| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,300,074 | Δ1 bp | coding (109/303 nt) | yhbQ → | DNA damage response nuclease YhbQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,300,071 | 0 | G | . | 97.8% | 185.3 / ‑2.6 | 46 | coding (106/303 nt) | yhbQ | DNA damage response nuclease YhbQ |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (21/24); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGG > NC_000913/3299936‑3300206 | tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGGTATAAGGAGCACCAAAGCGGCAAAgggcg > 1:206804/1‑138 (MQ=255)tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggcg > 2:12209/1‑138 (MQ=255)tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggcg > 1:602922/1‑138 (MQ=255)tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggcg > 2:481000/1‑138 (MQ=255)tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggcg > 2:597490/1‑138 (MQ=255)tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGCCGATCGCAGCTATCAGCAGCACCAAAGCGGCAAAgggcg > 2:304347/1‑138 (MQ=255) ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggcgaa > 1:110762/1‑136 (MQ=255) ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggcga > 2:693272/1‑136 (MQ=255) ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggcga < 1:693272/139‑4 (MQ=255) ccTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAg < 2:731499/140‑1 (MQ=255) cTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGAATGTCGAAAGCCGCGATCAGCAGCACCAAACCGGCAAA‑GGGCGAAAGc > 2:331875/1‑140 (MQ=255) tGACCTTAACATGCGACTCAGAAGATGACACCCTGTTTTCTTTACTTGCTCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCa < 2:225534/140‑1 (MQ=255) aCCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGGTATCAGCGGCAGCATAGCGGCAAA‑GGGCGAAAGctgt > 1:195735/1‑137 (MQ=255) aCCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACt > 2:523413/1‑140 (MQ=255) aCCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACt > 2:413349/1‑140 (MQ=255) aCCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACt > 1:170886/1‑140 (MQ=255) aCCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCAGCTTTCAACAGCACCAGAGCGGCAAA‑GGGCGAAAGCACt > 2:267641/1‑140 (MQ=255) ttAACATGCGACGCAGACGATGACACCCTGGTTTCTTTAATTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTgcg < 2:175143/140‑1 (MQ=255) aCATGCGACGCAGACGATGACATCCTGGTTTCTTTCCTTTATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCgg < 2:206804/140‑1 (MQ=255) aCATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCgg < 1:143806/140‑1 (MQ=255) aCATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCgg > 2:611539/1‑140 (MQ=255) cGACGCAGACGATGACACCCTGTTTTCTTTAGTTGGTCCCTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAg < 2:287923/140‑1 (MQ=255) cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGGTATCAGCAACACCAAATCGGCAAA‑GGGCGACAGCACTGCGCGGGAAAg > 2:233253/1‑140 (MQ=255) cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAg < 1:393206/140‑1 (MQ=255) gACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAgg < 1:481000/140‑1 (MQ=255) cGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGgag < 1:523413/140‑1 (MQ=255) cGATGACACCCTGGTCTCTTTACTTGATCCGTACCGCGGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTaa < 1:267641/140‑1 (MQ=255) aTGACACCTTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGGACTGCGCGGG‑AAGGAGAACTAACGc < 1:661823/140‑1 (MQ=255) aTGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACg < 2:173869/140‑1 (MQ=255) acCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGgt > 2:105440/1‑140 (MQ=255) tttACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCgcgc < 1:539459/140‑1 (MQ=255) tttACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCgcgc < 1:459446/140‑1 (MQ=255) gATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTggg < 1:413349/140‑1 (MQ=255) ccgACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGc > 1:442604/1‑140 (MQ=255) ccgACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGc > 1:454938/1‑140 (MQ=255) tAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGtt < 1:83194/140‑1 (MQ=255) gCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACg > 1:738190/1‑140 (MQ=255) cTTTATACCGGGATCGCCACGGGTGACGATCGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACgg < 1:307638/140‑1 (MQ=255) ttATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGc < 2:732179/140‑1 (MQ=255) ataCCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCgg > 2:439852/1‑140 (MQ=255) ataCCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCgg > 2:665302/1‑140 (MQ=255) cGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAata < 1:105440/140‑1 (MQ=255) cGGGATCACCACGGACGTCGAACGCTGCTATCAGCAGGACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAata < 1:304347/140‑1 (MQ=255) ggATCACCACGGATGGCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGCAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATc > 2:163246/1‑140 (MQ=255) gATCACCACGGATGTCGAACGCCGCTATCAGCGGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATcg < 2:697939/140‑1 (MQ=255) accacGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGtt > 1:374036/1‑140 (MQ=255) ccacGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTa < 1:554745/140‑1 (MQ=255) ggATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCa > 2:526284/1‑140 (MQ=255) gATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCTTTCGCTGGCGTAACAGGCGGAATATCGCGTTAAGCaa > 1:205933/1‑140 (MQ=255) tCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGa > 2:135878/1‑140 (MQ=255) aTCAGCAGCACCAAAGCGGC‑AAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCaaa < 1:314659/140‑1 (MQ=255) gcagcaCCAAAGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAaga > 2:439616/1‑140 (MQ=255) aaGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTACGTAACTGACCCAACGGGAAAAAGATCGTCTGGt > 2:347669/1‑140 (MQ=255) aaGCGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGt > 2:501500/1‑140 (MQ=255) cGGCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGc < 1:439852/140‑1 (MQ=255) gCAAA‑GGGCGAAAGCGCTGCGCCGGAAAGTAGAACTACCGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCgg < 1:298244/140‑1 (MQ=255) gCAAA‑GGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGAGCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCgg < 2:136818/140‑1 (MQ=255) | TGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGG > NC_000913/3299936‑3300206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |