| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,949,993 | (T)5→6 | coding (752/1332 nt) | argA → | N‑acetylglutamate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,949,988 | 1 | . | T | 97.8% | 153.0 / NA | 46 | R249R (CGC→CGT) | argA | N‑acetylglutamate synthase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base T (26/18); minor base G (0/1); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑TT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAG > NC_000913/2949863‑2950122 | ttttGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGgcg < 2:663304/140‑1 (MQ=255) tCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAg > 2:106197/1‑140 (MQ=255) ggCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGcc > 1:295434/1‑140 (MQ=255) gTCACTAATGACGACTGTGATATTGTCTCCGAACTTTCCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCa < 1:199098/140‑1 (MQ=255) gTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCa > 1:674839/1‑140 (MQ=255) gTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTACGTGGCGCAGTGAAAGCCTGCCGCa > 1:255955/1‑140 (MQ=255) cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAgc > 1:676787/1‑140 (MQ=255) cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTCAAAGCCTGCCGCAgc > 1:260501/1‑140 (MQ=255) aTGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgt > 1:63199/1‑140 (MQ=255) tGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtg < 1:384326/140‑1 (MQ=255) cgacgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtgcgt < 2:668605/140‑1 (MQ=255) ggTGATATTGTCTCCGAACTTTCCCCTAACGAAGCGCAAGCGCGGGTAAAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt < 2:253212/140‑1 (MQ=255) tctcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt > 1:686753/1‑140 (MQ=255) tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCg < 2:91538/126‑1 (MQ=255) tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCg > 1:91538/1‑126 (MQ=255) tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCa > 2:79062/1‑140 (MQ=255) tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCCTGGCGCAGTGAAGGCCTGCCGCAGAGGGGTGCGTAGCTGTCATTTAATCa > 1:210230/1‑140 (MQ=255) ccGAACTTTTCCCTAACAAAGCGCAAGCGCGTGTAAAAGGCCAGGAAGGGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAg < 2:247093/140‑1 (MQ=255) gAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGtt > 2:613777/1‑140 (MQ=255) aCTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTAt > 1:652711/1‑140 (MQ=255) ttCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAgg > 1:675915/1‑140 (MQ=255) gcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc < 1:106197/140‑1 (MQ=255) gcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGGGCGCTg > 2:706363/1‑140 (MQ=255) caagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGt < 2:63199/140‑1 (MQ=255) ggTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAagag < 1:613777/140‑1 (MQ=255) ggTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAagag > 1:362022/1‑140 (MQ=255) aGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGGTGTTGCAAGAGTTGTTCTCAcg > 2:289802/1‑140 (MQ=255) aGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTAAATCAGTTCTCACGAAAATGGCGCGATGTTTCAAGAGTTGTTCTCAcg > 2:230817/1‑140 (MQ=255) aaGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa < 2:652711/140‑1 (MQ=255) aaGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa < 2:675915/140‑1 (MQ=255) aaGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa < 2:674839/140‑1 (MQ=255) agaAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa > 1:34179/1‑137 (MQ=255) agaAAGGCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa < 2:34179/137‑1 (MQ=255) gCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGAc < 2:438155/132‑1 (MQ=255) gCGATTACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGAc > 1:438155/1‑132 (MQ=255) gATTACAACTCCGGTACGGTGCCCTTT‑TTTGCGTGGCGCCGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTa < 2:260501/140‑1 (MQ=255) ttACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACg > 1:117814/1‑140 (MQ=255) ttACAACTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGAGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACg > 2:421362/1‑140 (MQ=255) ttACAACTCCGGTACCGTGCGCGTT‑TTTGGGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACg < 1:230817/140‑1 (MQ=255) aCAACTCCGGTACGGTGCGC‑TTCTTTGCGTGACGCCGTGAAAGCCTGCTGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCa < 2:210230/140‑1 (MQ=255) aaCTCCGGTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa < 2:50247/140‑1 (MQ=255) ggTACGGTGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGTTGTTGCAATAGTGGGTATCACGCGACAGTAGCGGTACGCAGATtgtgt > 2:315529/1‑139 (MQ=255) tGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg > 1:379790/1‑140 (MQ=255) tGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCGCACGCGACGGTGTCGGTACGCGGATTGTGATGGAAAg > 1:223115/1‑140 (MQ=255) tGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGCCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa < 2:712668/127‑1 (MQ=255) tGCGCTTT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGCCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa > 1:712668/1‑127 (MQ=255) | TTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑TT‑TTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAG > NC_000913/2949863‑2950122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |