Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,603,815	T→C	V266A (GTA→GCA)	lsrC →	Autoinducer‑2 ABC transporter membrane subunit LsrC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,603,815	0	T	C	100.0%	70.0 / NA	22	V266A (GTA→GCA)	lsrC	Autoinducer‑2 ABC transporter membrane subunit LsrC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/12);  total (10/12)

TTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGC  >  NC_000913/1603686‑1603949                                                                                                                                  |                                                                                                                                       ttGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCa                                                                                                                              <  1:695969/140‑1 (MQ=255) ttGTGTTTGCTTCGCAGATTGGCTTTATCACCAACCACACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCa                                                                                                                              <  1:215752/140‑1 (MQ=255)         gCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCt                                                                                                                      >  2:650741/1‑140 (MQ=255)           ttCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGa                                                                                                                    <  1:581321/140‑1 (MQ=255)                     ggTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGa                                                                                                          <  1:650741/140‑1 (MQ=255)                            tCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCTCATGGTTCCTGACGCAGATCGATAGCGTa                                                                                                   >  1:334111/1‑140 (MQ=255)                            tCCCCAACAAGACCGGTACCGGGCTGGAGATGAGAGCAATTGCACCCTGCGGGCTGGGCGGCATTTTTTGGCTCGGTGTTTCCGGTGTGATCATTGGTGCGGCACTCGGCGGATGGTTCCTGACGCAGATCGGTAGCGTa                                                                                                   >  2:418359/1‑140 (MQ=255)                             ccccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTAc                                                                                                  >  1:30956/1‑140 (MQ=255)                                 aaCCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGt                                                                                              >  2:76004/1‑140 (MQ=255)                                         cGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTgc                                                                                      <  1:461806/140‑1 (MQ=255)                                          ggTACCGGGCTGGAGATGAAAGTAATTGCAGGCCGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTgcg                                                                                     <  2:334111/140‑1 (MQ=255)                                                 ggCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTgg                                                                                               <  1:606778/123‑1 (MQ=255)                                                 ggCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTgg                                                                                               >  2:606778/1‑123 (MQ=255)                                                    tGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCa                                                                           <  1:76004/140‑1 (MQ=255)                                                      gagaTGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGTTCGATAGAGTACTGCTGCTGTTGCGCATTCCGGCAtg                                                                         >  1:327728/1‑140 (MQ=255)                                                                           tGCGTGCTGGGCGGCATTAGTTCGCCCGGTGGTTCCGATGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCggg                                                    <  1:209055/140‑1 (MQ=255)                                                                                         cATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTgg                                      >  1:93564/1‑140 (MQ=255)                                                                                         cATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTgg                                      <  2:696094/140‑1 (MQ=255)                                                                                                   cTCGGTGGTTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTggg                            >  2:37365/1‑139 (MQ=255)                                                                                                         ggtTCCGGTGCGATCATTGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGa                      <  2:30956/140‑1 (MQ=255)                                                                                                                gTGCGATCATTGGTGGGGCACTCGGTGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGc               <  1:329730/140‑1 (MQ=255)                                                                                                                         ttGGTGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGGGTTTGATGGACGCCTGCGTtgt      >  2:635881/1‑140 (MQ=255)                                                                                                                             tGCGGCACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTgcgc  >  2:11403/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                       TTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGC  >  NC_000913/1603686‑1603949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑