Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,419,193	Δ1 bp	coding (433/1065 nt)	ulaG ←	L‑ascorbate‑6‑phosphate lactonase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,419,190	0	T	.	100.0%	123.7 / NA	30	coding (436/1065 nt)	ulaG	L‑ascorbate‑6‑phosphate lactonase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/16);  total (14/16)

GGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGGTTTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCTGCATCACGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGAT  >  NC_000913/4419051‑4419318                                                                                                                                            |                                                                                                                                 ggcaggGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGGt                                                                                                                                  <  2:371331/140‑1 (MQ=255)       gTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGGtttcggt                                                                                                                            <  2:4355/140‑5 (MQ=255)         gATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCACTTTCGGTACGCCCTAAACAATCCAAAAATGTACACAGG‑TTTCGGTcc                                                                                                                         >  2:336277/1‑140 (MQ=255)          aTCAGTGCAGTACGGTCGAAAGTATGAAGTGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCg                                                                                                                        <  1:336277/140‑1 (MQ=255)           tCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGa                                                                                                                       >  1:680969/1‑140 (MQ=255)                    tACGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCGATCGACACATCCACACTGG‑TTTCGGTCCGATAAACGGTa                                                                                                              >  2:228712/1‑140 (MQ=255)                    tACGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTa                                                                                                              >  1:497995/1‑140 (MQ=255)                    tACGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACACCCCAGCCTATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTa                                                                                                              >  2:283610/1‑140 (MQ=255)                     aCGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTAc                                                                                                             <  2:643677/140‑1 (MQ=255)                     aCGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTAc                                                                                                             <  2:370100/140‑1 (MQ=255)                     aCGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTAc                                                                                                             <  2:497995/140‑1 (MQ=255)                      cGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCAACAAATCTACACAGG‑TTTCGGGCCGATAAACGGTACg                                                                                                            >  1:294108/1‑140 (MQ=255)                        gTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTACGTc                                                                                                          <  2:433549/140‑1 (MQ=255)                         tCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGGtttcggt                                                                                                                            <  2:494494/122‑5 (MQ=255)                         tCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGGtttcggt                                                                                                                            >  1:494494/1‑118 (MQ=255)                          cGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTACGTCAt                                                                                                        <  2:437003/140‑1 (MQ=255)                          cGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTACGTCAt                                                                                                        <  2:6951/140‑1 (MQ=255)                               gCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTACGTCATCTGca                                                                                                   <  2:180354/140‑1 (MQ=255)                                   cAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACTGG‑TTTCGATCCGATCAACGGTACGTCATCTGCACAAt                                                                                               >  1:251562/1‑140 (MQ=255)                                                 tCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCTGCATCACGgca                                                                                 <  2:134663/140‑1 (MQ=255)                                                                          cGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCTGCATCACGGCAGCAGCGACGTTAACGTCGATATGAt                                                        <  2:680969/140‑1 (MQ=255)                                                                                        aCGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCTGCATCACGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGa                                          <  2:41158/140‑1 (MQ=255)                                                                                                    tctTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTACGTGATCTGCACAATTGTGCATCACGGCTGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGCGTGGCCAGTAcc                              >  2:457994/1‑140 (MQ=255)                                                                                                      tttCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCTGCATCACGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGAGTCGCCAGTACcgc                            >  2:658480/1‑140 (MQ=255)                                                                                                       ttCGGTACGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCTGCATCACGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGAGTCGCCAGTACcgcg                           <  1:410173/140‑1 (MQ=255)                                                                                                             aCGCCCCAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCTGCATCACGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATc                     >  1:570822/1‑140 (MQ=255)                                                                                                                ccccAGCCAATCCACAAATCCACACAGG‑TTTCGGTTCGACAAACGGTAAGTCATCTGCACAATTCTGCATCACGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTgg                  <  2:251562/140‑1 (MQ=255)                                                                                                                ccccAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCTGCATCATGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTgg                  >  1:85950/1‑140 (MQ=255)                                                                                                                ccccAGCCAATCCACAAATCCACACAGG‑TTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCTGCATCACGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTgg                  >  1:530755/1‑140 (MQ=255)                                                                                                                         atccacaaatccacaCAGG‑TTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCTGCATCACGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCa         <  2:530755/140‑1 (MQ=255)                                                                                                                           ccacaaatccactCAGG‑TTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCTGCATCACGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCaaa       <  1:501057/140‑1 (MQ=255)                                                                                                                           ccacaaatccacaCAGG‑TTTCGGTCCGATAAACGGTATGTCATCTGCACAATTCTGCATCACGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCaaa       <  1:283610/140‑1 (MQ=255)                                                                                                                           ccacaaatccacaCAGG‑TTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCCGCATCACGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCaaa       >  2:368997/1‑140 (MQ=255)                                                                                                                                aatccacaCAGG‑TTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCTGCATCACGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGAt  >  1:5921/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                 GGCAGGGTGATCAGTGCAGTACGGTCGAAAGCATCAAGCGCATGAATTTCAATGTCTTTCACTTTTACTACATCGCCCGGTTTGACCACGATGCAACGCTCTTTCGGTACGCCCCAGCCAATCCACAAATCCACACAGGTTTTCGGTCCGATAAACGGTACGTCATCTGCACAATTCTGCATCACGGCAGCAGCGACGTTAACGTCGATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGAT  >  NC_000913/4419051‑4419318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑