BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	216,269	C→A	A245A (GCG→GCT)	yaeF ←	peptidase C92 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	216,269	0	C	A	100.0%	127.2 / NA	37	A245A (GCG→GCT)	yaeF	peptidase C92 family protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (20/17); total (20/17)

ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGAC  >  NC_000913/216139‑216395

aCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACa                                                                                                                       <  2:548158/140‑1 (MQ=255)

 ccccccTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACAt                                                                                                                      <  1:51637/140‑1 (MQ=255)

    cccTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCAc                                                                                                                   >  1:431477/1‑140 (MQ=255)

      cTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACCGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCa                                                                                                                 >  2:672227/1‑140 (MQ=255)

         aTACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCAACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACCGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATa                                                                                                              <  1:672227/140‑1 (MQ=255)

          tACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTACCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATAc                                                                                                             <  2:636952/140‑1 (MQ=255)

               tAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCata                                                                                                        >  1:182284/1‑140 (MQ=255)

               tAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCata                                                                                                        >  2:50072/1‑140 (MQ=255)

                       aTAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAg                                                                                                >  1:422509/1‑140 (MQ=255)

                            gCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCgg                                                                                           <  2:422509/140‑1 (MQ=255)

                             cATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGc                                                                                          <  2:431477/140‑1 (MQ=255)

                                      tATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAAt                                                                                 >  2:137711/1‑140 (MQ=255)

                                              ggTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCg                                                                         >  2:473441/1‑140 (MQ=255)

                                                   aaCCAACAAGGCGACGCGCCTTTATTTAATTCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTgc                                                                    <  1:359039/140‑1 (MQ=255)

                                                     ccAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTgcgc                                                                  <  2:684919/140‑1 (MQ=255)

                                                      caacaaAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTAGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGcc                                                                 <  1:137711/140‑1 (MQ=255)

                                                       aacaaAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCa                                                                <  1:473441/140‑1 (MQ=255)

                                                         caaAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGa                                                                        >  1:529557/1‑130 (MQ=255)

                                                         caaAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGa                                                                        <  2:529557/130‑1 (MQ=255)

                                                              cGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAg                                                         >  2:184816/1‑140 (MQ=255)

                                                              cGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCACATGCATCAGATCAGCATGGCTAATCGAGCCGGATTGCGCCAGTGTAAg                                                         >  2:224510/1‑140 (MQ=255)

                                                              cGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCAAATGCATCAAATCGGCATGGCTAATCTAGCCAGATTGCGCCAGTGTAAg                                                         >  2:299576/1‑140 (MQ=255)

                                                                  ccGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGa                                                     >  1:441298/1‑140 (MQ=255)

                                                                      cTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGcc                                                 >  2:434535/1‑140 (MQ=255)

                                                                           tataAATCCCAGGCTTCAGGTGACCTACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCt                                            <  1:224510/140‑1 (MQ=255)

                                                                              aaaTCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCTTTg                                         >  1:422526/1‑140 (MQ=255)

                                                                                aTCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGCGCTAATCCGGCCCGTTAGCGCCAGTGTAAGGAGATGCCCGGCTTTGGc                                       >  2:341089/1‑140 (MQ=255)

                                                                                   ccAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCTTTGGCaaa                                    <  2:182284/140‑1 (MQ=255)

                                                                                     aGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCTTTGGc                                       >  1:707856/1‑135 (MQ=255)

                                                                                     aGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCTTTGGc                                       <  2:707856/135‑1 (MQ=255)

                                                                                      ggCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCTTTGGCAAAGGc                                 <  1:690143/140‑1 (MQ=255)

                                                                                                      cATACTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTcc                 >  1:396818/1‑140 (MQ=255)

                                                                                                          cTGTAACTGCGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAAc             <  1:434535/140‑1 (MQ=255)

                                                                                                                   cGTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACg    >  2:177000/1‑140 (MQ=255)

                                                                                                                    gTTTCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGa   <  1:50072/140‑1 (MQ=255)

                                                                                                                     tttCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGAc  >  1:659888/1‑140 (MQ=255)

                                                                                                                     tttCTGGCTTAAAAGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGAc  >  2:78038/1‑140 (MQ=255)

ACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCAGAGACATCACCAATACGCATATGCATCAGATCGGCAGGGCTAATCCAGCCCGATTGCGCCAGTGTAAGTGGATGCCCGGCTTTGGCAAAGGCATCCGTGACAAATTCCGAACAAAACCACGAC  >  NC_000913/216139‑216395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`