Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,659	T→C	V97A (GTG→GCG)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,659	0	T	C	100.0%	123.7 / NA	37	V97A (GTG→GCG)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/20);  total (17/20)

AGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCC  >  NC_000913/1806525‑1806794                                                                                                                                       |                                                                                                                                        aGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGaag                                                                                                                                    >  1:652762/1‑140 (MQ=255) aGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGaag                                                                                                                                    >  1:535728/1‑140 (MQ=255)      gTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGaagcaagc                                                                                                                               >  2:178113/1‑140 (MQ=255)      gTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGaagcaagc                                                                                                                               >  1:550629/1‑140 (MQ=255)      gTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGaagcaagc                                                                                                                               >  2:167361/1‑140 (MQ=255)       tGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCg                                                                                                                              <  2:652762/140‑1 (MQ=255)       tGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGAACCGGCAGAATTTACAAGTACATGCGGGAGCAAGCg                                                                                                                              >  1:608563/1‑140 (MQ=255)       tGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGAACCCGGCAGAACTTACACGTAAATGCGGAAGCAAGCg                                                                                                                              >  1:262861/1‑140 (MQ=255)         ccACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGt                                                                                                                            <  2:550629/140‑1 (MQ=255)         ccACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGt                                                                                                                            <  1:581819/140‑1 (MQ=255)            caGCGATCTTCCCGGAGGGTGGTGCAACCAGCGAACAACTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAg                                                                                                                         <  1:285103/140‑1 (MQ=255)               cGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAg                                                                                                                      >  1:731471/1‑140 (MQ=255)                 aTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGaagc                                                                                                                                   >  2:612132/1‑125 (MQ=255)                 aTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGaagc                                                                                                                                   <  1:612132/125‑1 (MQ=255)                      cccGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGtt                                                                                                               <  2:535728/140‑1 (MQ=255)                      cccGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCCACTGTAGAAGCCAAAGACTGGACCCGGCGGAATTTACACGTAAATGCGGAAGCAAGCGGTGAGCAGTATCGtt                                                                                                               >  1:451007/1‑140 (MQ=255)                                     gACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGgcg                                                                                                <  2:36959/140‑1 (MQ=255)                                          gCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACGGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCa                                                                                           <  1:655656/140‑1 (MQ=255)                                                          aCTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGt                                                                           >  2:170256/1‑140 (MQ=255)                                                              ttGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCg                                                                       <  2:436191/140‑1 (MQ=255)                                                              ttGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCg                                                                       <  1:178113/140‑1 (MQ=255)                                                               tGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGc                                                                      >  1:555621/1‑140 (MQ=255)                                                               tGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGc                                                                      >  2:500209/1‑140 (MQ=255)                                                                     aTGAAAATCCCCTCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCATAATTTACACGTTCATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTATTATGCCAGGCGCGGCATTAAACAAAGATGAGTTTCGCCAGCtt                                                                <  2:451007/140‑1 (MQ=255)                                                                      tGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTg                                                               <  2:727508/140‑1 (MQ=255)                                                                            tGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGc                                                         <  1:176046/140‑1 (MQ=255)                                                                            tGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGc                                                         <  1:167361/140‑1 (MQ=255)                                                                              tCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCaa                                                       >  1:422215/1‑140 (MQ=255)                                                                                               gAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATc                                      <  1:639515/140‑1 (MQ=255)                                                                                               gAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATc                                      <  1:500209/140‑1 (MQ=255)                                                                                               gAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATc                                      <  2:608563/140‑1 (MQ=255)                                                                                                  gCCAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTATCGCCAGCTTGAAGAGGAAGTTCTGGAAATTGAATCCgg                                   >  2:254783/1‑140 (MQ=255)                                                                                                    cAAAGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCgggg                                 >  2:140783/1‑140 (MQ=255)                                                                                                     aaaGACTGGACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGc                                <  1:678192/140‑1 (MQ=255)                                                                                                            ggACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTg                         >  2:401267/1‑140 (MQ=255)                                                                                                             gACCCGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGATCTGGAAATTGAATCCGGGGCCATCCTgg                        >  1:290607/1‑140 (MQ=255)                                                                                                                 cGGCAGAATTTACACGTACATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCAt                    <  1:401267/140‑1 (MQ=255)                                                                                                                                   cATGCGGAAGCAAGCGGTGAGCCGTATCGTTTTGTAATGACAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTgccgcc  <  2:254781/140‑1 (MQ=255)                                                                                                                                   cATGCGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTgccgcc  >  1:645855/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        AGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCC  >  NC_000913/1806525‑1806794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑