Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,753,415	Δ1 bp	coding (416/2103 nt)	ydhV ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,753,412	0	C	.	97.1%	129.0 / 0.2	35	coding (419/2103 nt)	ydhV	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base . (17/16);  minor base G (0/1);  total (17/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACC  >  NC_000913/1753273‑1753545                                                                                                                                            |                                                                                                                                      cGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTc                                                                                                                                       >  2:30319/1‑140 (MQ=255)      cgccgcTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTcccata                                                                                                                                  >  2:615088/1‑137 (MQ=255)       gccgcTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTcccataa                                                                                                                                 >  1:722739/1‑136 (MQ=255)                           tCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGc                                                                                                            <  2:28502/140‑1 (MQ=255)                           tCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGc                                                                                                            <  2:483834/140‑1 (MQ=255)                            cAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCt                                                                                                           <  2:722739/140‑1 (MQ=255)                                  gCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACttt                                                                                                     >  1:443572/1‑140 (MQ=255)                                    cAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCttttt                                                                                                                  >  2:632782/1‑125 (MQ=255)                                    cAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCttttt                                                                                                                  <  1:632782/125‑1 (MQ=255)                                     agagagagGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTc                                                                                                  <  1:615088/140‑1 (MQ=255)                                       agagagGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCAt                                                                                                <  2:168692/140‑1 (MQ=255)                                       agagagGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCAt                                                                                                >  2:530648/1‑140 (MQ=255)                                           agGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCttt                                                                                            <  1:30319/140‑1 (MQ=255)                                                       ttCCCCAGCCTTACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTGTTTCCAGTCTAGCTGTGTCATTTTTAATCTTCAGCCa                                                                                >  1:191345/1‑140 (MQ=255)                                                                   aCCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGAttt                                                                    >  2:600373/1‑140 (MQ=255)                                                                   aCCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGAttt                                                                    >  1:734739/1‑140 (MQ=255)                                                                   aCCAATAGCCGCCACACAGGTTTCAGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGAttt                                                                    >  2:681730/1‑140 (MQ=255)                                                                      aaTAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGc                                                                 <  1:530648/140‑1 (MQ=255)                                                                        tAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTGTGTGATCATTAATCTTTAGCCTTACCGGTGATTTCGcct                                                               >  2:198827/1‑140 (MQ=255)                                                                             gccACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGccttccct                                                          >  2:401315/1‑140 (MQ=255)                                                                             gccACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGccttccct                                                          >  2:174052/1‑140 (MQ=255)                                                                             gccACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGccttccct                                                          >  2:642631/1‑140 (MQ=255)                                                                                  acaGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGat                                                     <  2:443572/140‑1 (MQ=255)                                                                                         tCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGGTTACGCCTTGCCTTCGATAATAATg                                              >  1:206672/1‑140 (MQ=255)                                                                                           cGGACTGGTCAATCGACCAATTCCTTCCGTCTTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGAc                                            <  2:191345/140‑1 (MQ=255)                                                                                                  gTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTAt                                     >  1:395557/1‑140 (MQ=255)                                                                                                      aTCGACAAATTTCTTCCGTCGTTGCGCGCATCCC‑TTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAg                                 <  1:198827/140‑1 (MQ=255)                                                                                                      aTCGACAAATTTCTTCCGTCGTGGCCCGCATCCC‑TTTGCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAg                                 <  1:260103/140‑1 (MQ=255)                                                                                                                   ttcCGTCGTTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTg                    >  2:501619/1‑140 (MQ=255)                                                                                                                        tcgtTGCGCGCGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTg               >  2:569093/1‑140 (MQ=255)                                                                                                                             gcgcgcGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCaaaa          <  1:174052/140‑1 (MQ=255)                                                                                                                             gcgcgcGTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCaaaa          <  1:642631/140‑1 (MQ=255)                                                                                                                                 gcgTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGc      <  1:401315/140‑1 (MQ=255)                                                                                                                                 gcgTCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGc      <  2:734739/140‑1 (MQ=255)                                                                                                                                  cgTCCCTTTT‑CCCATGAGAAATGGGGTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGcc     <  1:322151/140‑1 (MQ=255)                                                                                                                                    tCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTg                    <  1:547339/123‑1 (MQ=255)                                                                                                                                    tCCCTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTg                    >  2:547339/1‑123 (MQ=255)                                                                                                                                     cccTTTT‑CCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGCATCCAGCGAATGTCATTTGCGATGCAAAAAAGCCAcc  >  1:342938/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                      CGCACCGCCGCTGTGGTTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACC  >  NC_000913/1753273‑1753545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑