Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	235,772	A→G	G77G (GGT→GGC)	rnhA ←	ribonuclease HI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	235,772	0	A	G	100.0%	121.2 / NA	34	G77G (GGT→GGC)	rnhA	ribonuclease HI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/18);  total (16/18)

TTTAACCCATTCCCATTTGATTTGATGCTGCCCCAATGCAGCATCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATACCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCATACGGTTGTTGGTGGTGCGGGTGTAGCCAGCGCTAAAGGTTT  >  NC_000913/235637‑235905                                                                                                                                        |                                                                                                                                      tttAACCCATTCCCATTTGATTTGATGCTGCCCCAATGCAGCATCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCt                                                                                                                                   <  2:665494/140‑1 (MQ=255) tttAACCCATTCCCATTTGATTAGATGCTGCGCGAATGCTGCATCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCt                                                                                                                                   <  2:334363/140‑1 (MQ=255)    aaCCCATTCCCATTTGATTTGATGCTGCCCCAATGCAGCATCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGc                                                                                                                                <  2:541566/140‑1 (MQ=255)     aCCCATTCCCATTTGATTTGATGCTGCCCCAATGCAGCATCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCg                                                                                                                               <  1:160260/140‑1 (MQ=255)         attcccattTGATTTGATGCTGCCCCAATGCAGCATCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACa                                                                                                                           >  2:420827/1‑140 (MQ=255)                tttgatttgaTGCTGCCCCAATGCAGCATCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGc                                                                                                                    <  1:447748/140‑1 (MQ=255)                      ttgatGCTGCCCCAATGCAGCATCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCgg                                                                                                              <  1:420827/140‑1 (MQ=255)                        gatGCTGCCCCAATGCAGCATCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTa                                                                                                            <  2:111838/140‑1 (MQ=255)                         atGCTGGCCCAATGCAGCATCAAGACGTTGCCACAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTAc                                                                                                           >  2:156549/1‑140 (MQ=255)                               ccccAATGCAGCATCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCaaaa                                                                                                     >  2:700556/1‑140 (MQ=255)                               ccccAATGCAGCATCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCaaaa                                                                                                     >  1:525011/1‑140 (MQ=255)                                         gcaTCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCa                                                                                           >  2:630567/1‑140 (MQ=255)                                          caTCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCaa                                                                                          >  2:674739/1‑140 (MQ=255)                                            tCAAGACGTTGCCAGCGATCGACATTTTTTACTGGTTCTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATg                                                                                        <  1:234640/140‑1 (MQ=255)                                               aGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTc                                                                                     >  1:34606/1‑140 (MQ=255)                                                      gCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGTCTGTCGGTACTCAAAAAGACTACGCAATGTTCTTTTAAc                                                                              >  2:234640/1‑140 (MQ=255)                                                      gCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCa                                                                                           >  2:116360/1‑127 (MQ=255)                                                      gCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCa                                                                                           <  1:63932/127‑1 (MQ=255)                                                      gCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCa                                                                                           >  2:63932/1‑127 (MQ=255)                                                      gCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCa                                                                                           <  1:116360/127‑1 (MQ=255)                                                                                tttttGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCAt                                                    <  2:525011/140‑1 (MQ=255)                                                                                tttttGTCTGCGGTTGTCCAGCGACGTTTTTTCCAGTCATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCAt                                                    <  1:328320/140‑1 (MQ=255)                                                                                   ttGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCaa                                                 <  1:154305/140‑1 (MQ=255)                                                                                         gCGGTTTTCCAGCCACATTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCAt                                           <  2:688081/140‑1 (MQ=255)                                                                                          cGGTTTTCCAGCCACGTTTTTTTCAGTTAAGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCATa                                          <  1:352602/140‑1 (MQ=255)                                                                                                tCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCATACGgttg                                    >  2:447005/1‑140 (MQ=255)                                                                                                    gCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCATACGGTTGTtgg                                >  2:56431/1‑140 (MQ=255)                                                                                                         gTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCATACGGTTGTtgg                                <  2:168560/135‑1 (MQ=255)                                                                                                         gTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCATACGGTTGTtgg                                >  1:168560/1‑135 (MQ=255)                                                                                                         gTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCATACGGTTGTTGgtggtg                           <  1:630567/140‑1 (MQ=255)                                                                                                         gTTTTTTCCAGTTATGGATCCACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCATACGGTTGTTGgtggtg                           <  1:557600/140‑1 (MQ=255)                                                                                                                             cACTGGGTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCATACGGTTGTTGGTGGTGCGGGTGTAGCCAGCGCTaaa       >  2:19488/1‑140 (MQ=255)                                                                                                                                 gggTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCATACGGTTGTTGGTGGTGCGGGTGTAGCCAGCGCTAAAGGtt   >  2:590012/1‑140 (MQ=255)                                                                                                                                 gggTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCATACGGTTGTTGGTGGTGCGGGTGTAGCCAGCGCTAAAGGtt   >  2:604026/1‑140 (MQ=255)                                                                                                                                  ggTGATGCCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCATAGGGTAGTTGGTGGTGCGTGTGTGGCCAGCGCTAAAGGttt  >  2:206678/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      TTTAACCCATTCCCATTTGATTTGATGCTGCCCCAATGCAGCATCAAGACGTTGCCAGAGATCGACATTTTTTACTGGTTTTTTGTCTGCGGTTTTCCAGCCACGTTTTTTCCAGTTATGGATCCACTGGGTGATACCCTGGCGGACATACTGGCTGTCGGTACTCAAAATGACTTCGCAATGTTCTTTTAACGCCTCCAGCGCGACAATAGCGGCCATCAACTCCATACGGTTGTTGGTGGTGCGGGTGTAGCCAGCGCTAAAGGTTT  >  NC_000913/235637‑235905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑