| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,606,215 | (G)8→10 | noncoding (72/87 nt) | leuP ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,606,207 | 1 | . | G | 94.1% | 50.8 / ‑3.0 | 17 | noncoding (80/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (7/9); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 4,606,207 | 2 | . | G | 88.2% | 47.0 / 0.7 | 17 | noncoding (80/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (7/8); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA > NC_000913/4606072‑4606322 || aGATAATTGGTGCGAGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑‑gg‑gg < 2:739297/140‑1 (MQ=255) aaTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCGCAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑‑gg‑ggg‑gg < 2:117139/139‑1 (MQ=255) aaTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑‑gg‑ggg‑gg > 1:117139/1‑139 (MQ=255) gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCAc > 1:15676/1‑140 (MQ=255) gACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑‑GG‑GGG‑GGGACTTGAACCCCCACGTc < 1:618328/140‑1 (MQ=255) gAACCCCCGCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑‑gg‑ggg‑gggggactt < 2:408589/123‑7 (MQ=255) gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGGGAGGGGG‑GGG‑GGGACTTGAACCCCCACGTCCGt < 2:722051/140‑1 (MQ=25) gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑‑gg‑ggg‑gggggactt > 1:408589/1‑117 (MQ=255) aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAGAACACt < 1:722487/140‑1 (MQ=255) cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCC‑CCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAGAACACTaa < 1:665036/140‑1 (MQ=37) gTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAGAACACTaa < 2:15676/140‑1 (MQ=255) acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAg < 1:388185/122‑1 (MQ=255) acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAg > 2:388185/1‑122 (MQ=255) tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc > 1:584725/1‑140 (MQ=255) cacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg < 2:584725/140‑1 (MQ=255) aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa > 1:64791/1‑140 (MQ=255) aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa < 1:92338/140‑1 (MQ=255) cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg > 1:420718/1‑140 (MQ=255) ttCCGCCACCTTCGCGCAGTCAACTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc < 1:273752/140‑1 (MQ=21) tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTATCGCGTCTACCAAGTCCTCCACCTTCGCCCAGTGCGAGCAATATCAACg > 2:208057/1‑140 (MQ=21) aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg > 2:370374/1‑140 (MQ=255) tGATATCGCCTCGTTTGGTGCGC‑‑‑GGAGGGCGGGACTTTAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa < 1:208057/140‑1 (MQ=21) || AGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑‑GG‑GGG‑GGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA > NC_000913/4606072‑4606322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |