Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,587,142	T→C	D198G (GAC→GGC)	ydeQ ←	putative fimbrial adhesin protein YdeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,587,142	0	T	C	100.0%	188.4 / NA	52	D198G (GAC→GGC)	ydeQ	putative fimbrial adhesin protein YdeQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/27);  total (25/27)

TTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACAT  >  NC_000913/1587011‑1587275                                                                                                                                    |                                                                                                                                      ttgttgCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGgaga                                                                                                                               <  1:59593/140‑1 (MQ=255)   gttgCATCAGGTGCTGTTTTAGTAAACACTTCTCGGCTACTGTCAGTGCTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATc                                                                                                                             <  2:195791/140‑1 (MQ=255)     tgCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCa                                                                                                                           <  2:513299/140‑1 (MQ=255)         tCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGt                                                                                                                       <  2:634009/140‑1 (MQ=255)                 tgtgTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTACGCTTCCTGGAAAGCCCGGGAGATCCACGGTGACATTTc                                                                                                               >  2:305397/1‑140 (MQ=255)                  gtgtTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCg                                                                                                              <  2:650837/140‑1 (MQ=255)                  gtgtTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCg                                                                                                              <  1:701255/140‑1 (MQ=255)                     ttAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCAGGCGATCCATGGTGACATTTCGACt                                                                                                           >  1:346980/1‑140 (MQ=255)                        gCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATc                                                                                                        >  2:625439/1‑140 (MQ=255)                                  gTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACt                                                                                                           <  2:475357/127‑1 (MQ=255)                                  gTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACt                                                                                                           >  1:475357/1‑127 (MQ=255)                                  gTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAg                                                                                              >  2:154069/1‑140 (MQ=255)                                   tCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAgc                                                                                             <  1:154069/140‑1 (MQ=255)                                        tACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCt                                                                                        >  2:484649/1‑140 (MQ=255)                                        tACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCt                                                                                        <  1:484649/140‑1 (MQ=255)                                         aCTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTg                                                                                       <  1:625439/140‑1 (MQ=255)                                          cTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATTCACCGTGACATTTCGAATATCGACGGTGCAGCCGCCTGt                                                                                      >  2:239872/1‑140 (MQ=255)                                             tCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAgc                                                                                             >  1:173497/1‑130 (MQ=255)                                             tCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAgc                                                                                             <  2:173497/130‑1 (MQ=255)                                               aGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCa                                                                                 <  2:544328/140‑1 (MQ=255)                                               aGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCa                                                                                 <  2:51912/140‑1 (MQ=255)                                                         cccGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGAGTATCAACCGTGCAGACGCCTGATGGCATAACAACAtt                                                                       >  1:252746/1‑140 (MQ=255)                                                               aGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTAtt                                                                 <  2:720921/140‑1 (MQ=255)                                                               aGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTAtt                                                                 <  1:473517/140‑1 (MQ=255)                                                                 aTAAAATGAGAGCTTTTGTTCGTTTGAGCGATATACGGCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTg                                                               <  1:242108/140‑1 (MQ=255)                                                                 aTAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTg                                                               <  1:59964/140‑1 (MQ=255)                                                                           agCTTTTGTTCGCTTAAGCAATCTACGCCTAGGGGTACTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATAtt                                                     <  1:219342/140‑1 (MQ=255)                                                                             cTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTcc                                                   <  1:619048/140‑1 (MQ=255)                                                                                ttGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCC‑‑‑‑‑ATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTaaaa                                           >  1:394008/1‑140 (MQ=255)                                                                                          gAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCTGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTAcg                                      <  2:346980/140‑1 (MQ=255)                                                                                          gAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTAcg                                      <  1:381495/140‑1 (MQ=255)                                                                                            gCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGCGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATGTGATATGATATTCTACGTAAAATTAcgcg                                    >  1:202767/1‑140 (MQ=255)                                                                                                  taCGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTc                              >  1:614459/1‑140 (MQ=255)                                                                                                  taCGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTc                              >  1:185920/1‑140 (MQ=255)                                                                                                     gCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTcc                             >  1:16735/1‑138 (MQ=255)                                                                                                     gCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTcc                             <  2:16735/138‑1 (MQ=255)                                                                                                     gCCAAGGGGTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGc                           >  1:500749/1‑140 (MQ=255)                                                                                                          ggggTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCGACGAAAGATTACGGGGATTTCCGCTTCCt                      >  1:214471/1‑140 (MQ=255)                                                                                                          ggggTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCt                      >  2:76998/1‑140 (MQ=255)                                                                                                          ggggTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCt                      >  2:431227/1‑140 (MQ=255)                                                                                                          ggggTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCt                      >  1:106595/1‑140 (MQ=255)                                                                                                          ggggTATTTCTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAAAAATATTCCACGTAAGATTACGAGGATTTCCGCTTCCt                      >  1:343981/1‑140 (MQ=255)                                                                                                                 ttCTGCGCTTCCAGGAACGCCCAGGAGATCCCCGGCGACCTTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTg               <  2:214471/140‑1 (MQ=255)                                                                                                                   cTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCa             >  2:728255/1‑140 (MQ=255)                                                                                                                   cTGCGCTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCa             >  1:45725/1‑140 (MQ=255)                                                                                                                     gcgcTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAAt           <  1:76998/140‑1 (MQ=255)                                                                                                                      cgcTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAAtt          >  2:78093/1‑140 (MQ=255)                                                                                                                      cgcTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAAtt          >  1:509749/1‑140 (MQ=255)                                                                                                                      cgcTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTAAGCGGATGTCCGCTGCCTAATGTTGCAAtt          >  1:208833/1‑140 (MQ=255)                                                                                                                       gcTTCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAAttt         >  2:476406/1‑140 (MQ=255)                                                                                                                         ttCCAGGAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTta       <  2:500749/140‑1 (MQ=255)                                                                                                                           ccAGGAAAGCCCGGGAGATCCATGGTGACATTTCGTCTATCAACGGTGAAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTtata     <  2:252746/140‑1 (MQ=255)                                                                                                                           ccAGGAAAGCCCGGGAGATCCACGGTGACATTACGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTtata     <  1:648373/140‑1 (MQ=255)                                                                                                                              ggAAAGCCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACat  <  1:476406/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      TTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACAT  >  NC_000913/1587011‑1587275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑