Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,699,694	T→C	F114L (TTC→CTC)	malX →	PTS enzyme IIBC component MalX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,699,694	0	T	C	100.0%	109.1 / NA	31	F114L (TTC→CTC)	malX	PTS enzyme IIBC component MalX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/17);  total (14/17)

GCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTC  >  NC_000913/1699563‑1699828                                                                                                                                    |                                                                                                                                       gCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTg                                                                                                                                >  1:505574/1‑140 (MQ=255) gCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTg                                                                                                                                >  2:688670/1‑140 (MQ=255) gCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTg                                                                                                                                >  2:24858/1‑140 (MQ=255)  cTCGTTTGCTTTTAGCTTCCTGGCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGa                                                                                                                               <  1:272156/140‑1 (MQ=255)   tCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGAc                                                                                                                              >  2:486975/1‑140 (MQ=255)    cGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGAcc                                                                                                                             <  2:162479/140‑1 (MQ=255)    cGTTTGCTTTTAGTTACCTGCCAGTCATGGTCTGTATTGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGAcc                                                                                                                             <  1:341046/140‑1 (MQ=255)      gttGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCCCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCCGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCaa                                                                                                                           >  2:523149/2‑140 (MQ=255)         gCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATaa                                                                                                                        <  2:125854/140‑1 (MQ=255)                gTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCAtt                                                                                                                 <  1:486975/140‑1 (MQ=255)                   tCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTg                                                                                                              <  2:149345/140‑1 (MQ=255)                                      tATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCgg                                                                                           >  2:636914/1‑140 (MQ=255)                                      tATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCgg                                                                                           >  1:677090/1‑140 (MQ=255)                                      tATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCgg                                                                                           <  2:505574/140‑1 (MQ=255)                                      tATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCgg                                                                                           >  2:162361/1‑140 (MQ=255)                                              tCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGa                                                                                     <  1:104555/138‑1 (MQ=255)                                              tCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGa                                                                                     >  2:104555/1‑138 (MQ=255)                                                ccGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGc                                                                                 <  1:597162/140‑1 (MQ=255)                                                ccGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGc                                                                                 <  2:469407/140‑1 (MQ=255)                                                     gggCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATa                                                                            <  2:660340/140‑1 (MQ=255)                                                            gCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGTCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCa                                                                     >  2:313611/1‑140 (MQ=255)                                                            gCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCa                                                                     >  2:397405/1‑140 (MQ=255)                                                            gCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCa                                                                     <  1:636914/140‑1 (MQ=255)                                                                                aGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGt                                                 >  2:572536/1‑140 (MQ=255)                                                                                 gCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCGATAACATCCAGAGAATTCTTGGGATCCAGtc                                                >  2:311936/1‑140 (MQ=255)                                                                                         cGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATa                                        <  1:24858/140‑1 (MQ=255)                                                                                                ttCATCGGTTATGCGGTGATGACCCTCGCGGTAAAACTCGGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGAt                                 <  2:271040/140‑1 (MQ=255)                                                                                                   aTCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCTGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCt                              <  2:90488/140‑1 (MQ=255)                                                                                                   aTCGGTTATGCGGTAATGAACCTCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCt                              <  2:677090/140‑1 (MQ=255)                                                                                                                         tCGCGGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTTGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGtatt        >  1:649937/1‑140 (MQ=255)                                                                                                                          cgcgGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGtatta       >  2:509883/1‑140 (MQ=255)                                                                                                                          cgcgGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGtatta       >  2:558759/1‑140 (MQ=255)                                                                                                                          cgcgGTAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGtatta       >  1:606998/1‑140 (MQ=255)                                                                                                                               tAAACCTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTc  <  1:509883/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       GCTCGTTTGCTTTTAGTTTCCTGCCTGTCATGTTCTGTATCGCCATCCCGCTGGGCCTGGCACGCGAAAATAAAGGCGTAGCGGCATTCGCTGGCTTCATCGGTTATGCGGTAATGAACCTCGCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTC  >  NC_000913/1699563‑1699828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑