Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,352,747	Δ1 bp	coding (882/993 nt)	sapD ←	putrescine ABC exporter ATP binding protein SapD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,352,744	0	C	.	100.0%	93.6 / NA	23	coding (885/993 nt)	sapD	putrescine ABC exporter ATP binding protein SapD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (9/14);  total (9/14)

CAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCAGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGA  >  NC_000913/1352616‑1352876                                                                                                                                 |                                                                                                                                     cAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGcccagac                                                                                                                                <  2:533137/135‑5 (MQ=255) cAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGcccagac                                                                                                                                >  1:533137/1‑131 (MQ=255)  aaGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAAc                                                                                                                         >  2:103777/1‑140 (MQ=255)    gcagcGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCa                                                                                                                       <  2:31355/140‑1 (MQ=255)    gcagcGTTTCGATCATCTCACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCa                                                                                                                       <  2:118606/140‑1 (MQ=255)           ttCGATCATCTCACTCTTTCTCCATGTTCGGTGGGGAATTACAGGCATGGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTa                                                                                                                <  2:226509/140‑1 (MQ=255)                   tctcACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTcc                                                                                                        <  1:57203/140‑1 (MQ=255)                     tcACTCTTTCTCCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCcag                                                                                                      <  1:103777/140‑1 (MQ=255)                             tctcCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCTAGACGACCACCTATAGGTAACAGTTCCAGCAGTGGGa                                                                                              >  1:337291/1‑140 (MQ=255)                             tctcCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCcagcag                                                                                                   <  2:639953/135‑1 (MQ=255)                             tctcCATGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCcagcag                                                                                                   >  1:639953/1‑135 (MQ=255)                                   tGTTCGGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAgcgc                                                                                        >  2:138488/1‑140 (MQ=255)                                     ttcggCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCg                                                                                      >  1:639781/5‑140 (MQ=255)                                      tcggCGGGAAATGACAGTCATATAGATGATTCTTCCCCCGCGTCAAACTTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCgg                                                                                     <  2:289609/137‑1 (MQ=255)                                       cggCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCggg                                                                                    >  1:701716/3‑140 (MQ=255)                                       cggCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCggg                                                                                    >  1:469624/3‑140 (MQ=255)                                              aaaTGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTa                                                                             <  2:701716/140‑1 (MQ=255)                                                     aGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGc                                                                      <  2:476815/140‑1 (MQ=255)                                                        cATAGAGATGATTTTTCACCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGAc                                                                   <  2:296775/140‑1 (MQ=255)                                                                 gatTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCg                                                          >  2:660888/1‑140 (MQ=255)                                                                         gCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATcgcg                                                  <  2:469624/140‑1 (MQ=255)                                                                               gTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCg                                            <  2:639781/140‑1 (MQ=255)                                                                                                         tcGTTGTGCATACGGGCAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAgcgc                  <  1:559543/140‑1 (MQ=255)                                                                                                                         cAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCt                >  2:476648/1‑126 (MQ=255)                                                                                                                         cAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCt                <  1:476648/126‑1 (MQ=255)                                                                                                                         cAACGTGG‑CCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGgatga  >  1:494191/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                     CAAGCAGCGTTTCGATCATCTCACTCTTTCTCCATGTTCAGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGA  >  NC_000913/1352616‑1352876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑