Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,373,237	(T)6→5	coding (16/882 nt)	ycjO →	putative ABC transporter membrane subunit YcjO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,373,232	0	T	.	100.0%	89.0 / NA	22	coding (11/882 nt)	ycjO	putative ABC transporter membrane subunit YcjO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (12/10);  total (12/10)

TGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCT  >  NC_000913/1373101‑1373358                                                                                                                                    |                                                                                                                               tgAGTTCAATGGTGCATTACGTTACCGTGGGCAAAGCCGCTCTCTCTACTACGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCtttttcagg                                                                                                                        <  2:282832/140‑5 (MQ=255) tgAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCtttttcagg                                                                                                                        <  1:109633/140‑5 (MQ=255)                  aaCGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTG‑‑CGAACAGCACTAAACCCAGGACAGGAATCCGAAATGAACTGGC‑TTTTTAAGGTCGTTCCGATATGCCCttt                                                                                                    >  2:316689/1‑140 (MQ=255)                                 aaaGCCGATCTCTCTACTACGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTtc                                                                                       <  1:78973/140‑1 (MQ=255)                                        aTCTCTCTACTACGCTGTAAGCTAGCCAGAAAAAATTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGcc                                                                                <  2:280535/140‑1 (MQ=255)                                                    cGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGGTCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTAct                                                                    >  2:12901/1‑140 (MQ=255)                                                    cGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTAct                                                                    >  2:485466/1‑140 (MQ=255)                                                    cGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCATTCCGATACGCCATTTACGCTGCTGCTTCTCGCGCCCAGCTTATTAct                                                                    >  2:277582/1‑140 (MQ=255)                                                       tGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTActgct                                                                 >  2:377313/1‑140 (MQ=255)                                                       tGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTAc                                                                     <  2:684832/136‑1 (MQ=255)                                                       tGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTAc                                                                     >  1:684832/1‑136 (MQ=255)                                                        gCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTActgctg                                                                >  2:694491/1‑140 (MQ=255)                                                                 ccAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCtg                                                       >  1:408475/1‑140 (MQ=255)                                                                    gAAAAAACTGGATGATCTG‑‑CGATCAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGgtggc                                                  <  1:316689/140‑1 (MQ=255)                                                                           cTGGATGAACTGGTCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGgtggcgtggc                                             <  1:12901/140‑1 (MQ=255)                                                                              gATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCTGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGa                                          <  1:528097/140‑1 (MQ=255)                                                                              gATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGa                                          <  1:621043/140‑1 (MQ=255)                                                                              gATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGa                                          >  2:621043/1‑140 (MQ=255)                                                                                        tCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGaa                                >  1:44673/1‑140 (MQ=255)                                                                                                            gacaggaATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAg                    >  1:61041/1‑132 (MQ=255)                                                                                                            gacaggaATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAg                    <  2:61041/132‑1 (MQ=255)                                                                                                            gacaggaATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTAc            >  2:60173/1‑140 (MQ=255)                                                                                                                 gaATCCGCAATGAACAGGC‑TTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTg       <  1:377313/140‑1 (MQ=255)                                                                                                                      cGCAATGAACAGGC‑TTTTTCAGTTCGTTCTGATATGCTCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCt  <  1:285184/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                               TGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAGCCAGAAAAAACTGGATGAACTGATCGAACAGCACTAAACCCAGGACAGGAATCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCT  >  NC_000913/1373101‑1373358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑