Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,864,670	C→T	T274M (ACG→ATG)	yeaD →	putative aldose 1‑epimerase YeaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,864,670	0	C	T	100.0%	84.8 / NA	26	T274M (ACG→ATG)	yeaD	putative aldose 1‑epimerase YeaD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/18);  total (8/18)

TCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATC  >  NC_000913/1864535‑1864793                                                                                                                                        |                                                                                                                            tCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTTTTTATGTAGAAACGGCTTACGCTTCAAAAATGCaa                                                                                                                         >  2:340307/1‑140 (MQ=255)      gTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAGAACATTTGGTTGGGTAGAAACGGCTGACGCATCAGAAATGCAAAAAGt                                                                                                                    >  2:192487/1‑140 (MQ=255)          gCCACCAGCATCATCTGGACGTAGTCGGCTGGAACACGGCACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGAcc                                                                                                                <  2:308874/140‑1 (MQ=255)           ccaccaGCATCATCTGAACGTTATCCGCTCGAACCCGCGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCa                                                                                                               <  1:192487/140‑1 (MQ=255)               caGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATTATGGCTACAAAACATTTGTTTGTGTAGAATCGGCTTAAGCTACAGAAATGCGAAAAGTGACCAAaga                                                                                                           >  1:224229/1‑140 (MQ=255)                            aCGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACAt                                                                                              >  1:403333/1‑140 (MQ=255)                             cGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATc                                                                                             <  2:416090/140‑1 (MQ=255)                             cGTTGTCGGCTGGAACCCGGGACCGGCGCTTCCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATc                                                                                             <  2:280705/140‑1 (MQ=255)                                tGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGAAATAAGTGACGAAAGAGAAACCTGAACATCTgg                                                                                          >  2:337429/1‑140 (MQ=255)                                                    cGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCg                                                                      <  1:13045/140‑1 (MQ=255)                                                               aTTAGCATGGGCGATATTCCGGATGATGGCTACAAGACAGTTGTTTGTTTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAAtt                                                           <  2:253584/140‑1 (MQ=255)                                                               aTTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAAtt                                                           <  2:515878/140‑1 (MQ=255)                                                                 tAGCATGGGCGATATACCGGATCATAGCTCCAAAACAATTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTa                                                         <  1:337429/140‑1 (MQ=255)                                                                   gCATGGGCGATATGCTGGATGACGGATACTAAACATTAGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACg                                                       <  1:340307/140‑1 (MQ=255)                                                                          cGATATGCCGGATGACGGCTACGAAGCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAAtgt                                                <  1:338826/140‑1 (MQ=255)                                                                                 ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGc                                         <  2:403333/140‑1 (MQ=255)                                                                                  cGGATTATGGCTACAAACCATTGGTTTGTGTAGAATCGGATTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGcc                                        <  2:224229/140‑1 (MQ=255)                                                                                  cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGcc                                        <  2:735093/140‑1 (MQ=255)                                                                                  cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGcc                                        <  1:434546/140‑1 (MQ=255)                                                                                                   aCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGACATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                       <  2:415965/140‑1 (MQ=255)                                                                                                    cATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                      >  2:705043/1‑140 (MQ=255)                                                                                                       ttgtttgtGTAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca                   <  1:555986/140‑1 (MQ=255)                                                                                                       agacaggtgtAGAAACGCCTTACGCTTCAGACATGGAAATAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca                   <  2:229976/134‑1 (MQ=255)                                                                                                              tgtAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacac                  >  2:380216/1‑134 (MQ=255)                                                                                                              tgtAGAAACGGCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacac                  <  1:380216/134‑1 (MQ=255)                                                                                                                        gCTTACGCTTCAGAAATGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCAGGTGCAAAGCAGCCTGCACACGACATCACAcctgtct  >  1:229976/1‑135 (MQ=255)                                                                                                                                        |                                                                                                                            TCGCCGTAGGCCACCAGCATCATCTGAACGTTGTCGGCTGGAACCCGGGACCGGCGCTTTCAATTAGCATGGGCGATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATC  >  NC_000913/1864535‑1864793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑