Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,853,893 A→G F29F (TTT→TTC)  ydjE ← putative transporter YdjE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,853,8930AG97.7% 154.9 / NA 44F29F (TTT→TTC) ydjEputative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (20/23);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTG  >  NC_000913/1853756‑1854029
                                                                                                                                         |                                                                                                                                        
aGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTgaa                                                                                                                                        >  2:629099/1‑140 (MQ=255)
 gTGAGCCGATGAAATAACCAAACATTAATGCCGAGCTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAg                                                                                                                                       <  2:303678/140‑1 (MQ=255)
     gCCGATGAAATAACCAACCATTAATGCCGAGGTAACGGCTGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTta                                                                                                                                   <  2:223004/140‑1 (MQ=255)
     gCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTta                                                                                                                                   <  2:649559/140‑1 (MQ=255)
     gCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTta                                                                                                                                   <  1:379216/140‑1 (MQ=255)
      ccGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAg                                                                                                                                       >  1:40549/1‑135 (MQ=255)
      ccGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAg                                                                                                                                       <  2:40549/135‑1 (MQ=255)
            aaaTAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATAcc                                                                                                                            <  1:711539/140‑1 (MQ=255)
                 aCCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAAACCTGTTAGTAAGAGGCTGAAGATTATAATACCAAatc                                                                                                                       >  1:223004/1‑139 (MQ=255)
                   cAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAAtata                                                                                                                     <  2:380109/140‑1 (MQ=255)
                   cAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAAtata                                                                                                                     <  2:678806/140‑1 (MQ=255)
                     aaCATTAATGCCGAGGTCAAGGTGGCATTGATGAACTTACTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACg                                                                                                                   <  2:218478/140‑1 (MQ=255)
                     aaCATTAATGCCGAGGTAAAGGGGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACg                                                                                                                   <  1:278802/140‑1 (MQ=255)
                       cATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACATACTAAAGCTTATATTACCAAATATACGAt                                                                                                                 >  1:218478/1‑140 (MQ=255)
                                  aGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACCCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCgg                                                                                                      >  1:78156/1‑140 (MQ=255)
                                              cATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAg                                                                                          <  1:713576/140‑1 (MQ=255)
                                              cATTGAGGAAATTATTTGACCATCCATTGCTTACCAACTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAg                                                                                          <  2:500155/140‑1 (MQ=255)
                                              cATTGAGGAAATTATCTGACCAACCGTTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAg                                                                                          <  1:248296/140‑1 (MQ=255)
                                                   aGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTc                                                                                     >  2:502976/1‑140 (MQ=255)
                                                        attattTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATGTACGATAATGAAACCGGGCCAAAGGCAAg                                                                                          >  2:201207/1‑130 (MQ=255)
                                                        attattTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAg                                                                                          <  1:201207/130‑1 (MQ=255)
                                                                   atccatTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAAtt                                                                     <  1:486508/140‑1 (MQ=255)
                                                                    tccatTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAAttt                                                                    >  2:474164/1‑140 (MQ=255)
                                                                      catTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGCTAATGAAAACGGACCAGAGGCAAGCAGTCCCGTCTTGCGCCAATTTGa                                                                  >  2:353573/1‑140 (MQ=255)
                                                                         tGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAAtt                                                                     <  1:652948/134‑1 (MQ=255)
                                                                         tGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAAtt                                                                     >  2:652948/1‑134 (MQ=255)
                                                                           cTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCCAAGGCAAGCGGACCAGTCTTGAGCCAATTTGATCata                                                             >  1:304064/1‑140 (MQ=255)
                                                                             tACCAGCTTTGCTAATACGACATTAACGGCGTAACTCCAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATAtt                                                           <  1:233348/140‑1 (MQ=255)
                                                                              aCCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGTAAGGGGTCCAGTCTTGCCCCAATTTGATCATATTg                                                          >  2:233348/1‑140 (MQ=255)
                                                                              aCCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTg                                                          >  2:161891/1‑140 (MQ=255)
                                                                               ccAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATAtt                                                           >  2:681988/1‑138 (MQ=255)
                                                                               ccAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATAtt                                                           <  1:681988/138‑1 (MQ=255)
                                                                                   cTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCa                                                     <  1:566844/140‑1 (MQ=255)
                                                                                       gCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAg                                                 <  1:519715/140‑1 (MQ=255)
                                                                                                           gTAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTaaaa                             >  2:494238/1‑140 (MQ=255)
                                                                                                            tAACTCAAAAACCCTGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTaaaaa                            <  1:494238/140‑1 (MQ=255)
                                                                                                                          tGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCgg              >  1:394953/1‑140 (MQ=255)
                                                                                                                          tGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTCGCTAAACAGGTAGCAATTGCgg              >  1:279264/1‑140 (MQ=255)
                                                                                                                          tGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATGAGGTGCCGCGGGTTTGCTAAAAAGGTAGCAATTGCgg              >  1:352832/1‑140 (MQ=255)
                                                                                                                          tGTTAATAACAGACTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCAAATTGTTCCGTTAGGCGTATCGGATTTGCTAAAAAGGTAGCAATTGCgg              >  2:341516/1‑140 (MQ=255)
                                                                                                                                      aCTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGc                >  1:465072/1‑126 (MQ=255)
                                                                                                                                      aCTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGc                <  2:465072/126‑1 (MQ=255)
                                                                                                                                      aCTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGc                >  1:154772/1‑126 (MQ=255)
                                                                                                                                      aCTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGc                <  2:154772/126‑1 (MQ=255)
                                                                                                                                      aCTGAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTg  >  2:643348/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
AGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTG  >  NC_000913/1853756‑1854029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑