Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,764,003	G→A	R3C (CGT→TGT)	sufB ←	Fe‑S cluster scaffold complex subunit SufB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,764,003	0	G	A	100.0%	92.1 / NA	28	R3C (CGT→TGT)	sufB	Fe‑S cluster scaffold complex subunit SufB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/15);  total (13/15)

CCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGC  >  NC_000913/1763868‑1764135                                                                                                                                         |                                                                                                                                     ccGAAATTGCGCGCACCCCCTCCTCGTTTATTCCCCTTGACAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGAc                                                                                                                                  <  2:246758/140‑1 (MQ=255) ccGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGAc                                                                                                                                  >  2:118482/1‑140 (MQ=255)             gcACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCt                                                                                                                      <  1:447869/140‑1 (MQ=255)              caccccCTCTTCGTTTTTCCCCTTTGCCGGCTCATCGGTGGCTAACGGGGTGAAAAATCCTTCTTTATAATTCAGGGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCta                                                                                                                     <  2:156829/140‑1 (MQ=255)                     tcttctTTTACCCCCTTTCCCAGCCCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTAAAAGACATAGTACCGCCTATACCCCa                                                                                                              <  2:722484/140‑1 (MQ=255)                           tttATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCtt                                                                                                        >  2:586229/1‑140 (MQ=255)                             tATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTGTTACAAGACATTGTTCCGCCTTTACCCCAAAGCTTTc                                                                                                      >  1:356870/1‑140 (MQ=255)                             tATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTc                                                                                                      >  1:652940/1‑140 (MQ=255)                                 cccTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCgcca                                                                                                  <  1:652347/140‑1 (MQ=255)                                 cccTTTGCCAGCTCATCGGGGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGTTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCgcca                                                                                                  <  2:495324/140‑1 (MQ=255)                                               aTCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCAcattcatt                                                                                    <  1:118482/140‑1 (MQ=255)                                                 cGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                                  <  1:610184/140‑1 (MQ=255)                                                  ggTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                 <  1:452511/140‑1 (MQ=255)                                                       cTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCtt                                                                            <  1:586229/140‑1 (MQ=255)                                                           cTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAgg                                                                        >  1:414314/1‑140 (MQ=255)                                                              ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                 >  1:521824/1‑128 (MQ=255)                                                              ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                 <  2:521824/128‑1 (MQ=255)                                                                tGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTtgt                                                                   >  2:428886/1‑140 (MQ=255)                                                                tGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTtgt                                                                   >  1:362032/1‑140 (MQ=255)                                                                               ttaaaTTCAGGGGGCCGTCCGCCCCAGGTTGTGGCGTCGCCGGTTGCTTCAGTATTACAAGACATGGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATc                                                     <  2:356870/137‑1 (MQ=255)                                                                               tataATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCt                                                    >  2:88084/1‑140 (MQ=255)                                                                                         cGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACTTTCATTCTGGGCGTTAGGGTTGTGAGATTTCAATATCTGATTAAGTcc                                          >  2:193990/1‑140 (MQ=255)                                                                                         cGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc                                          >  1:733512/1‑140 (MQ=255)                                                                                           ggCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCtt                                        >  1:672206/1‑140 (MQ=255)                                                                                                            tttGACATCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGAc                        <  1:549690/140‑1 (MQ=255)                                                                                                                  aTCGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGt                  <  1:428886/140‑1 (MQ=255)                                                                                                                    cGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGc                <  1:88084/140‑1 (MQ=255)                                                                                                                    cGTCAGTTGCTTCAGTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGc                >  2:580026/1‑140 (MQ=255)                                                                                                                                  gTATTACAAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGc  <  2:672206/140‑1 (MQ=255)                                                                                                                                  gTATAACAAGACATAGTTCCGCCTACACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGc  <  1:62808/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCG‑CCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGC  >  NC_000913/1763868‑1764135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑