| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,430,323 | (A)7→8 | coding (441/489 nt) | cvpA ← | colicin V production protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,430,316 | 1 | . | A | 93.2% | 134.7 / 2.9 | 44 | coding (448/489 nt) | cvpA | colicin V production protein |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base A (19/22); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
TCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAA > NC_000913/2430183‑2430445 | tcatAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑aaaa‑aa > 1:171858/1‑140 (MQ=255) tCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCt > 2:645050/1‑140 (MQ=255) gTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGAGGAGCTTTTCAGGTACTC‑AAAAGAAA‑CATCTGATGATa > 1:203335/1‑140 (MQ=255) cGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCt > 2:680253/1‑127 (MQ=255) cGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCt < 1:680253/127‑1 (MQ=255) ggCATAACACCGGCGATGCCGACAATACCGCCCATCCGTCTTTTTCCTAGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGa < 2:213847/140‑1 (MQ=255) aaCACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGc < 1:421337/140‑1 (MQ=255) cGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGAt < 2:579763/140‑1 (MQ=255) cGGCGATACCGACAACACCGCACATTCGTCTTTTTCCACGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGAt < 1:202921/140‑1 (MQ=255) gCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCa > 2:616205/1‑140 (MQ=255) gCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCa > 2:45633/1‑140 (MQ=255) aCCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTTACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCtgt > 1:121029/1‑140 (MQ=255) gACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGAt < 1:466292/140‑1 (MQ=255) gACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGAt < 1:616205/140‑1 (MQ=255) aCCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTc > 2:48083/1‑140 (MQ=255) cacaTACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATTAAACTGAGTTGGGGAATCAGCTGTGATTTGCTCCAGt > 1:192538/1‑140 (MQ=255) cacaTACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGt > 1:179772/1‑140 (MQ=255) aTACGTCTTTTTCCTTGTTAAGGCAAATCCCAGAGCAATTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCtt < 1:251814/140‑1 (MQ=255) aCGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCg > 1:540569/1‑140 (MQ=255) aCGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTCATGATAAAACTGTATTCCGGGATCAGCTGTAATTTGCTCCAGTCTTCg > 2:251814/1‑140 (MQ=255) cTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCttt > 2:14060/1‑140 (MQ=255) tttCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCg > 1:103461/1‑140 (MQ=255) ttCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAA‑AAACCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGa > 2:95028/1‑140 (MQ=255) ccTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGaca < 1:732905/140‑1 (MQ=255) cTCGTTAAGCCACATCTCAGAGTACTTACGCCCTGAGCACGAAACTTGCCGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGacac < 2:192538/140‑1 (MQ=255) cTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGacac < 2:179772/140‑1 (MQ=255) cTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGacac < 1:645050/140‑1 (MQ=255) cGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACAccc < 2:540569/140‑1 (MQ=255) cGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACAccc > 2:492018/1‑140 (MQ=255) gTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGacac < 1:410866/137‑1 (MQ=255) gTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGacac > 2:410866/1‑137 (MQ=255) tctcAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGt < 1:425905/140‑1 (MQ=255) tctcAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGt < 2:103461/140‑1 (MQ=255) gagCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCgaga < 1:594310/140‑1 (MQ=255) gagCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAATCgaga < 1:612299/140‑1 (MQ=255) aCTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGagaaaga > 1:418003/1‑140 (MQ=255) aCTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGagaaaga > 1:459558/1‑140 (MQ=255) ttACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAaagaag > 2:609419/1‑140 (MQ=255) tACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAagaaga > 2:693703/1‑140 (MQ=255) aCGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTATTCTACACCCCGGTAAACGAGTCGAGAAAGAagag > 2:239871/1‑140 (MQ=255) cGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAgaga < 2:418003/140‑1 (MQ=255) aGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGcaac < 1:559009/140‑1 (MQ=255) aGAAACTTGACGAGCTTTGCAGATAATCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGcaac < 1:14060/140‑1 (MQ=255) aGAAACTTGACGAGCATTGCAGTTACTCAAAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGcaac < 1:239871/140‑1 (MQ=255) gCAGATAATC‑AAAA‑AAACCATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCaa < 1:95028/140‑1 (MQ=255) | TCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATC‑AAAA‑AAA‑CATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAA > NC_000913/2430183‑2430445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |