Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,940,663	T→C	T325A (ACG→GCG)	mepM ←	peptidoglycan DD‑endopeptidase MepM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,940,663	0	T	C	100.0%	324.7 / NA	90	T325A (ACG→GCG)	mepM	peptidoglycan DD‑endopeptidase MepM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (49/41);  total (49/41)

ACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCGCCGTCGGGAATCGCAAGAATCCTTTCGCCAG  >  NC_000913/1940533‑1940798                                                                                                                                   |                                                                                                                                        aCCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGAAACCACCACTTCTCCGACACCCACTGAAAGTACTGGCGCACCCTGCgg                                                                                                                                >  2:636656/1‑140 (MQ=255)          ttGCGCAAGTGCATATACCGCGTGGTGTAGCTGCGCCCATGACGAATAACCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGaa                                                                                                                       <  2:786813/140‑1 (MQ=255)           tGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGaaa                                                                                                                      >  1:1249567/1‑140 (MQ=255)            gcgcAAGTGCATATACCGCGTGTTGTAGTCGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAAt                                                                                                                     <  1:615123/140‑1 (MQ=255)              gcAAGTGCATATAACGCGTGGTTTAACTGGGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCa                                                                                                                   <  1:711442/140‑1 (MQ=255)               cAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCCCTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTCCGGCATGGCGAAATCaa                                                                                                                  <  2:1128766/140‑1 (MQ=255)                            cgcgTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTgtgg                                                                                                     >  2:1793637/1‑140 (MQ=255)                            cgcgTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTgtgg                                                                                                     >  1:953838/1‑140 (MQ=255)                             gcgTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTgtggt                                                                                                    >  1:469829/1‑140 (MQ=255)                              cgTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCGCCCTGCGACATTGCCAAATCAGCACCTCTGTgtggtg                                                                                                   >  1:723462/1‑140 (MQ=255)                              cgTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTgtggtg                                                                                                   >  1:1052127/1‑140 (MQ=255)                              cgTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTgtggtg                                                                                                   >  1:1018900/1‑140 (MQ=255)                              cgTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTgtggtg                                                                                                   >  2:1170735/1‑140 (MQ=255)                                    gtAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGAGGCATGGGGAAATCAACACCTCTGTGTGGTGCAAcgc                                                                                             >  1:717057/1‑140 (MQ=255)                                             aCCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCa                                                                                    <  1:1793637/140‑1 (MQ=255)                                             aCCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCa                                                                                    >  1:1917928/1‑140 (MQ=255)                                             aCCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCa                                                                                    >  1:1796952/1‑140 (MQ=255)                                             aCCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCa                                                                                    >  1:1710981/1‑140 (MQ=255)                                             aCCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCa                                                                                    >  1:1050521/1‑140 (MQ=255)                                             aCCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGCGCAACGCGACCGGTCa                                                                                    >  2:856908/1‑140 (MQ=255)                                              ccATGACGAATAGCCCCATAATTACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCAc                                                                                   <  1:636656/140‑1 (MQ=255)                                              ccATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCAc                                                                                   <  2:1710981/140‑1 (MQ=255)                                              ccATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCAc                                                                                   <  2:1018900/140‑1 (MQ=255)                                              ccATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCAc                                                                                   <  2:1050521/140‑1 (MQ=255)                                              ccATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCAc                                                                                   <  2:1415361/140‑1 (MQ=255)                                              ccATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCAc                                                                                   <  2:1232800/140‑1 (MQ=255)                                                aTGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCg                                                                                 <  1:856908/140‑1 (MQ=255)                                                aTGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCg                                                                                 <  2:953838/140‑1 (MQ=255)                                                 tGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCgg                                                                                >  1:1099435/1‑140 (MQ=255)                                                   aCGAATAGCCACATAATTACCTACTGCGCCATTGCGTTTAGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGAt                                                                              <  2:612036/140‑1 (MQ=255)                                                           ccACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTacga                                                                      >  1:434457/1‑140 (MQ=255)                                                             acaTAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTacgacg                                                                    >  2:1303684/1‑140 (MQ=255)                                                              caTAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGAcgc                                                                   <  2:414273/140‑1 (MQ=255)                                                              caTAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGAcgc                                                                   <  2:1099435/140‑1 (MQ=255)                                                              caTAATAAACTGCTGCTGCACTTCGTTTGGCAACCGCCATTTCACCGTTACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGAcgc                                                                   <  2:910619/140‑1 (MQ=255)                                                               ataataACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTacgacg                                                                    <  2:1218964/138‑1 (MQ=255)                                                               ataataACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTacgacg                                                                    >  1:1218964/1‑138 (MQ=255)                                                                taataaCCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCgg                                                                 >  2:1696655/1‑140 (MQ=255)                                                                taataaCCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCgg                                                                 >  2:85805/1‑140 (MQ=255)                                                                  ataaCCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGt                                                               <  2:1722695/140‑1 (MQ=255)                                                                   taaCCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGGCCGGTCACCGGATAAGTACGACGCGGGtt                                                              >  1:534718/1‑140 (MQ=255)                                                                   taaCCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGtt                                                              >  1:265073/1‑140 (MQ=255)                                                                        tgctgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAAt                                                         >  1:171352/1‑140 (MQ=255)                                                                         gctgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTacgacg                                                                    >  1:1603474/1‑128 (MQ=255)                                                                         gctgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTacgacg                                                                    <  2:1603474/128‑1 (MQ=255)                                                                         gctgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAAtt                                                        >  1:1583205/1‑140 (MQ=255)                                                                         gctgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTATGGTGCAAGGCGACCGGTCACCGGAATAGTTCGACGCGGGTTAAAAtt                                                        >  2:592076/1‑140 (MQ=255)                                                                         gctgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTATGGTGCAAGGCGACCGATCACCGGATTAGTACGACGCGGGTTAAAAtt                                                        >  2:706820/1‑140 (MQ=255)                                                                          ctgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTa                                                       >  1:205021/1‑140 (MQ=255)                                                                          ctgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTa                                                       >  1:377292/1‑140 (MQ=255)                                                                          ctgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTa                                                       >  1:1318117/1‑140 (MQ=255)                                                                           tgcGCCACTGCGTTTGGCGACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTacgacg                                                                    >  2:1063427/1‑126 (MQ=255)                                                                           tgcGCCACTGCGTTTGGCGACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTacgacg                                                                    <  1:1063427/126‑1 (MQ=255)                                                                           tgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTacgacg                                                                    <  1:448167/126‑1 (MQ=255)                                                                           tgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTacgacg                                                                    >  2:448167/1‑126 (MQ=255)                                                                           tgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGtt                                                              <  2:97083/132‑1 (MQ=255)                                                                           tgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGtt                                                              >  1:97083/1‑132 (MQ=255)                                                                           tgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAg                                                      >  2:274904/1‑140 (MQ=255)                                                                           tgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAg                                                      >  1:1800574/1‑140 (MQ=255)                                                                           tgcGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAg                                                      >  1:457403/1‑140 (MQ=255)                                                                            gcgcCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGa                                                     <  2:1318117/140‑1 (MQ=255)                                                                            gcgcCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGa                                                     <  2:434457/140‑1 (MQ=255)                                                                            gcgcCACTGCGTTTGGCAACCACCAATTCACCGTCACTCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGa                                                     <  1:706820/140‑1 (MQ=255)                                                                             cgcCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGAc                                                    >  1:1802638/1‑140 (MQ=255)                                                                              gcCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACg                                                   <  2:377292/140‑1 (MQ=255)                                                                               ccACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACga                                                  >  2:243374/1‑140 (MQ=255)                                                                                cACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACgag                                                 <  2:265073/140‑1 (MQ=255)                                                                                 aCTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTAACCGGATTAGTACGCCGCGGGGTAAAATTAGACgaga                                                >  1:767737/1‑140 (MQ=255)                                                                                  cTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCGCCGGATTGGTACGACGCGGGTTAAAATTAGACGAGAt                                               >  1:811527/1‑140 (MQ=255)                                                                                   tGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATa                                              <  1:85805/140‑1 (MQ=255)                                                                                   tGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATa                                              <  2:171352/140‑1 (MQ=255)                                                                                     cGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCCCCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACg                                            <  2:1796952/140‑1 (MQ=255)                                                                                     cGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACg                                            >  2:354469/1‑140 (MQ=255)                                                                                        ttGGCAACCACCACTTCTCCGTCACCCACTGAAAGCACTCGCGCACCCGGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGaa                                         <  2:767737/140‑1 (MQ=255)                                                                                        ttGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGaa                                         <  1:1303684/140‑1 (MQ=255)                                                                                               ccaccaCTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACgg                                           <  2:1901685/131‑1 (MQ=255)                                                                                               ccaccaCTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACgg                                           >  1:1901685/1‑131 (MQ=255)                                                                                               ccaccaCTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTc                                  <  2:1249567/140‑1 (MQ=255)                                                                                                caccacTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCg                                 <  2:1482833/140‑1 (MQ=255)                                                                                                caccacTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCg                                 <  1:348577/140‑1 (MQ=255)                                                                                                         aCCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCGCCGTCGGGa                        >  1:1157679/1‑140 (MQ=255)                                                                                                           cGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCGCCGTCGGGAAt                      <  2:1802638/140‑1 (MQ=255)                                                                                                               aCCCACTGAAAGCACCGGCGCACCCCGCGACATGGCGTAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCGCCGTCGGGAATCGCa                  <  1:592076/140‑1 (MQ=255)                                                                                                                       aaaGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCGCCGTCGGGAATCGCAAGAATCCt          >  2:1158589/1‑140 (MQ=255)                                                                                                                       aaaGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCGCCGTCGGGAATCGCAAGAATCCt          <  1:1392436/140‑1 (MQ=255)                                                                                                                         aGCACTGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCGCCGTCGGGAATCGCAAGAATCCttt        >  2:1912673/1‑140 (MQ=255)                                                                                                                            aCTGGCGCACCCTGCCGCATGGAGAGATCAACACCTCGGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCGCCGTCGGGAATCGCAAGAATCCTTTCGc     <  1:628276/140‑1 (MQ=255)                                                                                                                              tGGCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCGCCGTCGGGAATCGCAAGAATCCTTTCGCCa   >  1:284973/1‑140 (MQ=255)                                                                                                                               ggCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGACCTGATTCACCGTCGGGAATCGTAAGAATCCTTTCGCCAg  >  2:628276/1‑140 (MQ=255)                                                                                                                               ggCGCACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACCGTTTCGCCGTCAGGCATCGCACGAATCCTTTCGCCAg  >  1:884737/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        ACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAGTACGACGCGGGTTAAAATTAGACGAGATACGGAACTGTTTCGCCGTCGGGAATCGCAAGAATCCTTTCGCCAG  >  NC_000913/1940533‑1940798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑