Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	548,964	Δ1 bp	coding (608/1260 nt)	ylbE →	DUF1116 domain‑containing protein YlbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	548,961	0	C	.	100.0%	141.7 / NA	34	coding (605/1260 nt)	ylbE	DUF1116 domain‑containing protein YlbE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (20/14);  total (20/14)

AGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGGCCCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGA  >  NC_000913/548827‑549095                                                                                                                                       |                                                                                                                                       aGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGGcccaca                                                                                                                                   >  2:522778/1‑137 (MQ=255) aGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGGcccaca                                                                                                                                   >  2:46146/1‑137 (MQ=255) aGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGGcccaca                                                                                                                                   >  1:700620/1‑137 (MQ=255)   tATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACaaa                                                                                                                                >  1:657089/1‑140 (MQ=255)         gcgcGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTc                                                                                                                          <  2:657089/140‑1 (MQ=255)          cgcgGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTCCGCTGG‑CCCAAAAATTACTCg                                                                                                                         >  1:732877/1‑140 (MQ=255)            cggcgCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGcc                                                                                                                       <  1:5919/140‑1 (MQ=255)             ggcgCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCt                                                                                                                      >  1:72718/1‑140 (MQ=255)                gcTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGa                                                                                                                   <  1:556657/140‑1 (MQ=255)                gcTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGa                                                                                                                   <  1:671464/140‑1 (MQ=255)                   ggggCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCa                                                                                                                <  2:543621/140‑1 (MQ=255)                     ggCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATg                                                                                                              <  1:694557/140‑1 (MQ=255)                         cATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATaa                                                                                                          >  2:84781/1‑140 (MQ=255)                         cATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATaa                                                                                                          >  2:33090/1‑140 (MQ=255)                         cATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATaa                                                                                                          >  2:25379/1‑140 (MQ=255)                              aGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGc                                                                                                     >  1:40401/1‑140 (MQ=255)                                             tcACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGa                                                                                      >  2:583831/1‑140 (MQ=255)                                               aCGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATg                                                                                    >  2:550891/1‑140 (MQ=255)                                                 ggCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGa                                                                                  <  1:522778/140‑1 (MQ=255)                                                    gatgatGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGAttt                                                                               >  1:545226/1‑140 (MQ=255)                                                       gatgGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCt                                                                            <  2:581830/140‑1 (MQ=255)                                                               aGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGa                                                                    <  1:46146/140‑1 (MQ=255)                                                                    aTTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGAt                                                               <  2:72718/140‑1 (MQ=255)                                                                           tGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGttct                                                        >  2:604970/1‑140 (MQ=255)                                                                                        ccATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTcgc                                           >  1:362374/1‑140 (MQ=255)                                                                                          aTCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGa                                         <  1:33090/140‑1 (MQ=255)                                                                                          aTCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGa                                         <  1:583831/140‑1 (MQ=255)                                                                                               cGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCt                                    <  1:726646/140‑1 (MQ=255)                                                                                                 cAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTa                                  <  2:700620/140‑1 (MQ=255)                                                                                                       tGCTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCaa                            >  2:83174/1‑140 (MQ=255)                                                                                                         cTTCCTCTGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAgg                          >  2:417767/1‑140 (MQ=255)                                                                                                              tctGCACTGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCGACCTCGCGATGGCGTACTGCAAGgcggcg                     >  1:353512/1‑140 (MQ=255)                                                                                                                     tGTTAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCgatggatg              >  1:417042/1‑140 (MQ=255)                                                                                                                       ttAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCt            >  2:538404/1‑140 (MQ=255)                                                                                                                       ttAATGCGTGCGCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCt            >  1:403693/1‑140 (MQ=255)                                                                                                                            gcgtgcgCTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGgcgc       >  2:162727/1‑140 (MQ=255)                                                                                                                                 cgcTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCgatga  >  1:389361/1‑140 (MQ=255)                                                                                                                                 cgcTGG‑CCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGATTACTGCAAGGCGGCGATGGATGCTGGCGCgatga  >  2:280785/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       AGTATTAAGCGCGGCGCTGGGGCGCATGGAGCGCGGTATCGATCTCACGGCGATGATGGCGCAGGGCATTACGATGGGCGATGAGTTCCATCAACGCAATATTGCTTCCTCTGCACTGTTAATGCGTGCGCTGGCCCCACAAATTGCTCGCCTCGATCATGATAAACAGCACATCGCCGAAGTGATGGATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGA  >  NC_000913/548827‑549095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑