BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,095,848	T→C	W242R (TGG→CGG) L2P (CTG→CCG)	hisA → hisF →	1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase imidazole glycerol phosphate synthase subunit HisF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,095,848	0	T	C	99.1%	424.7 / NA	115	W242R (TGG→CGG) L2P (CTG→CCG)	hisA hisF	1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase imidazole glycerol phosphate synthase subunit HisF
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (59/55); total (59/56)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

GGTGGCATTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTG  >  NC_000913/2095712‑2095984

ggTGGCATTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGc                                                                                                                                       >  1:423383/1‑140 (MQ=255)

  tGGCATTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCaa                                                                                                                                     <  2:410969/140‑1 (MQ=255)

   ggCATTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCaaa                                                                                                                                    >  1:1118761/1‑140 (MQ=255)

   ggCATTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCaaa                                                                                                                                    <  1:1789150/140‑1 (MQ=255)

      aTTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACg                                                                                                                                 >  2:1260812/1‑140 (MQ=255)

        ttCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCa                                                                                                                               <  2:1118761/140‑1 (MQ=255)

          cAGTCCTCCGGCGGTATTGGCGCCATTGATGCTGTCGCGACCCTGCGTAGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATa                                                                                                                             <  2:580264/140‑1 (MQ=255)

          cAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATa                                                                                                                             <  2:1043608/140‑1 (MQ=255)

          cAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATa                                                                                                                             <  2:1216450/140‑1 (MQ=255)

          cAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATa                                                                                                                             <  2:948169/140‑1 (MQ=255)

                  cggcggTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCAGCGCATGCCGGCAAAACGCATAATCCCATg                                                                                                                     >  1:810403/1‑140 (MQ=255)

                   ggcggTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGt                                                                                                                    <  2:810403/140‑1 (MQ=255)

                     cggTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGtct                                                                                                                  <  1:1091798/140‑1 (MQ=255)

                         aTTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGACGGCAAAACGCATAATCCCATGTCTCGAc                                                                                                              >  1:724797/1‑140 (MQ=255)

                            ggCGACATTGATGATTTGGCGGCCCTGCGAGGCACTGTTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGtt                                                                                                           <  1:754574/140‑1 (MQ=255)

                            ggCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGtctcttatac                                                                                                           <  1:1260812/140‑7 (MQ=255)

                               gACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGt                                                                                                        <  1:1365281/140‑1 (MQ=255)

                                aCATTGATGATGTGGCGTCCCTGCGTGGCACTGGTGTGCTCGGCGTAAAAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTg                                                                                                       <  2:756790/140‑1 (MQ=255)

                                aCATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTg                                                                                                       <  2:1859744/140‑1 (MQ=255)

                                  aTTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGGCATCGCATTCCGACAAAACGCATAAACCCATGTCTCGACGTTCGTGAt                                                                                                     >  2:709317/1‑140 (MQ=255)

                                  aTTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGAt                                                                                                     >  2:1733145/1‑140 (MQ=255)

                                  aTTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGAt                                                                                                     >  2:1160857/1‑140 (MQ=255)

                                    tgatgaTGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATgg                                                                                                   >  2:3502/1‑140 (MQ=255)

                                     gatgatGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGt                                                                                                  <  2:1782059/140‑1 (MQ=255)

                                      atgatgTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTc                                                                                                 <  2:85612/140‑1 (MQ=255)

                                      atgatgTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTc                                                                                                 <  1:1160857/140‑1 (MQ=255)

                                        gatgTGGCGGCCCTGTGTGGCACTGTTGTTCGCGGCGCAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAg                                                                                               <  1:709317/140‑1 (MQ=255)

                                        gatgTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAg                                                                                               <  2:266948/140‑1 (MQ=255)

                                             ggcggcCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGt                                                                                                  <  2:303658/132‑1 (MQ=255)

                                             ggcggcCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGt                                                                                                  >  1:303658/1‑132 (MQ=255)

                                               cggcCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGa                                                                                        >  1:978277/1‑140 (MQ=255)

                                                ggcCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGt                                                                                                  >  2:343998/1‑129 (MQ=255)

                                                ggcCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGt                                                                                                  <  1:343998/129‑1 (MQ=255)

                                                ggcCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGaa                                                                                       <  1:1169767/140‑1 (MQ=255)

                                                     tGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCg                                                                                  >  2:1735832/1‑140 (MQ=255)

                                                      gCGTGGCACTGGTGTACGCGGCATACTAGGTGGTCGGCCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGt                                                                                 <  1:797931/140‑1 (MQ=255)

                                                       cGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTa                                                                                <  1:1890906/140‑1 (MQ=255)

                                                                ggTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGc                                                                       >  2:1182464/1‑140 (MQ=255)

                                                                 gtgtGCGCGGCGTAATAGTTGGTCGGGCATTTCTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCa                                                                      <  2:724797/140‑1 (MQ=255)

                                                                 gtgtGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTa                                                                                >  2:1124471/1‑130 (MQ=255)

                                                                 gtgtGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTa                                                                                <  1:1124471/130‑1 (MQ=255)

                                                                 gtgtGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCa                                                                      >  2:1871562/1‑140 (MQ=255)

                                                                 gtgtGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCa                                                                      >  2:1744043/1‑140 (MQ=255)

                                                                  tgtgCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCaa                                                                     <  2:238361/140‑1 (MQ=255)

                                                                   gtgCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAAc                                                                    >  2:1533517/1‑140 (MQ=255)

                                                                    tgCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAAcc                                                                   >  1:26804/1‑140 (MQ=255)

                                                                      cgcgGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCAt                                                                 >  1:284526/1‑140 (MQ=255)

                                                                      cgcgGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCAt                                                                 >  2:328788/1‑140 (MQ=255)

                                                                      cgcgGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCAt                                                                 >  1:1544383/1‑140 (MQ=255)

                                                                        cggcgTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGa                                                               <  2:1463292/140‑1 (MQ=255)

                                                                         ggcgTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCg                                                                        >  2:1074059/1‑130 (MQ=255)

                                                                         ggcgTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCg                                                                        <  1:1074059/130‑1 (MQ=255)

                                                                         ggcgTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGaa                                                              >  1:1541001/1‑140 (MQ=255)

                                                                          gcgTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGaaa                                                             <  1:1533517/140‑1 (MQ=255)

                                                                           cgTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAAt                                                            >  1:1518481/1‑140 (MQ=255)

                                                                           cgTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGATCGTGATGATCAGGTGACGAAAGGCTTACAGATTCGCAATCATGAAAt                                                            >  2:872370/1‑140 (MQ=255)

                                                                           cgTAATAGTTGGTCGGGCATTACTGGAAGATAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAAt                                                            >  2:966634/1‑140 (MQ=255)

                                                                                     ggTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTAAAGTATCGCAACCACGAAATCATTGGCGat                                                  >  1:745655/1‑140 (MQ=255)

                                                                                          ggCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCg                                                    >  2:1612514/1‑133 (MQ=255)

                                                                                          ggCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGt                                             >  2:1100623/1‑140 (MQ=255)

                                                                                                    gAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCa                                   >  2:480491/1‑140 (MQ=255)

                                                                                                      aGGTAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCaaa                                 >  2:1143332/1‑140 (MQ=255)

                                                                                                         tAAATTCACCGTGAAGGGGGCCCTCGCATGCTGGCGAAAAGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAacg                              <  2:745655/140‑1 (MQ=255)

                                                                                                         tAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAGGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAacg                              <  1:1791916/140‑1 (MQ=255)

                                                                                                         tAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAacg                              <  1:1735832/140‑1 (MQ=255)

                                                                                                         tAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAacg                              >  2:325457/1‑140 (MQ=255)

                                                                                                         tAAATTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCAGGAAGTCATTGGCTATATAGTGCCGCTGGCAAAacg                              >  2:499344/1‑140 (MQ=255)

                                                                                                         tAAATTCACCGTGAAGGAGGCCATCCCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAacg                              <  1:651640/140‑1 (MQ=255)

                                                                                                         aaaaTTCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGCGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAacg                              >  1:335927/2‑140 (MQ=255)

                                                                                                              tCACCGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAacgctacg                         <  2:1590025/140‑1 (MQ=255)

                                                                                                                  cGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTga                     >  2:1365903/1‑140 (MQ=255)

                                                                                                                  cGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAAGCATCGGCGATATCGTGCCGCTGGCAAAACGCTACGCTga                     >  2:572373/1‑140 (MQ=255)

                                                                                                                  cGTGAAGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCACAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTga                     >  1:1593045/1‑140 (MQ=255)

                                                                                                                      aaGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAGATCATTGGCGATATCGTGCCGCTGGCAAAacgctacg                         >  2:1284769/1‑132 (MQ=255)

                                                                                                                      aaGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAacgctacg                         <  1:1284769/132‑1 (MQ=255)

                                                                                                                      aaGGAGGCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTgaagaa                 >  1:153115/1‑140 (MQ=255)

                                                                                                                       aggaggCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGaagaag                >  2:78845/1‑140 (MQ=255)

                                                                                                                        ggaggCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAgg               <  2:1541001/140‑1 (MQ=255)

                                                                                                                         gaggCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTgaaga                  <  2:1106904/136‑1 (MQ=255)

                                                                                                                         gaggCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTgaaga                  >  1:1106904/1‑136 (MQ=255)

                                                                                                                         gaggCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTgaaga                  <  2:933802/136‑1 (MQ=255)

                                                                                                                         gaggCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTgaaga                  >  1:933802/1‑136 (MQ=255)

                                                                                                                            gCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGGTGGAAAGACGCTAGGCTGAAGAAGGCGCt           >  1:493980/1‑140 (MQ=255)

                                                                                                                            gCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCt           >  2:1555425/1‑140 (MQ=255)

                                                                                                                            gCCATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCt           >  1:1201120/1‑140 (MQ=255)

                                                                                                                             ccATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTg          <  1:1143332/140‑1 (MQ=255)

                                                                                                                              cATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGa         >  1:279592/1‑140 (MQ=255)

                                                                                                                              cATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGa         <  2:1263177/140‑1 (MQ=255)

                                                                                                                              cATCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGa         <  1:1382938/140‑1 (MQ=255)

                                                                                                                                tCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCTGCTAGCAAAACGTTACGATGAAGAAGGCGCTGACg       >  2:585767/1‑140 (MQ=255)

                                                                                                                                tCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTgaag                   <  1:1026195/128‑1 (MQ=255)

                                                                                                                                tCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTgaag                   >  2:1026195/1‑128 (MQ=255)

                                                                                                                                tCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACg       >  1:130076/1‑140 (MQ=255)

                                                                                                                                tCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACg       >  1:1774670/1‑140 (MQ=255)

                                                                                                                                tCGCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACg       >  1:1271279/1‑140 (MQ=255)

                                                                                                                                  gCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGaa     <  2:1774670/140‑1 (MQ=255)

                                                                                                                                  gCATGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGaa     <  1:1182464/140‑1 (MQ=255)

                                                                                                                                   cATGCCGGCAAAACGCATAATCCCACGTCCCGACGTTTGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAAc    <  1:585767/140‑1 (MQ=255)

                                                                                                                                     tGCCGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTg  <  2:279592/140‑1 (MQ=255)

                                                                                                                                     tGCCGGCAAAACGCACAATCCCCTGCCTCTACGTTCGAGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTg  <  1:499344/140‑1 (MQ=255)

GGTGGCATTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTG  >  NC_000913/2095712‑2095984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`