Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,011,593	(A)7→8	coding (1630/1908 nt)	uup →	ATP‑binding protein with possible role in replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,011,586	1	.	A	100.0%	69.7 / NA	23	V541V (GTG→GTA)	uup	ATP‑binding protein with possible role in replication
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (11/12);  total (11/12)

TCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTG‑AAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGA  >  NC_000913/1011451‑1011717                                                                                                                                         |                                                                                                                                      tCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTG‑aaa‑a                                                                                                                                  <  1:393128/140‑1 (MQ=255)     cAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATTATGATGCCCCGGGTCCGCAAGAGCAGTATGTGGCGCCCAAACAGCCCGCGGTG‑aaa‑aaaaa                                                                                                                              >  2:515146/1‑139 (MQ=255)           gTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGGGGGCAGCAAGAGCAGTATGTGGCGCTCCAACAGCCTGCGGGG‑aaa‑aaaaaacgaag                                                                                                                        >  2:393128/1‑133 (MQ=255)             cGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCgaagaa                                                                                                                      <  1:689560/140‑1 (MQ=255)                         tACAGAATGTTGGATGTTCGAAAGCCGCGGTAAAATTTGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGTAGCCGCCGCGGCa                                                                                                          <  1:213706/140‑1 (MQ=255)                           cAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCaaa                                                                                                        <  2:146067/140‑1 (MQ=255)                                tGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCACACAGCCTGGGGGG‑AAATAAAA‑CCGATAAAAGCGCCGCGGCAAAAGCAg                                                                                                   >  2:290707/1‑140 (MQ=255)                                tGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGGATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAg                                                                                                   >  2:21635/1‑140 (MQ=255)                                 gTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGa                                                                                                  <  1:372819/140‑1 (MQ=255)                                          tCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTaaa                                                                                         >  1:505312/1‑140 (MQ=255)                                                 cggcggTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGGGAAAA‑AAAA‑CCGAAAAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAAagcagc                                                                                  >  2:413672/1‑140 (MQ=255)                                                        aaaaTTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCa                                                                           <  2:554110/140‑1 (MQ=255)                                                            ttGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAACAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACt                                                                       <  1:408131/140‑1 (MQ=255)                                                               gTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAg                                                                    <  2:418672/140‑1 (MQ=255)                                                                         cggcggTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTg                                                          <  1:696176/140‑1 (MQ=255)                                                                            cggTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATTTGGCGCTCAAACAGCCTGCGGGGAAAA‑AAAA‑CCGAAAAAGCCGCCGCGGCAAAAACAGAAACTGTAAAACCCAGCAGGAGAAAACTAAGCTATAAATTGCAg                                                       >  2:696176/1‑140 (MQ=255)                                                                              gTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGGGAAAA‑AAAA‑CCGAAAAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAgcg                                                     >  2:479599/1‑140 (MQ=255)                                                                                  tCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGaa                                                 <  1:515146/140‑1 (MQ=255)                                                                                     tgatgCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGAACTg                                              >  2:161129/1‑140 (MQ=255)                                                                                         gCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGAACTGGagc                                          >  1:86413/1‑140 (MQ=255)                                                                                                             gTATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGaa                      >  1:468738/1‑140 (MQ=255)                                                                                                              tATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGCACTTGACCAGCTACCGCAATTGCTCGAAg                     >  1:249247/1‑140 (MQ=255)                                                                                                              tATGTGGCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAg                     >  1:680589/1‑140 (MQ=255)                                                                                                                   ggCGCTCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTg                >  1:36342/1‑140 (MQ=255)                                                                                                                        tCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGc           >  2:149030/1‑140 (MQ=255)                                                                                                                        tCAAACAGCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGc           >  2:161046/1‑140 (MQ=255)                                                                                                                               gCCTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTg    <  2:680589/140‑1 (MQ=255)                                                                                                                               gCCTGCGGTG‑AAA‑AAAATCCGAAGAAGCCGCGGCGCCAAGAGCAGAAAATGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTg    <  2:249247/140‑1 (MQ=255)                                                                                                                                 cTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGa  <  2:36342/140‑1 (MQ=255)                                                                                                                                 cTGCGGTGAAAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGa  <  1:21635/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCTGCGGTG‑AAA‑AAAA‑CCGAAGAAGCCGCCGCGGCAAAAGCAGAAACTGTAAAACGCAGCAGTAGCAAACTAAGCTATAAATTGCAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGA  >  NC_000913/1011451‑1011717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑