Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,324,113	(T)7→6	coding (16/621 nt)	yciO →	putative RNA‑binding protein YciO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,324,107	0	T	.	100.0%	106.6 / NA	26	coding (10/621 nt)	yciO	putative RNA‑binding protein YciO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (11/15);  total (11/15)

CACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAA  >  NC_000913/1323971‑1324237                                                                                                                                         |                                                                                                                                   cacaAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGtttt                                                                                                                                 >  2:628322/1‑140 (MQ=255)  acaAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGttttt                                                                                                                                <  2:412865/140‑1 (MQ=255)          cTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCa                                                                                                                       >  1:35703/1‑140 (MQ=255)           tGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCAt                                                                                                                      >  1:531973/1‑140 (MQ=255)                  catcaGCCATGCCCGTGAATCGAACTGGGTCCTAAGCTCTGGCTGCCCGCAGGCGTCGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATa                                                                                                               <  2:323896/140‑1 (MQ=255)                       gCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAAcc                                                                                                            >  2:541896/1‑138 (MQ=255)                       gCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAAcc                                                                                                            <  1:541896/138‑1 (MQ=255)                                 tGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTc                                                                                                >  2:53904/1‑140 (MQ=255)                                     tCGAACTGGGTCGTAAATTCTGGCTGCCCGCTGGCCTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGAt                                                                                            <  2:291933/140‑1 (MQ=255)                                        aaCTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCaa                                                                                         >  2:621208/1‑140 (MQ=255)                                            gggTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAg                                                                                     <  1:176069/140‑1 (MQ=255)                                            gggTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAg                                                                                     <  1:184607/140‑1 (MQ=255)                                             ggTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAgg                                                                                    <  2:641296/140‑1 (MQ=255)                                             ggTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAgg                                                                                    <  2:531973/140‑1 (MQ=255)                                                        ctGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATc                                                                         <  2:387390/140‑1 (MQ=255)                                                               cccGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTa                                                                  <  2:599295/140‑1 (MQ=255)                                                                          tGAAGGCGTCTGGCAGCTATGGGAAAAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTg                                                       <  1:269874/140‑1 (MQ=255)                                                                                  tCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTg                                                       <  2:554950/132‑1 (MQ=255)                                                                                  tCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTg                                                       >  1:554950/1‑132 (MQ=255)                                                                                  tCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTa                                               >  2:23372/1‑140 (MQ=255)                                                                                       cAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCaa                                          <  1:621208/140‑1 (MQ=255)                                                                                              gggAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTc                                   <  1:28387/140‑1 (MQ=255)                                                                                                             acacCACAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCgg                    <  1:628322/140‑1 (MQ=255)                                                                                                              caccacAGAGAGGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGGTTAATCAACTGATTCCGGCTATGCGCCCGGc                   >  2:434380/1‑140 (MQ=255)                                                                                                                      agagGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGTTAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGAGGTGATTGGTTATCCACCTGATTCCGGCTACCCGCTCGGCTGTAAAAt           >  2:335836/1‑140 (MQ=255)                                                                                                                        agGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTg         >  1:125928/1‑140 (MQ=255)                                                                                                                           gAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAg      >  1:643436/1‑140 (MQ=255)                                                                                                                               tATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATaa  <  2:689242/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   CACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAA  >  NC_000913/1323971‑1324237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑