Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,336,634	(T)7→6	coding (11/1689 nt)	yfaA ←	DUF2138 domain‑containing protein YfaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,336,628	0	T	.	100.0%	133.1 / NA	32	coding (17/1689 nt)	yfaA	DUF2138 domain‑containing protein YfaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (9/23);  total (9/23)

TGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAG  >  NC_000913/2336497‑2336758                                                                                                                                    |                                                                                                                                   tgttgtGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCttttttcac                                                                                                                            <  1:496754/140‑4 (MQ=255)       gccccaccaGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCa                                                                                                                     <  1:104118/137‑1 (MQ=255)           caccaGCGGCGCCAGCGTTTTTTCTGGCCCACTGCCAGAATATTGCAACGCCCAGACCCCAGCGGAAAGCAGTGCTATTGCGCCAAACCCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGc                                                                                                                 <  2:561080/140‑1 (MQ=255)             ccggcggcgACAGCGTTTTTTCCGGCCCACTGCCCGATTATTGCAACGCCGAGACCCCAGGGCAAGGCAGTGCTTTGCCTCCAAAGCCTACAACGCGAAAGATCCGGCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCca                                                                                                               <  2:656682/137‑1 (MQ=255)             ccaGCGGTGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCca                                                                                                               >  2:561667/1‑140 (MQ=255)               aGCGGCGACAACGTTTTTTCTGACCCACTGCGAGCTTATTGCAACGCCAAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCcaca                                                                                                             <  2:231961/140‑1 (MQ=255)                gcggcgACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACAt                                                                                                            <  1:436862/140‑1 (MQ=255)                gcggcgACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACAt                                                                                                            <  2:407739/140‑1 (MQ=255)                gcggcgACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCACCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACAt                                                                                                            <  1:137188/140‑1 (MQ=255)                  ggcgACAACGTTTTTTCTGGCCTACCGCCTGCATATTTCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑‑TTTTTCACCACTCATTGCCACATTcc                                                                                                         <  1:196161/140‑1 (MQ=255)                   gcgACAACGTTTTTTCTGGCCCTCTGCCAGCATATCGCACCGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTcc                                                                                                         <  2:278451/140‑1 (MQ=255)                   gcgACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTcc                                                                                                         <  1:53322/140‑1 (MQ=255)                    cgACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAACCCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCt                                                                                                        <  2:551080/140‑1 (MQ=255)                                   gggCCCACTGCCAGCAATTTGAGACGCCCATCCCCTAGCAGAAAGCAGTGTTATTGCGCCAAACCCTACAAGACCAAAAAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATagccagcc                                                                                         <  2:360587/139‑1 (MQ=255)                                           tGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTa                                                                                 <  1:561667/140‑1 (MQ=255)                                                 cATATTGCAACGCCCCGACGCCGGCTGAATGCAGTGCTATTGCGCCAAAACCTACAAGCCCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGca                                                                           <  1:240929/140‑1 (MQ=255)                                                    atTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAg                                                                        <  1:623373/140‑1 (MQ=255)                                                           cGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACt                                                                 >  2:110095/1‑140 (MQ=255)                                                                aGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAACCCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTAcc                                                            <  2:79592/140‑1 (MQ=255)                                                                                 aGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACaa                                           <  1:81640/140‑1 (MQ=255)                                                                                              aaaacCCTACAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACttt                              <  2:384867/139‑1 (MQ=255)                                                                                               aaaaCCCACAAGGCCAGGAAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCCGCCATTTTTTGCGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTg                             <  2:450774/140‑1 (MQ=255)                                                                                                      aCAAGACCATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTg                      >  1:656953/1‑140 (MQ=255)                                                                                                             cATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACtttt               >  2:722435/1‑140 (MQ=255)                                                                                                             cATAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACtttt               >  2:429252/1‑140 (MQ=255)                                                                                                              aTAGAACCGCTAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACCTTCCTTGTGCATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCATGCCCTAATACGACAAAAGCCCAGACTTTGCCGCCTGGACTTttc              >  2:161889/1‑140 (MQ=255)                                                                                                               tAGAACCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTttca             >  2:717183/1‑140 (MQ=255)                                                                                                                 aaaCCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTttcaat           <  2:151653/139‑1 (MQ=255)                                                                                                                 aaaCCGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTttcaat           <  1:292059/139‑1 (MQ=255)                                                                                                                    ccGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTttcaattca        >  1:13003/1‑140 (MQ=255)                                                                                                                    ccGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTttcaattca        >  2:689262/1‑140 (MQ=255)                                                                                                                     cGCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTttcaattcaa       >  1:24803/1‑140 (MQ=255)                                                                                                                       ccAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAAc     <  1:110095/140‑1 (MQ=255)                                                                                                                          gCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAg  <  2:13003/140‑1 (MQ=255)                                                                                                                          gCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAg  <  1:689262/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   TGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAG  >  NC_000913/2336497‑2336758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑