| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,555,655 | (T)7→8 | coding (15/1698 nt) | maeA ← | malate dehydrogenase, NAD‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,555,648 | 1 | . | T | 94.1% | 167.0 / 2.7 | 51 | coding (22/1698 nt) | maeA | malate dehydrogenase, NAD‑requiring |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base T (24/24); total (24/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
CCACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑G‑TTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGC > NC_000913/1555522‑1555780 | ccACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTAGGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTttt‑t‑tgtttt < 2:301518/140‑1 (MQ=255)ccACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTttt‑t‑tgtttt < 2:734504/140‑1 (MQ=255)ccACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTttt‑t‑tgtttt < 1:160027/140‑1 (MQ=255) aGTAACCCCAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGt < 2:59040/140‑1 (MQ=255) aaCCCCAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGtcac > 2:32093/1‑140 (MQ=255) tGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTg > 1:1883/1‑140 (MQ=255) gAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGa < 1:499852/140‑1 (MQ=255) gAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGa < 2:178261/140‑1 (MQ=255) cGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATatcca > 2:582022/1‑140 (MQ=255) tcCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTgggg < 1:652806/140‑1 (MQ=255) cTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCttt > 2:35951/1‑140 (MQ=255) tGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCtttt < 2:683681/140‑1 (MQ=255) gAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTa < 2:1883/140‑1 (MQ=255) tGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCttt > 1:720908/1‑140 (MQ=255) acaacGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCGTCCCTGGGGGGGTTGTATCGTCTTTGCTTTACCGCCa > 1:228213/1‑140 (MQ=255) acaacGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGGTTATATCGTGTTTGCTTTACCGCCa > 1:299260/1‑140 (MQ=255) acaacGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCa > 2:121816/1‑140 (MQ=255) acaacGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCa > 2:52407/1‑140 (MQ=255) cGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGGATATCCATTCCTGGGGGCTTTTTATAGTCTAAGCTTTACGGCCAGGGc > 2:355932/1‑140 (MQ=255) gacagttCCAGCAGTACCGGGCCAGCGTAAGGGATTTAATGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCg < 2:301324/135‑1 (MQ=255) ttCCAGCAGTACAGGTCCAGCGTAACGGATATAAAGCTAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCCCTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGc < 1:310158/140‑1 (MQ=255) ttCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGc < 2:452549/140‑1 (MQ=255) ttCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGc < 1:121816/140‑1 (MQ=255) ttCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGc < 2:579298/140‑1 (MQ=255) ttCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGc < 2:272317/140‑1 (MQ=255) cagcagTACAGGGCCTGCGTAAGGGCTATAAAGCGAAGGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTc < 1:355932/140‑1 (MQ=255) cagcagTACAGGGCCAGCGTAAGGGATATAACGCGCACGCT‑G‑TTTTT‑TCTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTc < 2:299260/140‑1 (MQ=255) cagcagTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTc < 1:582022/140‑1 (MQ=255) cagTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAg > 2:125480/1‑140 (MQ=255) tACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGtat > 1:387384/1‑140 (MQ=255) cAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACTGCCAGGGCGTCGGCCTCAAgctgtc > 1:301324/1‑135 (MQ=255) cAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGc > 1:593075/1‑129 (MQ=255) cAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGc > 1:573031/1‑129 (MQ=255) cAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGc < 2:593075/129‑1 (MQ=255) cAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGc < 2:573031/129‑1 (MQ=255) cAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATaa > 2:621684/1‑140 (MQ=255) ccAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTc < 1:561115/127‑1 (MQ=255) ccAGCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTc > 2:561115/1‑127 (MQ=255) gCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTc > 2:728472/1‑124 (MQ=255) gCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTc < 1:728472/124‑1 (MQ=255) gCGTAAGGGATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATa > 2:520326/1‑140 (MQ=255) tAAGGGATATAAAGCGAACGCTCT‑TTTTT‑T‑TGTTTCTGGTTCCATGTCGCTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATaaaa < 2:196604/140‑1 (MQ=255) ggATATAAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCAAGGGCGTCGGCGTCTAGTATAAAGCAGATaaaaacaa > 1:196604/1‑140 (MQ=255) tataAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATaaaaacaaaaa < 2:27337/140‑1 (MQ=255) taAAGCGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAaaaacaaaaaca < 1:520326/140‑1 (MQ=255) gCGAACGCT‑G‑TTTTTCT‑TGTTTATGTTTCCATGTCACCCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCAt < 1:292907/140‑1 (MQ=255) cGAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCAtt > 1:173603/1‑140 (MQ=255) gAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTg > 1:151604/1‑140 (MQ=255) gAACGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCAAGGGCGTCGGCCACAAGTATAAAGCCGATAAAAACAAAAACACCATTg > 2:292907/1‑140 (MQ=255) aaCGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTgc < 1:52407/140‑1 (MQ=255) aaCGCT‑GTTTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTgc > 1:168541/1‑140 (MQ=255) | CCACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCT‑G‑TTTTT‑T‑TGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGC > NC_000913/1555522‑1555780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |