Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,721	(G)7→6	intergenic (‑265/+44)	ydaG ← / ← racR	Rac prophage; uncharacterized protein YdaG/Rac prophage; DNA‑binding transcriptional repressor RacR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,715	0	G	.	100.0%	172.6 / NA	41	intergenic (‑259/+50)	ydaG/racR	Rac prophage; uncharacterized protein YdaG/Rac prophage; DNA‑binding transcriptional repressor RacR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (25/16);  total (25/16)

GCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCG  >  NC_000913/1419587‑1419847                                                                                                                                 |                                                                                                                                     gCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAAt                                                                                                                          >  1:643791/1‑140 (MQ=255)   ccGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATaa                                                                                                                        <  1:707788/140‑1 (MQ=255)     ggCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACg                                                                                                                      <  2:452954/140‑1 (MQ=255)     ggCGTTCTGTGGGGCGGGGTGGGTGCTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACg                                                                                                                      <  1:295778/140‑1 (MQ=255)       cGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGa                                                                                                                    >  1:602890/1‑140 (MQ=255)            tgtgGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑‑GGGGGTTAAATAACGGAATCCAg                                                                                                              >  1:139189/1‑140 (MQ=255)                        tgggtggTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGt                                                                                                   <  2:141327/140‑1 (MQ=255)                        tgggtggTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGt                                                                                                   <  2:691024/140‑1 (MQ=255)                                aTGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCat                                                                                           >  2:18701/1‑140 (MQ=255)                                aTGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCat                                                                                           >  1:318125/1‑140 (MQ=255)                                aTGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGATATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCat                                                                                           >  2:444146/1‑140 (MQ=255)                                    tGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaa                                                                                       >  2:61197/1‑140 (MQ=255)                                        aCTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaagttt                                                                                   <  1:403324/140‑1 (MQ=255)                                         cTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaagttta                                                                                  >  2:601967/1‑140 (MQ=255)                                           ataGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaagtttaag                                                                                >  2:585992/1‑140 (MQ=255)                                            taGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaagtttaagt                                                                               >  2:280872/1‑140 (MQ=255)                                                    gCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGttt                                                                       >  1:62060/1‑140 (MQ=255)                                                      cTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGttttt                                                                     >  1:738129/1‑140 (MQ=255)                                                         attgattgTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTtgtg                                                                  <  1:601967/140‑1 (MQ=255)                                                              ttgTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGtt                                                             <  1:585992/140‑1 (MQ=255)                                                               tgTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTc                                                            >  1:47120/1‑140 (MQ=255)                                                               tgTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTc                                                            >  2:726270/1‑140 (MQ=255)                                                                gTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCt                                                           <  2:738129/140‑1 (MQ=255)                                                                   aaTAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGc                                                        >  2:94825/1‑140 (MQ=255)                                                                       ggCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCtttt                                                    <  2:400394/140‑1 (MQ=255)                                                                       ggCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCAAATAAGTTAAAGTTCCAGTTTTAGTGATGTTCTGGCtttt                                                    >  2:207925/1‑140 (MQ=255)                                                                           aTGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGt                                                >  2:445609/1‑140 (MQ=255)                                                                                  aTGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGAtt                                         >  1:598529/1‑140 (MQ=255)                                                                                        tGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAagag                                   >  2:443434/1‑140 (MQ=255)                                                                                              tAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCa                             <  1:94825/140‑1 (MQ=255)                                                                                                    aaTAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACt                       <  1:443434/140‑1 (MQ=255)                                                                                                         gTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTAccc                  >  2:412242/1‑140 (MQ=255)                                                                                                           gATGGCGACAGCAGAAAGTGAT‑GTGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCAc                >  1:660077/1‑140 (MQ=255)                                                                                                           gATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGATTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCAc                >  1:611968/1‑140 (MQ=255)                                                                                                            aTGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACt               >  1:656151/1‑140 (MQ=255)                                                                                                            aTGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACt               <  1:25714/140‑1 (MQ=255)                                                                                                                   cagcagAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCAttt        >  2:374563/1‑140 (MQ=255)                                                                                                                   cagcagAAAGTGAT‑‑GGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCATTTCCGTTGAGAGTCATGAGCTTTCTGATAGTTACCCACTTTCATTTc       >  2:186252/1‑140 (MQ=255)                                                                                                                       agAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCAt    <  2:602890/140‑1 (MQ=255)                                                                                                                         aaaGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCg  <  1:444146/140‑1 (MQ=255)                                                                                                                         aaaGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCg  <  1:18701/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                     GCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCG  >  NC_000913/1419587‑1419847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑