| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,607,262 | (A)6→7 | coding (264/291 nt) | lsrG → | (4S)‑4‑hydroxy‑5‑phosphonooxypentane‑2,3‑dione isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,607,256 | 1 | . | A | 94.3% | 113.9 / ‑0.4 | 35 | coding (258/291 nt) | lsrG | (4S)‑4‑hydroxy‑5‑phosphonooxypentane‑2,3‑dione isomerase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (17/16); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGA > NC_000913/1607122‑1607389 | cAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑aa‑aa‑a < 1:431711/140‑1 (MQ=255) cccGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAACCTGGAATGTTTTATTACCGGGTCGCGT‑aa‑aa‑aaacgt > 1:213520/1‑136 (MQ=255) cccGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑aa‑aa‑aaacgt > 1:379760/1‑136 (MQ=255) cccGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCAGTGTCGCGAAACGGTAATCTTTAATGACCGGGCCGCGT‑aa‑aa‑aaacgt > 2:314698/1‑136 (MQ=255) ggAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTg < 1:591330/140‑1 (MQ=255) ggAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑aa‑aa‑aaacgt > 2:381776/1‑133 (MQ=255) ggAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑aa‑aa‑aaacgt < 1:381776/137‑5 (MQ=255) tGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCaa > 2:578889/1‑140 (MQ=255) aaTTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTGTAATGACGGGGGCGGGT‑AA‑AAGAACGTCTGTTCAATg > 1:219345/1‑140 (MQ=255) gcgcTTTTATATCTACGCAGCCCATAAAGATGAAGGCGCCGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTg < 2:219345/140‑1 (MQ=255) aCGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGa > 2:696613/1‑140 (MQ=255) cGAAGCCTATAAGGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGaa < 2:565460/140‑1 (MQ=255) gAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGAt > 1:60975/1‑126 (MQ=255) gAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGAt < 2:60975/126‑1 (MQ=255) taAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAAt > 2:40505/1‑140 (MQ=255) aGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAAtttt < 2:184903/140‑1 (MQ=255) tGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATc > 2:421926/1‑140 (MQ=255) tGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATc > 2:82780/1‑140 (MQ=255) tGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATc > 1:160457/1‑140 (MQ=255) tGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATc < 1:40505/140‑1 (MQ=255) gAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCt > 1:5509/1‑140 (MQ=255) agacaggCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCt < 1:634307/134‑1 (MQ=255) aaGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTa > 1:30498/1‑140 (MQ=255) aGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTAc > 2:410331/1‑140 (MQ=255) aGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTgg > 2:587873/1‑140 (MQ=255) tCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAAcgc > 1:23233/1‑140 (MQ=255) aCCACGCCCCACTACAACACCTCTGTCGCGACACTCGAAGCTTTAATGCCCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACgg < 2:213520/140‑1 (MQ=255) aCCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACgg < 2:379760/140‑1 (MQ=255) cGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGt > 1:643769/1‑140 (MQ=255) cGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGt > 1:600614/1‑140 (MQ=255) cGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATATACAATTGGGGTACCGCGCTGACGGGAGt > 1:300385/1‑140 (MQ=255) cAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGt < 2:679536/140‑1 (MQ=255) cAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGt < 1:696613/140‑1 (MQ=255) aaaCCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCt < 1:587873/140‑1 (MQ=255) tCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAAc < 1:421926/140‑1 (MQ=255) gcgAAACTGGAATCTTTTATGACCGGGCCGCGT‑AATAA‑ACCGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAAccc < 2:300385/140‑1 (MQ=255) gcgAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAAccc < 1:82780/140‑1 (MQ=255) cgAAACTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAAcccc > 2:451374/1‑140 (MQ=255) aCTGGAATCTTTAATGACCGGGCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCtt < 2:27439/140‑1 (MQ=255) gggCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGc > 1:627943/1‑140 (MQ=255) gggCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGc > 1:166287/1‑140 (MQ=255) ggCCGCGTAAA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCt < 2:30498/140‑1 (MQ=255) ccGCGTAAA‑AA‑AACGTCTGTTCCATCGTTTGATGCCGAGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGa < 1:314698/140‑1 (MQ=255) | CAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGT‑AA‑AA‑AACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGA > NC_000913/1607122‑1607389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |