Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,567,286	(A)5→4	intergenic (‑40/+1)	yjiN ← / ← mdtM	DUF445 domain‑containing protein YjiN/multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,567,282	0	A	.	100.0%	93.2 / NA	23	intergenic (‑36/+5)	yjiN/mdtM	DUF445 domain‑containing protein YjiN/multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (18/5);  total (18/5)

TGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACC  >  NC_000913/4567147‑4567406                                                                                                                                        |                                                                                                                             tGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCTTGACGGCCGGATCGCGCaaaat                                                                                                                          >  1:229343/1‑139 (MQ=255)     cggcAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACt                                                                                                                     <  2:625900/140‑1 (MQ=255)     cggcAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACt                                                                                                                     <  2:429976/140‑1 (MQ=255)                                 gcagcGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATg                                                                                         >  1:133422/1‑140 (MQ=255)                                       gcgATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCGACTAGCTCGGCTGCCTGATGCTGgcg                                                                                   >  1:394698/1‑140 (MQ=255)                                                      gagagCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCGACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCaa                                                                    <  2:394698/140‑1 (MQ=255)                                                       agagCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAAt                                                                   >  2:642825/1‑140 (MQ=255)                                                          gcgcCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATccc                                                                >  1:693194/1‑140 (MQ=255)                                                           cgcCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCg                                                               >  1:72120/1‑140 (MQ=255)                                                            gcCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGTCTGCCTGATGCCGGCGCACGCGATTGAGCAATCCCGc                                                              >  1:295851/1‑140 (MQ=255)                                                                aCCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAgg                                                          >  1:138141/1‑140 (MQ=255)                                                                  cTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGt                                                        <  1:642825/140‑1 (MQ=255)                                                                  cTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGt                                                        <  2:68385/140‑1 (MQ=255)                                                                                        tGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAAcgcccgcc                                  >  1:63279/1‑140 (MQ=255)                                                                                                ttCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGc                          >  1:493267/1‑140 (MQ=255)                                                                                                    tAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAAc                      >  1:492455/1‑140 (MQ=255)                                                                                                      aCGTTAAGAATAGAGCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAg                    >  2:659096/1‑140 (MQ=255)                                                                                                                  gagCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCaa        >  2:373945/1‑140 (MQ=255)                                                                                                                   agCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAg       >  1:101324/1‑140 (MQ=255)                                                                                                                   agCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAg       >  1:179058/1‑140 (MQ=255)                                                                                                                   agCCTGACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAg       >  1:111457/1‑140 (MQ=255)                                                                                                                   agCCTGACGGCCGGATCGCGC‑AAAATCAATGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATATCGCGCGCCACAAAGGATTACAGATGAAACGGCAAg       >  2:275490/1‑140 (MQ=255)                                                                                                                        gACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGAcc  >  1:96697/1‑140 (MQ=255)                                                                                                                        gACGGCCGGATCGCGC‑AAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGAcc  >  1:52381/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                             TGGGCGGCAAAAACAGCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAAGAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACC  >  NC_000913/4567147‑4567406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑