Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,818,550	(C)7→8	intergenic (‑50/+55)	chbF ← / ← chbR	monoacetylchitobiose‑6‑phosphate hydrolase/DNA‑binding transcriptional dual regulator ChbR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,818,543	1	.	C	100.0%	143.3 / NA	41	intergenic (‑43/+62)	chbF/chbR	monoacetylchitobiose‑6‑phosphate hydrolase/DNA‑binding transcriptional dual regulator ChbR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (24/17);  total (24/17)

GGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTAAACGTTTTA  >  NC_000913/1818406‑1818677                                                                                                                                           |                                                                                                                                         ggCAATTCGTGATAAAGCTTAACAATTCCCTCCAGTACCTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑c‑c                                                                                                                                      <  1:206376/140‑1 (MQ=255)     aTTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑c                                                                                                                                        <  1:619051/135‑1 (MQ=255)     aTTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑c                                                                                                                                        >  2:619051/1‑135 (MQ=255)      ttCGTGATAACGCTTCATAAATACTACCATTAACTCCCGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑c‑cccccc                                                                                                                                 <  1:245904/140‑1 (MQ=255)         gTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑c‑cccccccgg                                                                                                                              <  2:391816/140‑4 (MQ=255)          tGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTATCCCTTCAGTACGGAACACTGATATATGGCCTATCTG‑‑c‑cccccccgga                                                                                                                             >  2:206376/1‑136 (MQ=255)           gATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑c‑cccccccggac                                                                                                                            <  1:28473/140‑6 (MQ=255)                     aataaATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATc                                                                                                                   >  2:676420/1‑140 (MQ=255)                           tCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCaa                                                                                                             <  2:111580/140‑1 (MQ=255)                             cTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACa                                                                                                           <  1:16006/140‑1 (MQ=255)                               tCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAgg                                                                                                         >  2:364605/1‑140 (MQ=255)                               tCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAgg                                                                                                         >  2:68345/1‑140 (MQ=255)                               tCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAgg                                                                                                         >  1:10203/1‑140 (MQ=255)                               gccAGTAACTCCGGGGCATAACTGCTCCCGCCACCAATAGTGAAGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTAAAGAATACTGATACCTGACATATCTG‑‑CTCCACCCGTACATAAATACTCCAGCAACAga                                                                                                         >  2:537625/2‑139 (MQ=255)                                ccAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGa                                                                                                        <  1:68345/140‑1 (MQ=255)                                      aCTCCGGGGTATAGCCGCTCCCACCTCCATTAGTGACAACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGata                                                                                                  <  1:186163/140‑1 (MQ=255)                                       cTCCGGGGTATAGCTGGCCCCGTCACCAATAGTGACGACATTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGatat                                                                                                 <  2:334701/140‑1 (MQ=255)                                       cTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGatat                                                                                                 <  2:709558/140‑1 (MQ=255)                                       cTCCGGGGTATAGCTGCCCCCGCCACCAATAATGATGACCTCTAATTTTTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGatat                                                                                                 <  2:219494/140‑1 (MQ=255)                                            gggTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATGTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGaa                                                                                            <  2:173635/140‑1 (MQ=255)                                               tataGATGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTg                                                                                         <  1:537625/140‑1 (MQ=255)                                                ataGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGt                                                                                        <  1:364605/140‑1 (MQ=255)                                                    ctgctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCGGCAACAGAACAGAAATGTGAATTGTCAgg                                                                                    >  1:297504/1‑140 (MQ=255)                                                    ctgctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAgg                                                                                    >  1:585778/1‑140 (MQ=255)                                                       ctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATAtgtg                                                                                              >  1:411745/1‑127 (MQ=255)                                                       ctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATAtgtg                                                                                              <  2:411745/127‑1 (MQ=255)                                                       ctCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGtat                                                                                 >  2:623855/1‑140 (MQ=255)                                                         cccGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATaa                                                                               >  2:703961/1‑140 (MQ=255)                                                                ccaATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTa                                                                        <  1:623855/140‑1 (MQ=255)                                                                 caATAGTGACGACTTTTAATTTTTGGCTCATGATTTCTCTCTTCAGTATAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTAc                                                                       <  2:297504/140‑1 (MQ=255)                                                                 caATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTAc                                                                       <  1:676420/140‑1 (MQ=255)                                                                         acgacTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACTGATATGTGAATTGTCAGGTATAACGACTTACTGATTaaa                                                               >  2:298421/1‑140 (MQ=255)                                                                            acTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAAAAGGACAGAGATGGGAAGTGTCAGGTCTAACTACTTACTGATTAAATTc                                                            >  2:229801/1‑140 (MQ=255)                                                                                       cTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCGGGTATAAGGACGTATTGATTAAATTCAGTCAATTTCt                                                 >  1:354780/1‑140 (MQ=255)                                                                                             cATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCC‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGa                                            <  1:661930/140‑1 (MQ=255)                                                                                               tAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAg                                         >  1:129963/1‑140 (MQ=255)                                                                                                   ttCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGctct                                     <  2:10203/140‑1 (MQ=255)                                                                                                               tACAGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTATATTTAGTCAATTTCTTGCGATAGCTCTTAGGCGTAAAgg                         >  2:229321/1‑140 (MQ=255)                                                                                                                  aGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACg                      >  2:105063/1‑140 (MQ=255)                                                                                                                  aGAATACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACg                      >  2:511159/1‑140 (MQ=255)                                                                                                                    aaTACTGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGtt                    >  1:33849/1‑140 (MQ=255)                                                                                                                         tGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAAttt               >  1:76300/1‑140 (MQ=255)                                                                                                                         tGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGATCATAGGCGTAAAGGACGTTAAttt               >  1:341039/1‑140 (MQ=255)                                                                                                                         tGATATCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATCTCTTACGCTAGCTCATAGGCGTAAAGGACGTTAAttt               >  1:240933/1‑140 (MQ=255)                                                                                                                           atatCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCt             >  2:395670/1‑140 (MQ=255)                                                                                                                              tCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTaa          >  1:636470/1‑140 (MQ=255)                                                                                                                              tCTGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTaa          >  1:510009/1‑140 (MQ=255)                                                                                                                                tGGCATATCTGC‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTTAATTTCTTACGAGAACTTTTAGGCGTAAAGTCCGTTAATTTCTTAAAc        >  2:343174/1‑140 (MQ=255)                                                                                                                                      atCTGC‑C‑CCCCCCGGACGTAAAGAATCCAGCAACATGACTGATATGTGTATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTAAACGTTTta  <  2:240933/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         GGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑C‑CCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATTTCTTACGATAGCTCTTAGGCGTAAAGGACGTTAATTTCTTAAACGTTTTA  >  NC_000913/1818406‑1818677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑