Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,407,296	Δ1 bp	intergenic (+30/‑79)	accC → / → yhdT	biotin carboxylase/DUF997 domain‑containing protein YhdT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,407,293	0	T	.	100.0%	180.9 / NA	43	intergenic (+27/‑82)	accC/yhdT	biotin carboxylase/DUF997 domain‑containing protein YhdT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/24);  total (19/24)

TATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCT  >  NC_000913/3407155‑3407421                                                                                                                                           |                                                                                                                                  tATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAAATCGGTCTTCAGGGAAAATAAGACTGATAAAGCGTCAAAAGGCCGGAtt                                                                                                                                  >  2:256820/1‑140 (MQ=255)        aCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGcc                                                                                                                          <  2:511443/140‑1 (MQ=255)        aCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGcc                                                                                                                          <  1:689420/140‑1 (MQ=255)        aCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGcc                                                                                                                          <  1:29861/140‑1 (MQ=255)           aaCGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCttt                                                                                                                       >  1:545520/1‑140 (MQ=255)           aaCGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCttt                                                                                                                       >  1:718052/1‑140 (MQ=255)              gTTGATCTGCAGATCTGCATCATGAATGACGAGAACTACCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCtttttt                                                                                                                    <  1:213345/140‑1 (MQ=255)               ttGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTtta                                                                                                                   <  2:158091/140‑1 (MQ=255)               ttGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTtta                                                                                                                   <  1:458229/140‑1 (MQ=255)               ttGATCTGCAGATCCGCATCATAAATGACTAGAACTTACAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTtta                                                                                                                   <  1:219075/140‑1 (MQ=255)                tGATCTGCAGATCCGCATCATGCATCACGTGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTttat                                                                                                                  <  1:303182/140‑1 (MQ=255)                       cAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTggg                                                                                                           >  1:729429/1‑140 (MQ=255)                              ggataatgaatggCGAGAACGTCCAACATTGCGGCACTAACAACCACTATTCTGAGGAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGAc                                                                                                    <  2:24540/135‑1 (MQ=255)                              gCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGAc                                                                                                    <  2:403470/140‑1 (MQ=255)                              gCATCATGAATGACGAGAACTTCCAGCATGGTGGCACCAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGAc                                                                                                    <  1:256820/140‑1 (MQ=255)                                atcatGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACaa                                                                                                  <  2:35510/140‑1 (MQ=255)                                 tcatGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑c                                                                                                >  1:171990/1‑140 (MQ=255)                                      aatgaCGAGAACTTCCAGCATGGCGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑ccc                                                                                              >  1:63335/1‑137 (MQ=255)                                      aatgaCGAGAACTTCCAGCATGGCGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑ccc                                                                                              <  2:63335/137‑1 (MQ=255)                                      aatgaCGAGAACTTCCAGCATGGCGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCa                                                                                           >  1:170628/1‑140 (MQ=255)                                        tgaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATa                                                                                         <  2:449964/140‑1 (MQ=255)                                                            gtggCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTttct                                                                     >  2:31380/1‑140 (MQ=255)                                                                           aCTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGAc                                                      <  2:365441/140‑1 (MQ=255)                                                                               tCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaaa                                                  >  2:14603/1‑140 (MQ=255)                                                                               tCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaaa                                                  >  2:273639/1‑140 (MQ=255)                                                                                   gagaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTCCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATg                                              >  2:692235/1‑140 (MQ=255)                                                                                    agaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATgg                                             >  2:731154/1‑140 (MQ=255)                                                                                      aaaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGa                                            <  2:729429/140‑1 (MQ=255)                                                                                        aaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGacac                                         <  2:545520/140‑1 (MQ=255)                                                                                             tCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtt                                    <  2:718052/140‑1 (MQ=255)                                                                                                  cTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGtt                               <  1:31380/140‑1 (MQ=255)                                                                                                     cAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAg                            <  1:392460/140‑1 (MQ=255)                                                                                                     cAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAg                            >  2:460667/1‑140 (MQ=255)                                                                                                                    tGCTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAG‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGgcg             <  2:134847/140‑1 (MQ=255)                                                                                                                      cTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAG‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGgcgcg           >  2:517386/1‑140 (MQ=255)                                                                                                                      cTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAg                            >  2:407331/1‑123 (MQ=255)                                                                                                                      cTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAg                            <  1:407331/123‑1 (MQ=255)                                                                                                                      cTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGgcgcg           >  1:439708/1‑140 (MQ=255)                                                                                                                      cTAAAGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGGACAATCCCCGCTTTCTTCACCCATCTGGGACAAATAATTGACGCTCGTTTTATTCAAGCCCATAAAGAGgcgcg           >  2:254759/1‑140 (MQ=255)                                                                                                                        aaaGCGTCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgct         <  1:692235/140‑1 (MQ=255)                                                                                                                              tCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcgc   >  1:570366/1‑140 (MQ=255)                                                                                                                              tCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcgc   >  2:26662/1‑140 (MQ=255)                                                                                                                              tCAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACCCGTTTTGGTCAGGCTCATAAAGAGGCgcgctgggcgc   >  1:272218/1‑140 (MQ=255)                                                                                                                               cAAAAGGCCGGA‑TTTCCGGCCTTTTTTATTACTGGCGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCATCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcgct  <  2:246433/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                  TATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAA‑CCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCT  >  NC_000913/3407155‑3407421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑