Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,544,042	(T)5→4	coding (19/1389 nt)	narU ←	nitrate/nitrite transporter NarU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,544,038	0	T	.	100.0%	181.3 / NA	43	coding (23/1389 nt)	narU	nitrate/nitrite transporter NarU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/24);  total (19/24)

GCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAAGA  >  NC_000913/1543899‑1544172                                                                                                                                            |                                                                                                                                       gcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCGAGAAGGCCTGATTTTCTGGTTTCCAGTCGCTCAAAAGATAACGACTAt                                                                                                                                        >  2:269230/1‑140 (MQ=255)          cATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCAtt                                                                                                                              >  2:401531/1‑140 (MQ=255)          cATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGGTTCCAGTAGCGGAAGAGATAACGACTA‑TTTTCTCAtt                                                                                                                              >  1:220317/1‑140 (MQ=255)            tCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCAtttt                                                                                                                            >  2:482548/1‑140 (MQ=255)             ccAGACACAGAAGGCAAGAAGTCGACAACTGGCTGGTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTg                                                                                                                           <  2:270904/140‑1 (MQ=255)             ccAGACACAGAAGGCAAGAAGTCGACAACTGACTGGTATGCAGAGATTGCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTg                                                                                                                           <  2:220317/140‑1 (MQ=255)             ccAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTg                                                                                                                           <  1:401531/140‑1 (MQ=255)              cAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGc                                                                                                                          <  1:672563/140‑1 (MQ=255)               aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCa                                                                                                                         >  1:734448/1‑140 (MQ=255)               aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAACATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCa                                                                                                                         <  2:142052/140‑1 (MQ=255)                gACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAg                                                                                                                        <  2:583544/140‑1 (MQ=255)                 acacAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGt                                                                                                                       >  2:455713/1‑140 (MQ=255)                  cacaGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTg                                                                                                                      <  2:20154/140‑1 (MQ=255)                  cacaGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTg                                                                                                                      <  2:11123/140‑1 (MQ=255)                    caGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGcc                                                                                                                    >  2:2102/1‑140 (MQ=255)                       aaGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCata                                                                                                                 >  2:21436/1‑140 (MQ=255)                       aaGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCata                                                                                                                 >  2:709311/1‑140 (MQ=255)                        aGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCatat                                                                                                                >  2:499040/1‑140 (MQ=255)                        aGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCatat                                                                                                                >  1:608650/1‑140 (MQ=255)                             agaagTAGACAACTGTCTGATAGCCAGAGCTTTCTTCCAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                           <  1:207337/140‑1 (MQ=255)                               aagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCt                                                                                                         >  2:156624/1‑140 (MQ=255)                               aagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCt                                                                                                         <  1:499040/140‑1 (MQ=255)                               aagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCt                                                                                                         >  1:431552/1‑140 (MQ=255)                                 gTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                                       <  2:431552/140‑1 (MQ=255)                                    gACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACAt                                                                                                    >  2:628245/1‑140 (MQ=255)                                    gACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACAt                                                                                                    <  1:2102/140‑1 (MQ=255)                                     aCAACTGACTGATATTCAGAGAGTTTTTCCAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATg                                                                                                   <  1:269230/140‑1 (MQ=255)                                     aCAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATg                                                                                                   <  1:736280/140‑1 (MQ=255)                                         ctgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcaca                                                                                               >  2:694070/1‑140 (MQ=255)                                                 tatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTGGCAGTGCTATAGTGTTCCTCAAATGCACACATTGGTa                                                                                       >  1:352098/1‑140 (MQ=255)                                                          aTTTCTTCGAGCAAGATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaa                                                                              >  2:457551/1‑140 (MQ=255)                                                               ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACa                                                                         <  2:569964/140‑1 (MQ=255)                                                                tcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaa                                                                        <  1:455713/140‑1 (MQ=255)                                                                 cGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaaa                                                                       <  1:21436/140‑1 (MQ=255)                                                                           gTTTTCCTTTATTTTACCAGAACGCTGGAGTTTCTGGCTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTa                                                             <  1:339773/140‑1 (MQ=255)                                                                                               aaGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatt                                         >  1:618594/1‑140 (MQ=255)                                                                                                         tttCTGGTTTCCAGTCGCGCAACAGCTAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAgg                               <  1:193509/140‑1 (MQ=255)                                                                                                         tttCTGGTTTCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAgg                               <  1:487582/140‑1 (MQ=255)                                                                                                                 ttCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTg                       >  2:125592/1‑140 (MQ=255)                                                                                                                  tCCAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTgg                      <  1:457551/140‑1 (MQ=255)                                                                                                                    cAGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGt                    <  1:694070/140‑1 (MQ=255)                                                                                                                     aGTCGCGCAAAAGATAACGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTg                   <  2:734448/140‑1 (MQ=255)                                                                                                                                    aaCGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGaaa    >  1:442231/1‑140 (MQ=255)                                                                                                                                      cGACTA‑TTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAAGa  <  1:724553/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                       GCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGCAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAAGA  >  NC_000913/1543899‑1544172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑