| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,865,169 | (C)6→7 | coding (468/855 nt) | yeaE ← | methylglyoxal reductase YeaE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,865,163 | 1 | . | C | 97.2% | 123.2 / ‑3.3 | 36 | coding (474/855 nt) | yeaE | methylglyoxal reductase YeaE |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (14/21); total (14/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTT‑C‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACC > NC_000913/1865024‑1865296 | cATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGAttt > 1:66575/1‑140 (MQ=255) ttGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTT‑c‑c > 2:687370/1‑140 (MQ=255) gCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAg < 1:567989/140‑1 (MQ=255) ccTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCa > 1:38038/1‑140 (MQ=255) ggCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGt < 1:714678/140‑1 (MQ=255) cTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTc < 2:174044/140‑1 (MQ=255) aCGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTg < 2:66575/140‑1 (MQ=255) cGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGc > 1:715534/1‑140 (MQ=255) aCTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCata < 2:582167/140‑1 (MQ=255) tGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGGTTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATc > 2:510111/1‑140 (MQ=255) aaGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGTACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGc < 2:438784/140‑1 (MQ=255) cACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCa > 2:450307/1‑140 (MQ=255) ccGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAg > 1:548931/1‑140 (MQ=255) ccGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAg > 1:620543/1‑140 (MQ=255) ccGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTAATCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAg > 2:469496/1‑140 (MQ=255) tACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCACCATAATCAAGGTTAAAAACGCCCCAGCGGCGAATTTTTCCCTGGGCGa > 2:317250/1‑140 (MQ=255) tACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAACATAATCAAGGTTAAAAGCGCCCCAGCGGCGAATTTTTCACTGGGCGa > 2:356780/1‑140 (MQ=255) tCGTGAACCGAGATGCTAAAGCCCCTGATCAGTGGCAAACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTcc < 1:189709/140‑1 (MQ=255) gAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCg > 1:40609/1‑140 (MQ=255) ggTAAAGCACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGt < 2:715534/140‑1 (MQ=255) cACCTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCa > 2:148166/1‑140 (MQ=255) cttaTTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACa > 2:467432/4‑128 (MQ=255) cTGATTAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACa < 1:467432/128‑1 (MQ=255) tAGTGGCACACTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGaa < 2:620543/140‑1 (MQ=255) acacTGATTTCC‑CCCC‑CTGCAGATGCCAGAGCTCCTGCATATCAGCATGATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCa < 2:296285/140‑1 (MQ=255) acacTGATTT‑C‑CCCCACGGCAGGTGTCAGAGTTCCTGGATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCa < 1:317250/140‑1 (MQ=255) cacTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCaaaa > 1:64138/1‑127 (MQ=255) cacTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCaaaa < 2:64138/127‑1 (MQ=255) cacTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAg > 1:670016/1‑140 (MQ=255) acTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGa < 1:507620/140‑1 (MQ=255) acTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGa < 2:548931/140‑1 (MQ=255) acTGATTT‑CACCCC‑CGTCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGGCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGa < 2:421088/140‑1 (MQ=255) cTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGAc < 2:38038/140‑1 (MQ=255) cTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGAc < 2:117415/140‑1 (MQ=255) cTGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGAc < 1:159387/140‑1 (MQ=255) tGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGAcc < 2:621833/140‑1 (MQ=255) tGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGAcc < 1:450307/140‑1 (MQ=255) tGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGAcc < 1:687370/140‑1 (MQ=255) tGATTTCC‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGAcc < 2:40609/140‑1 (MQ=255) | CATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAGCACCTGATTAGTGGCACACTGATTT‑C‑CCCC‑CGGCAGCTGCCAGAGTTCCTGCATATCAGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACC > NC_000913/1865024‑1865296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |