Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,840	C→T	A345V (GCC→GTC)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,840	0	C	T	100.0%	78.2 / NA	26	A345V (GCC→GTC)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/15);  total (11/15)

GCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCAC  >  NC_000913/46706‑46974                                                                                                                                       |                                                                                                                                       gCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCtttt                                                                                                                                   >  2:700951/1‑140 (MQ=255)                 gCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGGTATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCggg                                                                                                                   >  1:78816/1‑140 (MQ=255)                 gCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGGTCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCggg                                                                                                                   >  2:106750/1‑140 (MQ=255)                  ccGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGCTTTGGGGCGAATCCCGGGTATGGGGATCTGGCTGGTGGTGATGGCCTTTGCGGGGTATGGCTTTTTCTCTGGCGGGCCcggg                                                                                                                  >  2:552602/1‑139 (MQ=255)                      aCGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAAtt                                                                                                              <  1:515036/140‑1 (MQ=255)                           gTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAg                                                                                                         <  1:552602/140‑1 (MQ=255)                            tCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGGAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGt                                                                                                        >  2:676446/1‑140 (MQ=255)                                ttgttgATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGct                                                                                                    <  1:106750/140‑1 (MQ=255)                                ttgttgATTGGCAGCGTTGCCATGAAGACTCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGct                                                                                                    <  1:232116/140‑1 (MQ=255)                                 tgttgATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGctc                                                                                                   <  1:700951/140‑1 (MQ=255)                                      aTTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGGGTATGTCTTTTTCTCTGGGGGGGCGTGTAATTAGCAGTGGCTCTATcc                                                                                              >  1:228034/1‑140 (MQ=255)                                        tGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTa                                                                                            <  1:394829/140‑1 (MQ=255)                                                  gCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCtt                                                                                  <  2:63726/140‑1 (MQ=255)                                                    cATGATGACGCTGGCGCGGGCGCTTTTGGGGTTAAGCCCTGATATGGGCATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTcc                                                                                <  2:298372/140‑1 (MQ=255)                                                        atgaCGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGGAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGAc                                                                            >  1:595596/1‑140 (MQ=255)                                                               tggcgctggcgGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATc                                                                     <  2:620488/140‑1 (MQ=255)                                                                ggcgctggcgGTTTTGGGGCTAATCCCGGGTTTGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGGAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATcc                                                                    >  1:584652/1‑140 (MQ=255)                                                                 gcgctggcgGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCcg                                                                   <  2:594851/140‑1 (MQ=255)                                                                                 ggCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGc                                                   <  1:116218/140‑1 (MQ=255)                                                                                                tGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGCAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGTTATCCGCTCCTGTGACGTGGGGGTGATTATGTCCTTa                                    >  1:237601/1‑140 (MQ=255)                                                                                                  gggATATGGCTGGTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAg                                  >  2:104990/1‑140 (MQ=255)                                                                                                          gctggTAGTGATGGCCTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTg                          >  2:614856/1‑140 (MQ=255)                                                                                                                        ccTTTGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCg            >  1:115440/1‑140 (MQ=255)                                                                                                                            tGCGGTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCt        >  2:59108/1‑140 (MQ=255)                                                                                                                               ggTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTggg     <  1:614856/140‑1 (MQ=255)                                                                                                                               ggTGTATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTggg     <  2:584652/140‑1 (MQ=255)                                                                                                                                 tgtATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCa   <  1:676446/140‑1 (MQ=255)                                                                                                                                  gtATGTCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCAc  <  1:59108/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCAC  >  NC_000913/46706‑46974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑