| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,737,713 | (G)6→7 | intergenic (+67/‑32) | pheL → / → pheA | phe operon leader peptide/fused chorismate mutase/prephenate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,737,707 | 1 | . | G | 93.2% | 145.1 / 3.0 | 44 | intergenic (+61/‑38) | pheL/pheA | phe operon leader peptide/fused chorismate mutase/prephenate dehydratase |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (26/15); total (28/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑GGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGC > NC_000913/2737571‑2737839 | tgtactAAAGTCACTTACGGAATCAAACATGCAACTCATCCCGTTTTTGTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑ggg < 2:201819/140‑1 (MQ=255) actAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑ggg‑ggg < 1:151447/140‑1 (MQ=255) actAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑ggg‑ggg < 2:20010/140‑1 (MQ=255) tAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGATTGCCAGGGTGAACAATAAGGCCCCCCAAATA‑ggg‑ggggc > 2:341236/1‑138 (MQ=255) gTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑GGG‑GGGGCcttt > 2:647182/1‑140 (MQ=255) tCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑GGG‑GGGGCctttt > 1:578033/1‑140 (MQ=255) aCTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCtttttt > 1:100353/1‑140 (MQ=255) aCTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCtttttt > 2:25512/1‑140 (MQ=255) ggAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGAt < 1:488375/140‑1 (MQ=255) acaaacaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACa > 2:430821/1‑140 (MQ=255) acaaacaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACa > 2:594828/1‑140 (MQ=255) acaaacaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTATCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACa > 2:646879/1‑140 (MQ=255) tGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTTAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGc < 2:684929/140‑1 (MQ=255) tGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTTAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGc > 1:684929/1‑140 (MQ=255) tGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGc < 1:594828/140‑1 (MQ=255) aaaCACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCaa < 1:405733/140‑1 (MQ=255) tACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATg > 2:714346/1‑140 (MQ=255) tcttcGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGaa > 2:659092/1‑140 (MQ=255) ttcGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGaaaa < 1:430821/140‑1 (MQ=255) ttcGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTATCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGaaaa < 1:646879/140‑1 (MQ=255) cGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAGACAGAATGCGAAGACGAACAATAACGCCTCCCAAATCGGGG‑GGGCCTTTTTTACTGATAACAAAACGGAAACACTCTGACATCGGAAAAcc > 1:325578/1‑140 (MQ=255) cGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAAcc > 2:156899/1‑140 (MQ=255) gAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGaaa > 1:703856/1‑140 (MQ=255) aaTGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGaaaa > 2:524965/1‑140 (MQ=255) aaTGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGaaaa > 2:675178/1‑140 (MQ=255) gAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAATACTATGACATCGAAAATCCCGTTACTGGCGATGCGAGAGAAAATCAgc > 2:219584/1‑140 (MQ=255) gAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgc > 2:651620/1‑140 (MQ=255) aGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcg > 1:420183/1‑140 (MQ=255) gCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcgcg < 1:714346/140‑1 (MQ=255) gCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcgcg < 1:675178/140‑1 (MQ=255) gCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcgcg < 1:651620/140‑1 (MQ=255) gTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAGGGCAACACTAGGACATCGGCAAACCCGATACTGGCGATGCGAGAGAAAATCAGCGCGCt > 2:283640/1‑140 (MQ=255) gTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcgcg > 1:557228/1‑138 (MQ=255) gTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcgcg < 2:557228/138‑1 (MQ=255) gTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCt > 1:401398/1‑140 (MQ=255) gTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCt > 2:406933/1‑140 (MQ=255) tttCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTg > 1:686532/1‑140 (MQ=255) gtgtGAAACAGAATGCTAAGACGAACAATAAGGACTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGaaa < 2:312090/140‑1 (MQ=255) tgAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAat > 1:523522/1‑140 (MQ=255) aaTGCGAAGACGAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCg > 2:56720/1‑140 (MQ=255) cGAAGACGAACAATATGGCCTCGCACATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTAc < 1:341236/140‑1 (MQ=255) gAACAATAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGa < 2:100353/140‑1 (MQ=255) tAAGGCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGgcg > 2:182803/1‑140 (MQ=255) gCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGaa < 1:647182/140‑1 (MQ=255) gCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGaa < 1:406933/140‑1 (MQ=255) gCCTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGaa > 1:514012/1‑140 (MQ=255) gCCTCCCAAATC‑GGGTGGGCCTATTTTATTGATAACACAAGGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGaa < 1:283640/140‑1 (MQ=255) cTCCCAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACt < 2:523522/140‑1 (MQ=255) ccAAATCGGGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGc > 2:145825/1‑140 (MQ=255) | TGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑GGG‑GGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGC > NC_000913/2737571‑2737839 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |