 tttAAAGCCTGCCGGACCGCCTTGTAACTGACCGTGTTCCTAACCAAAAGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCgg                                                                                                      <  2:501829/140‑1 (MQ=255)                                 tttAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCgg                                                                                                      <  1:453708/140‑1 (MQ=255)                                  ttAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGgt                                                                                                     <  1:246099/140‑1 (MQ=255)                                  ttAAAGCCTGCCGGACAGCCTGCTAGCTGTCCGTATTTATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGgt                                                                                                     <  1:714599/140‑1 (MQ=255)                                      aGCCTGCCGGACAGCCTGGTAACTGTCCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTta                                                                                                 <  1:743132/140‑1 (MQ=255)                                       gCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTtaa                                                                                                <  1:51668/140‑1 (MQ=255)                                          tGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTtaataa                                                                                             >  2:1443985/1‑140 (MQ=255)                                          tGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTtaataa                                                                                             >  1:1053927/1‑140 (MQ=255)                                          tGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTtaataa                                                                                             >  1:298795/1‑140 (MQ=255)                                           gCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCGCGCTTTCAGCGTAGGTCCCAGGCCATCGGCAGAACCGGTGTTaataat                                                                                            >  2:517763/1‑140 (MQ=255)                                           gCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTaataat                                                                                            <  2:1053927/140‑1 (MQ=255)                                                aCAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACAt                                                                                       <  2:1175918/140‑1 (MQ=255)                                                      tGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCt                                                                                 >  2:1311709/1‑140 (MQ=255)                                                         tAACTGACCGTATTCATAACCAAATGCCGTGTCATCCGCTTCGTGATACCGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                                                              <  2:773770/140‑1 (MQ=255)                                                         tAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                                                              <  1:1113575/140‑1 (MQ=255)                                                         tAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                                                              <  2:1820946/140‑1 (MQ=255)                                                         tAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                                                              <  1:1458465/140‑1 (MQ=255)                                                            cTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAgt                                                                           <  2:298795/140‑1 (MQ=255)                                                              gACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                                                              <  2:1334628/135‑1 (MQ=255)                                                              gACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                                                              >  1:1334628/1‑135 (MQ=255)                                                              gACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAgtgt                                                                         >  2:1814821/1‑140 (MQ=255)                                                                 cGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                                                              <  2:1630711/132‑1 (MQ=255)                                                                 cGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGc                                                                              >  1:1630711/1‑132 (MQ=255)                                                                 cGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTcc                                                                      >  2:1230174/1‑140 (MQ=255)                                                                   tATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCaa                                                                    <  1:1747265/140‑1 (MQ=255)                                                                      tCATAACCAAATGCCGTTACATACGCGTCGTGATAACGTACTTCGTCCTAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAg                                                                 <  2:666268/140‑1 (MQ=255)                                                                      tCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGTTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAg                                                                 <  2:1103457/140‑1 (MQ=255)                                                                           aCCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAg                                                            >  1:952590/1‑140 (MQ=255)                                                                               aaTGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGc                                                        >  2:1414382/1‑140 (MQ=255)                                                                                 tGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCAc                                                      >  2:1171512/1‑140 (MQ=255)                                                                                      tGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATGATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAgc                                                 >  2:1290382/1‑140 (MQ=255)                                                                                      tGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCa                                                               <  1:1244725/126‑1 (MQ=255)                                                                                      tGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCa                                                               >  2:1244725/1‑126 (MQ=255)                                                                                      tGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAgc                                                 >  1:409934/1‑140 (MQ=255)                                                                                           tCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGcgccgc                                            <  1:1171512/140‑1 (MQ=255)                                                                                             cgcgTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAg                                          >  1:1889095/1‑140 (MQ=255)                                                                                              gcgTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGc                                         <  2:952590/140‑1 (MQ=255)                                                                                                gtcgtGATAACGTGCTTAGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGa                                       >  2:348964/1‑140 (MQ=255)                                                                                                  cgtGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACt                                     <  1:1443985/140‑1 (MQ=255)                                                                                                    tGATAACGTGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTCTTCGAACGGCAAAGTGACACCCCGCGCCGCTGCGACttt                                   >  2:845643/1‑140 (MQ=255)                                                                                                            tGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGc                           >  1:1446313/1‑140 (MQ=255)                                                                                                            tGCTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGc                           <  1:437955/140‑1 (MQ=255)                                                                                                              cTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGccc                         <  2:1446313/140‑1 (MQ=255)                                                                                                              cTTCGTCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGccc                         <  1:943976/140‑1 (MQ=255)                                                                                                                   tCCGAGACAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGAttt                    >  2:1432213/1‑140 (MQ=255)                                                                                                                      gagaCAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAg                 <  2:409934/140‑1 (MQ=255)                                                                                                                         aCAACGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAg              <  1:1311709/140‑1 (MQ=255)                                                                                                                           aaCGATATCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCCGCGCCGCCGCGACTTTTCCGATGCCCGATTTCAGAAgcg            >  2:667402/1‑140 (MQ=255)                                                                                                                            aCGATATCGCCCGCTTTCAACGGTGGTGCTAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAgcgc           <  1:667402/140‑1 (MQ=255)                                                                                                                                 atCGCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCt      <  1:1814821/140‑1 (MQ=255)                                                                                                                                  tCGCCCGCTCTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTc     >  1:1360062/1‑140 (MQ=255)                                                                                                                                    gCCCGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCgg   <  1:1230174/140‑1 (MQ=255)                                                                                                                                    gCCCGCTTTCAACGTGGGTGCCCGGTCACAGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCgg   <  1:845643/140‑1 (MQ=255)                                                                                                                                     cccGCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGTGACGTAATCGATGCCCGATTTTAGAAGCGCAACCTCGGt  >  2:897078/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         GCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGT  >  NC_000913/178762‑179033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑