| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,449,073 | (A)7→6 | intergenic (+31/+49) | chpB → / ← ppa | endoribonuclease toxin ChpB/inorganic pyrophosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,449,067 | 0 | A | . | 94.3% | 125.7 / 2.9 | 35 | intergenic (+25/+55) | chpB/ppa | endoribonuclease toxin ChpB/inorganic pyrophosphatase |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base . (20/13); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01 | |||||||||||
GTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCA > NC_000913/4448929‑4449201 | gTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaa > 1:413363/1‑140 (MQ=255)gTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaa > 1:685424/1‑140 (MQ=255) gAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTCTTTATTGATCGCGGGCATaaaa > 1:264833/1‑140 (MQ=255) gAATCAGGTGCGGATCATGGATTTATACGGCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaa < 1:309571/140‑1 (MQ=255) aaTCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGA‑‑AAGGTGTGTTTATTTATCGCGGGCATaaaaaac > 1:712004/1‑139 (MQ=255) tCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGGGGAATAAGGGGTATTATTTTATTGCGGGCATaaaaaac > 1:224718/1‑139 (MQ=255) aGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAccc < 2:685424/140‑1 (MQ=255) tGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGCGGTGGGATAAGGTGGGTTTATTTATCGCGGGCAT‑AAAAAACCCTTa > 1:538589/1‑140 (MQ=255) aTCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGcc > 2:461816/1‑140 (MQ=255) aTCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGcc < 1:80707/140‑1 (MQ=255) tCTACACGCCCGGCTCGCAAAGTGTCTTGGTCTGGCTTCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGccc < 1:318326/140‑1 (MQ=255) tACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTCTCGCGGGGAT‑ATAAAACCCTTACTAACCGAAGCCCgg > 1:290870/1‑140 (MQ=255) tACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGCGTGTTTATATATCGCGAGCAT‑AAAAGACCCGTACTAACCGAAGCCCgg > 2:233761/1‑140 (MQ=255) cGCCCGGCAGGCAAAGCGTATTGGTCTGGCTGCGGTTGATGTGGTGGAGGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGtt < 2:342986/140‑1 (MQ=255) gCCCGGCTGGCAAAGCGTATTGCTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTc < 1:233761/140‑1 (MQ=255) gCCCGGCTGGCAAAGCGTATTGATCTGGCTGCGGAAGAGATGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGtt < 2:264833/140‑1 (MQ=255) cccGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCa > 1:56037/1‑140 (MQ=255) ggCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGttat < 1:461816/140‑1 (MQ=255) tGGTCTGGGTGCGGATGAGGTGTTGGAAGAGTCTTTATTTCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑‑AAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAgaagaa < 2:352341/140‑1 (MQ=255) ggTCTGGCTGCGGATGATGTGGCGGAAGAGGCGTTTTTAAGCTTGCAGCCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAgaagaa < 2:224718/140‑1 (MQ=255) ggTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAgaagaa < 2:413363/140‑1 (MQ=255) tGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACtta > 1:62876/1‑140 (MQ=255) tGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGGGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACtta > 1:546724/1‑140 (MQ=255) gtggtgGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTgcgc < 2:62876/140‑1 (MQ=255) ggCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAaggag > 1:102750/1‑140 (MQ=255) tGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGc > 1:710344/1‑140 (MQ=255) gCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCt > 1:79719/1‑140 (MQ=255) aGGCGGTGGTGGAATAAGGGGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCttt > 2:724085/1‑140 (MQ=255) aGGTGTGTTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGttt > 1:445262/1‑140 (MQ=255) gtgtgtTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTc > 2:426023/1‑140 (MQ=255) gtgtgtTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTc > 1:522632/1‑140 (MQ=255) tgtTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCa > 1:107144/1‑140 (MQ=255) tgtTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCa > 2:173860/1‑140 (MQ=255) gtTTATTTATCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCaa > 2:157166/1‑140 (MQ=255) tttatCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTc < 1:82750/140‑1 (MQ=255) tttatCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTc < 1:496726/140‑1 (MQ=255) tCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTATCGGTTTCTGCGTTTTCCCAACCTTCAACt > 1:317000/1‑140 (MQ=255) tCGCGGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACt > 2:559281/1‑140 (MQ=255) gcgGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTccc < 1:735792/127‑1 (MQ=255) gcgGGCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTccc > 2:735792/1‑127 (MQ=255) ggCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCa < 2:412088/139‑1 (MQ=255) ggCAT‑AAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCa > 1:412088/1‑139 (MQ=255) | GTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCA > NC_000913/4448929‑4449201 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |