Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,709,945	A→G	A268A (GCA→GCG)	rsxD →	SoxR [2Fe‑2S] reducing system protein RsxD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,709,945	0	A	G	100.0%	110.5 / NA	32	A268A (GCA→GCG)	rsxD	SoxR [2Fe‑2S] reducing system protein RsxD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/14);  total (18/14)

CTCGCCTGGCTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCAGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTGGTCGTCTTATTTTCGGCGCGCTTGCGGGCTTAT  >  NC_000913/1709814‑1710069                                                                                                                                    |                                                                                                                             ctcGCCTGGCTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCa                                                                                                                      <  1:460620/140‑1 (MQ=255)              ctggcGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATctgctg                                                                                                         <  2:177176/140‑1 (MQ=255)                     cgTATGGTTGCTATGTCAGAAAACGCTTCCCTGGCATTTTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAg                                                                                                  <  1:223027/140‑1 (MQ=255)                              gCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGc                                                                                         <  1:649613/140‑1 (MQ=255)                               cTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCt                                                                                        <  1:109725/140‑1 (MQ=255)                                   ggCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGgc                                                                                    <  2:396414/140‑1 (MQ=255)                                      aGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCTCAAATTCATCTGCTGTTTGGAACGACCATGCTCGGCGCa                                                                                 >  2:275388/1‑140 (MQ=255)                                         aaGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTc                                                                              >  2:613124/1‑140 (MQ=255)                                                   cTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTtga                                                                    <  1:720289/140‑1 (MQ=255)                                                   cTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTtga                                                                    <  1:613124/140‑1 (MQ=255)                                                     ggCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTtgact                                                                  <  2:633344/140‑1 (MQ=255)                                                        atatTCCCCTCAGCTCCTTAGTTACCCTGTCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTtgactgac                                                               <  1:212926/140‑1 (MQ=255)                                                               ccTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTa                                                        <  1:110795/140‑1 (MQ=255)                                                                cTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTAc                                                       >  1:88593/1‑140 (MQ=255)                                                                                cGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAAt                                       >  2:568123/1‑140 (MQ=255)                                                                                   tGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTAATTTGACTAACCGGGCTACCGCGTCTACGACCAATCGt                                    >  1:208363/1‑140 (MQ=255)                                                                                     gCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTgg                                  >  2:462864/1‑140 (MQ=255)                                                                                     gCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTATGACTGACCCGGTTACCGCTTATACGACCAATCGTgg                                  >  1:326126/1‑140 (MQ=255)                                                                                           tGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTGGTCGTCt                            >  1:529123/1‑140 (MQ=255)                                                                                           tGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTGGTCGTCt                            >  1:438927/1‑140 (MQ=255)                                                                                               cAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTGGTCGTCTTAtt                        >  1:413993/1‑140 (MQ=255)                                                                                                  tGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTTACTGACCCAGTTACTGCTTCTACGAACAATCGTGGTCGTCTTATTTTc                     >  2:329127/1‑140 (MQ=255)                                                                                                  tGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTGGTCGTCTTATTTTc                     >  2:367525/1‑140 (MQ=255)                                                                                                           ggTTGTTCTCACCAGTAACACTTGCGGCATCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTGGTCGTCTTATTTTCGGCGCGCtt            <  1:329127/140‑1 (MQ=255)                                                                                                            gttgttCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCATTCGGGGGCGTCTTGTTTTCGGCGCGCTTg           >  1:203736/1‑140 (MQ=255)                                                                                                            gttgttCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCATTCGGGGCCGTCTTCTTTTCGGCGCGCTTg           >  1:272217/1‑140 (MQ=255)                                                                                                            gttgttCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTGGTCGTCTTATTTTCGGCGCGCTTg           >  1:458362/1‑140 (MQ=255)                                                                                                            gttgttCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTGGTCGTCTTATTTTCGGCGCGCTTg           >  2:392929/1‑140 (MQ=255)                                                                                                            gttgttCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTGGTCGTCTTATTTTCGGCGCGCTTg           >  1:558849/1‑140 (MQ=255)                                                                                                              tgttCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTGGTCGTCTTATTTTCGGCGCGCTTGCg         >  1:23988/1‑140 (MQ=255)                                                                                                               gttCTCACCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTGGTCGTCTTATTTTCGGCGCGCTTGCgg        <  2:438927/140‑1 (MQ=255)                                                                                                                     aCCAGAAACACTGGCGGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTGGTCGTCTTATTTTCGGCGCGCTTGCGGGCttat  <  2:88593/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                             CTCGCCTGGCTGGCTGGCGGCGTATGGTTGCTATGGCAGAAAGCGATTCGCTGGCATATTCCCCTCAGCTTCTTAGTAACGCTGGCGTTATGCGCAATGTTGGGCTGGTTGTTCTCACCAGAAACACTGGCAGCACCGCAAATTCATCTGCTGTCTGGAGCGACCATGCTCGGCGCATTCTTTATTTTGACTGACCCGGTTACCGCTTCTACGACCAATCGTGGTCGTCTTATTTTCGGCGCGCTTGCGGGCTTAT  >  NC_000913/1709814‑1710069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑