| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,675,608 | (C)5→6 | coding (753/1389 nt) | pntB ← | pyridine nucleotide transhydrogenase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,675,603 | 1 | . | C | 97.0% | 110.2 / NA | 34 | A253G (GCT→GGT) | pntB | pyridine nucleotide transhydrogenase subunit beta |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base C (15/17); minor base A (1/0); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAG‑CCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTTCAGCATCGACACCACCACTGGCATATCTGCACCACCGATG > NC_000913/1675466‑1675737 | tctcTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAG‑cc < 2:707215/140‑1 (MQ=255) ggTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAAGGGTTCATCGCCTGACAAATAATGTAGGAAAAGATAGCCCCCC‑gaagaa > 2:209596/1‑140 (MQ=255) tGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTCCACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAAcc < 2:486676/140‑1 (MQ=255) aTTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGa > 2:170690/1‑140 (MQ=255) tttCGCGGTGCTCACCCACTTCCTGATCAGCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGAc < 1:356074/140‑1 (MQ=255) gCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTGCACATAATGTAAGAAAGGATAGCCCCCC‑GAGAAACCGACAAGCGCAcc > 2:356074/1‑140 (MQ=255) gCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCAcc > 1:149518/1‑140 (MQ=255) tCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATca > 2:642147/1‑140 (MQ=255) ccTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATcag < 2:505030/140‑1 (MQ=255) ccTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGACCCCC‑GAAGAAGCGGCCAGCGAACCGGTCACAATcag > 1:209207/1‑140 (MQ=255) atcatcGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGAGCACCGGTCACAATCAGCAgg < 1:170690/140‑1 (MQ=255) atcGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGGTAG‑CCCCCAGAAAAACAGACCAGCGTACCGTTCACAATCAGCAGGTCg > 2:240634/1‑140 (MQ=255) tcGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGt > 1:540035/1‑140 (MQ=255) tcGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGt > 1:637466/1‑140 (MQ=255) gCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCg < 1:384527/137‑1 (MQ=255) gCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCg > 2:384527/1‑137 (MQ=255) aGTAGAAGAGCCGTCCGTGCCGAAACCACACGCAATATCGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTg < 1:209596/140‑1 (MQ=255) cGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGcc > 2:509963/1‑140 (MQ=255) gTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGccc > 1:506122/1‑140 (MQ=255) gTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGccc > 2:106081/1‑140 (MQ=255) accCGCAATAACGCTCATAAAGCAACGGTTCATCGCCATACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAgccgcc < 1:240634/140‑1 (MQ=255) aTAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCgg < 1:106081/140‑1 (MQ=255) gAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCTGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTTCAGCa < 1:642147/140‑1 (MQ=255) gAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTTCAGCa < 2:149518/140‑1 (MQ=255) gAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTTCAGCa < 2:540035/140‑1 (MQ=255) aCGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAATAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAACCGCCCAGCCGGAGTACGAGTTCAGCATc < 2:611558/140‑1 (MQ=255) ttCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTTCAGCATCGaca < 2:521033/140‑1 (MQ=255) tCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTTCAGCATCGacac < 1:70203/140‑1 (MQ=255) cATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTTCAGCATCGAcacc < 2:506122/140‑1 (MQ=255) cGCCTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTTCAGCATCGAcaccacc < 1:509963/140‑1 (MQ=255) cTTACACATAATGTAAGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTTCAGCATCGACACCACCACt < 2:39245/140‑1 (MQ=255) aGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTTCAGCATCGACACCACCACTGGCATATCTGcacca > 1:160508/1‑140 (MQ=255) aGAAAGGATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTTCAGCATCGACACCACCACTGGCATATCTGcacca > 2:615666/1‑140 (MQ=255) gATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTTCAGCATCGACACCACCACTGGCATATCTGCACCACCGATg > 2:137918/1‑140 (MQ=255) gATAGCCCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAATACGAGTTAAGCATAGAGACCACCAATGGCTTATCTGCAACACCGATg > 2:350468/1‑140 (MQ=255) | TCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAG‑CCCCC‑GAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTTCAGCATCGACACCACCACTGGCATATCTGCACCACCGATG > NC_000913/1675466‑1675737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |