| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,754,383 | G→A | A32V (GCC→GTC) | ydhY ← | putative 4Fe‑4S ferredoxin‑type protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,754,383 | 0 | G | A | 97.8% | 150.9 / ‑4.1 | 46 | A32V (GCC→GTC) | ydhY | putative 4Fe‑4S ferredoxin‑type protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (27/18); total (28/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
GAGATTTCACAGCGGTGGCAACCCGTGCATCGCGCTCGCTGGGTCACCAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGGCAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTCAG > NC_000913/1754248‑1754519 | gagaTTTCACAGCGGTGGCAACCCGTGCATCGCGCTCGCTGGGTCACCAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATGAATATCTTACTGCTTGAAACCCAAACGTGAGAGTAACGCAGGGACAAt > 2:204861/1‑140 (MQ=255)gagaTTTCACAGCGGTGGCAACCCGTGCATCGCGCTCGCTGGGTCACCAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAAt > 1:409649/1‑140 (MQ=255) gaTTTCACAGCGGTGGCAACCCGTGCATCGCGCTCGCTGGGTCACCAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTg < 1:463290/140‑1 (MQ=255) tttCACAGCGGTGGCAACCCGTGCATCGCGCTCGCTGGGTCACCAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGtt > 1:401906/1‑140 (MQ=255) cacaGCGGTGGCAACCCGTGCATCGCGCTCGCTGGGTCACCAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGa < 2:470431/140‑1 (MQ=255) acaGCGGTGGCAACCCGTGCATCGCGCTCGCTGGGTCACCAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGAc > 1:450978/1‑140 (MQ=255) aGCGGTGGCAACCCGTGCATCGCGCTCGCTGGGTCACCAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCa > 2:686036/1‑140 (MQ=255) gCAACCCGTGCATCGCGCTCGCTGGTTCATCAGCACCTCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGc < 1:204861/140‑1 (MQ=255) cAACCCGTGCATCGCGCTCGCTGGGTCACCAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGcc < 1:102375/140‑1 (MQ=255) tGCATCGCGCTCGCTGGGTCACCAGCACCCCTTTGGGGGTACTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAATAGTGACAGGAACTCAGGGACAATTGGTAGAACAGCAAGGCCTTTTCCt > 2:235955/1‑140 (MQ=255) cgcgCTCGCTGGGTCACCAGCACCCCTTTGGGGGTATTTATC‑ACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGAtata < 2:596163/140‑1 (MQ=255) gcgcTCGCTGGGTCATCAGCACCCCTTTGGGGGTATTAATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGAtata < 1:315825/140‑1 (MQ=255) cgctcgctGGGTCACCAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATAc > 1:171361/1‑140 (MQ=255) cgctcgctGGGTCACCAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATAc > 1:146527/1‑140 (MQ=255) cgctcgctGGGTCACCAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATAc > 1:84773/1‑140 (MQ=255) ccAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAAt < 2:401906/140‑1 (MQ=255) aCCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGt < 2:634992/140‑1 (MQ=255) tttGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGc < 2:415347/140‑1 (MQ=255) tGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCa < 1:661924/140‑1 (MQ=255) tttATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTaa < 2:89102/140‑1 (MQ=255) aaCCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTgg < 1:468866/140‑1 (MQ=255) tACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGAt > 2:604852/1‑140 (MQ=255) aCTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTaa > 1:7878/1‑128 (MQ=255) aCTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTaa < 2:7878/128‑1 (MQ=255) cTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCa > 1:46006/1‑140 (MQ=255) cTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCa > 2:468194/1‑140 (MQ=255) tgtgCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGGCAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCaa > 1:525502/1‑140 (MQ=255) tgtgCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGAGTTAAGCCAATATTTAATAGTGGACGATCaa > 2:282637/1‑140 (MQ=255) tgCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAAcc > 2:404917/1‑140 (MQ=255) tgCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATAGCGATAAGCCAATATCTAATAGTGGACGATCAAcc > 2:288215/1‑140 (MQ=255) ccGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAAt > 2:180179/1‑123 (MQ=255) ccGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAAt < 1:180179/123‑1 (MQ=255) ccGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCgg > 2:371715/1‑140 (MQ=255) ccGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCgg > 2:628597/1‑140 (MQ=255) cTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAGTAGTGGACGATCAACCGGGtt > 2:688773/1‑140 (MQ=255) aTCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCa > 1:488740/1‑140 (MQ=255) aTCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCa > 1:495390/1‑140 (MQ=255) ccTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATAAACCAGGTTCATTTATTTCGCa > 2:240885/1‑140 (MQ=255) tGCAACCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCctct > 1:127486/1‑140 (MQ=255) aCCCAAAAGTGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGc < 2:488740/140‑1 (MQ=255) tGACAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGt < 2:46006/140‑1 (MQ=255) aCAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGc > 2:564282/1‑140 (MQ=255) aCAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGc > 1:7117/1‑140 (MQ=255) aCAGTAACGCAGGGAC‑ATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGAGGATCAACCGGGTTCATATATTTCGCGTCCTGTTCGCAAACACAGTGCt > 2:196973/1‑140 (MQ=255) cAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCt < 1:371715/140‑1 (MQ=255) cAGTAACGCAGGGACAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCt < 2:7117/140‑1 (MQ=255) ggggCAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTcag < 2:525502/140‑1 (MQ=255) gggaCAATTGTTAGACCAGCAAGGCATTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTcag < 2:409649/140‑1 (MQ=255) | GAGATTTCACAGCGGTGGCAACCCGTGCATCGCGCTCGCTGGGTCACCAGCACCCCTTTGGGGGTATTTATCAACCCTACTGTGCCGCTATCAATATCTTCCTGTTTGCAACCCAAAAGTGACAGTAACGCAGGGGCAATTGTTAGACCAGCAAGGCCTTTTCCTGATATACGCAAGAATTCTAATCGCGTTAAGCCAATATCTAATAGTGGACGATCAACCGGGTTCATTTATTTCGCATCCTCTTCGCAAACACAGTGCTGACAATTCAG > NC_000913/1754248‑1754519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |