| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 735,986 | (A)6→7 | coding (337/570 nt) | ybfC → | uncharacterized protein YbfC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 735,980 | 1 | . | A | 97.9% | 158.7 / ‑3.5 | 47 | coding (331/570 nt) | ybfC | uncharacterized protein YbfC |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (19/27); total (20/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
GCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAG‑A‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTGGCAGTCCTTGATAATTCAATCATGGTAAAATATTTTAAAGATGA > NC_000913/735844‑736113 | gCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCACGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAG‑a‑aa > 1:472678/1‑140 (MQ=255) aaaCAGAACTGGGTACATGTAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATTAAAAGAAGGTTGCAGGGGTTGATAAATACAGCAG‑a‑aaaa > 2:273811/1‑140 (MQ=255) aaaCAGAACTGGGTACATGTAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAG‑a‑aaaa > 2:401074/1‑140 (MQ=255) aaaCAGAACTGGGTACATGTAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAG‑a‑aaaa > 2:620878/1‑140 (MQ=255) gTACATGTAAAAATACTAAATTCACTCCAAGCGGGGCAAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTtct < 1:355965/140‑1 (MQ=255) tACATGTAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTtctc > 1:699778/1‑140 (MQ=255) aTGTAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGTCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGc > 1:45881/1‑140 (MQ=255) aTGTAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAGTACTGCGGAA‑AAAAACGGCGTTTCTCGGc > 1:331432/1‑140 (MQ=255) aTGTAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGc > 2:138167/1‑140 (MQ=255) aTGTAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGc > 1:401237/1‑140 (MQ=255) gTAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGa > 2:39868/1‑140 (MQ=255) gTAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGa > 1:490672/1‑140 (MQ=255) tAAAAATAATAAATTCCGACCATGCGGGGCGGGCCCCTTGATTCGCGTAGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGAt < 2:331432/140‑1 (MQ=255) tAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGAt < 2:580882/140‑1 (MQ=255) tAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGAt < 2:401237/140‑1 (MQ=255) tAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGAt < 2:699778/140‑1 (MQ=255) tAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGAt < 1:654077/140‑1 (MQ=255) tAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCGTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGAt < 1:273811/140‑1 (MQ=255) aaaTAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCgg < 1:620878/140‑1 (MQ=255) aataaATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTa < 2:705045/140‑1 (MQ=255) taaATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGa < 2:621114/140‑1 (MQ=255) taaATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGa < 2:369425/140‑1 (MQ=255) taaATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGCTGATAAATGCAGTAG‑AGAAAAACGGAGTTTATCGGCGATCGGTTTAGa > 2:196636/1‑140 (MQ=255) aaTTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGTCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAg < 2:45881/140‑1 (MQ=255) ccAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATaa < 2:139310/140‑1 (MQ=255) agtggggCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTtatat < 1:82245/132‑1 (MQ=255) agtggggCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTtatat > 2:82245/4‑135 (MQ=255) ggCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGt < 2:38351/140‑1 (MQ=255) gCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGta > 2:99762/1‑140 (MQ=255) gCGAGACTCTTAATTCGCGTCGCGTCACTTCGCCTTATTGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGta < 1:328098/140‑1 (MQ=255) ttAATTCGCGTCGGGTCACTACGCCTTAATGAGAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGCTTCTCGGCCATCTGATAAGAAGTTATATAATGGCGGGTATCTATCaaa > 2:323843/1‑140 (MQ=255) aaTTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCaaaaa < 2:490672/140‑1 (MQ=255) tCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAag < 2:472678/140‑1 (MQ=255) aCGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCcagtt < 1:138167/140‑1 (MQ=255) aCGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCcagtt < 1:689690/140‑1 (MQ=255) ccTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGTGGGTATCAATCGAAGAAAGAGCAGTGATCcagttcag > 1:220777/1‑140 (MQ=255) cTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCcagttcagt > 1:655060/1‑140 (MQ=255) tGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGttgttg > 1:31146/1‑140 (MQ=255) gAAAATCATGCCATCCAAGGAACGTCGCAAGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTgg < 1:196636/140‑1 (MQ=255) gAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTgg < 1:99762/140‑1 (MQ=255) gAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTgg < 1:407784/140‑1 (MQ=255) aaTCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTgg < 1:515164/137‑1 (MQ=255) aaTCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTgg > 2:515164/1‑137 (MQ=255) aaTCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTGGCAg > 1:602659/1‑140 (MQ=255) aaTCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCAATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTGGCAg > 2:281512/1‑140 (MQ=255) aTCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTGGCAGt < 1:401074/140‑1 (MQ=255) tCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTGGCAGTc < 2:522204/140‑1 (MQ=255) tGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTGGCAGTCCTTGATAATTCAATCATGGt > 1:708005/1‑140 (MQ=255) tGCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTGGCAGTCCTTGATAATTCAATCATGGt > 1:669538/1‑140 (MQ=255) gCAGGAGTTGATAAATACAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTGGCAGTCCTTGATAATTCAATCATGGTa < 2:669538/140‑1 (MQ=255) aCAGCAGAA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTGGCAGTCCTTGATAATTCAATCATGGTAAAATATTTTAAAGATg > 2:379837/1‑140 (MQ=255) cagcagaA‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTGGCAGTCCTTGATAATTCAATCATGGTAAAATATTTTAAAGATga > 1:632413/1‑140 (MQ=255) | GCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACACCAAGCGGGGCGAGACTCTTAATTCGCGTCGGGTCACTACGCCTTAATGAAAATCATGCCATCAAAGGAACGTTGCAGGAGTTGATAAATACAGCAG‑A‑AAAAACGGCGTTTCTCGGCGATCGGTTTAGAAGTTATATAAGAGCGGGTATCTATCAAAAAAAGAGCAGTGATCCAGTTCAGTTGTTGGCAGTCCTTGATAATTCAATCATGGTAAAATATTTTAAAGATGA > NC_000913/735844‑736113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |