Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,947,343	(A)8→7	intergenic (‑165/‑44)	mltA ← / → metZ	membrane‑bound lytic murein transglycosylase A/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,947,336	0	A	.	100.0%	83.4 / NA	21	intergenic (‑158/‑51)	mltA/metZ	membrane‑bound lytic murein transglycosylase A/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (4/17);  total (4/17)

AAAAAATAAGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTA  >  NC_000913/2947197‑2947467                                                                                                                                            |                                                                                                                                    aaaaaaTAAGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTa                                                                                                                                     >  1:636212/1‑140 (MQ=255)                  gAAGATAACAAACCGCCGCGTTGAATGAAATGAGCAGCCGCTCCCTGATGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGaa                                                                                                                   <  2:298558/140‑1 (MQ=255)                       aaaCAAACCGCCGCGTTTAATGGAAGGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                              <  1:280179/139‑1 (MQ=255)                         aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                            <  2:408928/140‑1 (MQ=255)                         aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                            <  1:430016/140‑1 (MQ=255)                         aCAAACCGACGCGTTTCATGAAATGAGCAGGCTGTCCCTTACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                            <  2:349640/140‑1 (MQ=255)                           aaaCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGc                                                                                                          <  1:90579/140‑1 (MQ=255)                           aaaCCGCCGCGTTTAATGAAATGAGCAGC‑‑CTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                            <  1:560774/136‑1 (MQ=255)                           aaaCCGCCGCGTTTAATGAAATGAGCAGC‑‑CTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                            >  2:560774/1‑136 (MQ=255)                            aaCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCg                                                                                                         <  2:618666/140‑1 (MQ=255)                             aCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                            >  1:593466/1‑136 (MQ=255)                             aCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                            <  2:593466/136‑1 (MQ=255)                              ccgccgCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGa                                                                                                       <  1:377332/140‑1 (MQ=255)                                 ccgcGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGta                                                                                                    <  2:91446/140‑1 (MQ=255)                                                    cagcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGAcg                                                                                 <  2:630926/140‑1 (MQ=255)                                                    cagcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGAcg                                                                                 <  2:711797/140‑1 (MQ=255)                                                            cccTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTgg                                                                         >  2:46033/1‑140 (MQ=255)                                                              cTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGag                                                                       <  2:581246/140‑1 (MQ=255)                                                                 aCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAGAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGagcag                                                                    <  1:685450/140‑1 (MQ=255)                                                                    cAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCt                                                                 >  2:89420/1‑140 (MQ=255)                                                                                                     tgtttttgttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgccgt                                <  1:59463/140‑1 (MQ=255)                                                                                                                                   gATCGGGGT‑AAAAAAAGGTTGCCTGCAAACGCGAGCGTAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTa  <  1:196093/140‑1 (MQ=21)                                                                                                                                            |                                                                                                                                    AAAAAATAAGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTA  >  NC_000913/2947197‑2947467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑