Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	178,095	(G)5→6	coding (368/801 nt)	btuF ←	vitamin B12 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	178,090	1	.	G	100.0%	110.3 / NA	31	W125R (TGG→CGG)	btuF	vitamin B12 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (20/11);  total (20/11)

GTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCA‑GGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCCACGCCAGGCAATC  >  NC_000913/177957‑178217                                                                                                                                       |                                                                                                                                gTAAATGGCGGATTACTGCCGATTTCCAGAAAAACACCTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCA‑gggggg                                                                                                                           <  1:209069/140‑2 (MQ=255)           gATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACg                                                                                                                  >  1:393630/1‑140 (MQ=255)                aTGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACg                                                                                                             <  1:592894/140‑1 (MQ=255)                                        tttttAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTg                                                                                     <  2:619133/140‑1 (MQ=255)                                            tAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGc                                                                                 >  2:374198/1‑140 (MQ=255)                                                  tATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTCACGCATTGGCAATTTGTTCAAAGCTTGTCGCATCGAc                                                                           >  1:355453/1‑140 (MQ=255)                                                   aTCAGCATATTGCGCTTTCAATCGCGCGTGCTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGAcc                                                                          <  1:326811/140‑1 (MQ=255)                                                              gcgcTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACttt                                                               >  1:612873/1‑140 (MQ=255)                                                                  tttCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGTTTGCCGAATCGACTCACATCACTTTTAtt                                                           >  1:239915/1‑140 (MQ=255)                                                                  tttCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTAtt                                                           >  2:364832/1‑140 (MQ=255)                                                                  tttCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCAACCCACATCACTTTTAtt                                                           >  1:738260/1‑140 (MQ=255)                                                                             cgTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGc                                                >  1:119269/1‑140 (MQ=255)                                                                               tACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCa                                              <  2:393630/140‑1 (MQ=255)                                                                                    aTCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTgg                                         >  1:690515/1‑140 (MQ=255)                                                                                     tCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGt                                        >  2:547927/1‑140 (MQ=255)                                                                                         gcagGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAAc                                    >  2:150582/1‑140 (MQ=255)                                                                                         gcagGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCAACATCACCTTTATTCCCAGCGAAGCCAGCTGGTCAAc                                    >  1:317646/1‑140 (MQ=255)                                                                                          cagGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAAcc                                   >  2:657672/1‑140 (MQ=255)                                                                                          cagGGATTGCGCGACTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAAcc                                   >  2:69735/1‑140 (MQ=255)                                                                                              gATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGTTTGTCTCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGcc                               >  1:421041/1‑140 (MQ=255)                                                                                              gATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCt                                  >  2:88189/1‑137 (MQ=255)                                                                                              gATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCt                                  <  1:88189/137‑1 (MQ=255)                                                                                              gATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGcc                               >  1:680644/1‑140 (MQ=255)                                                                                                 tGCGCGGCTTGTTCGGCCTTGTTTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCt                            <  1:234687/140‑1 (MQ=255)                                                                                                  gcgcGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTc                           <  2:680644/140‑1 (MQ=255)                                                                                                   cgcgGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTCTATTCCCAGCGTAGACAGCTGGTCAACCTGCCGCTCg                          >  1:295956/1‑140 (MQ=255)                                                                                                     cgGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGc                        >  1:431864/1‑140 (MQ=255)                                                                                                       gCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTGTTCGCAGGGAAGCCAGCTGGTAAACCTGCCGCTCGGCAt                      >  2:234687/1‑140 (MQ=255)                                                                                                            ttCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCt                 <  1:657672/140‑1 (MQ=255)                                                                                                             tCGGCCTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTc                <  1:547333/140‑1 (MQ=255)                                                                                                                  cTTGTCTGGTTGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCCACGc           <  1:69735/140‑1 (MQ=255)                                                                                                                           ttGCGGACTCCAGGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCCACGCCAGGCAATc  <  1:93831/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                GTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCA‑GGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCCACGCCAGGCAATC  >  NC_000913/177957‑178217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑