Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,877,866	A→G	Q51R (CAG→CGG)	dgcP →	diguanylate cyclase DgcP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,877,866	0	A	G	100.0%	96.5 / NA	28	Q51R (CAG→CGG)	dgcP	diguanylate cyclase DgcP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/11);  total (17/11)

ATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGAAAACTGCTTTTTCAGCGATGAAGTCCCCGACCGTTGGGG  >  NC_000913/1877730‑1878001                                                                                                                                         |                                                                                                                                        aTCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgcggca                                                                                                                                      <  2:2869/140‑1 (MQ=255)                              aGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGt                                                                                                                      >  1:139740/1‑127 (MQ=255)                              aGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGt                                                                                                                      <  2:139740/127‑1 (MQ=255)                                     tCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACAt                                                                                                  >  2:118969/1‑140 (MQ=255)                                               gTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTa                                                                                                     <  2:175829/127‑1 (MQ=255)                                               gTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTa                                                                                                     >  1:175829/1‑127 (MQ=255)                                                   ggTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATTTTATGTTTGCCCATAAAAGCCAGAATATGTCCATCCCGGAGAATTTTa                                                                                    >  2:280120/1‑140 (MQ=255)                                                     tCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTAcc                                                                                  >  2:470969/1‑140 (MQ=255)                                                     tCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTAcc                                                                                  <  1:470969/140‑1 (MQ=255)                                                          cAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGtctc                                                                             >  2:173675/1‑140 (MQ=255)                                                                cTGGAGATGCTGGAACTGGTCATTGTTATGGAATCAATCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGa                                                                       <  1:280120/140‑1 (MQ=255)                                                                       tGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGACTTGTACCGTATCGTGGGATTACTCg                                                                >  1:266266/1‑140 (MQ=255)                                                                             aaaTGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAATATGTACATCCCGGAGAATTTTACCGTCTCGCGGGATTACTCGTTATGc                                                          >  2:249858/1‑140 (MQ=255)                                                                                  gTCACTGATATGGAATCAACCTACCAGACCAAAGTGGTTGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAAcg                                                     <  1:200843/140‑1 (MQ=255)                                                                                       tGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCa                                                <  1:173675/140‑1 (MQ=255)                                                                                                 tCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGaaaa                                      >  2:702184/1‑140 (MQ=255)                                                                                                 tCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGaaaa                                      >  2:511231/1‑140 (MQ=255)                                                                                                 tCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGaaaa                                      >  1:169913/1‑140 (MQ=255)                                                                                                    acctacctGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGAAAACTg                                   >  1:410109/1‑140 (MQ=255)                                                                                                    acctacctGACCAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGAAAACTg                                   >  2:600557/1‑140 (MQ=255)                                                                                                               cAAAGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGAAAACTGCTTTTTCAGCg                        >  2:559088/1‑140 (MQ=255)                                                                                                                  aGTGGATGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGAAAACTGCTTTTTCAGCGATg                     >  2:712651/1‑140 (MQ=255)                                                                                                                       aTGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGAAAACTGCTTTTTCAGCGATGAAGTc                >  2:468710/1‑140 (MQ=255)                                                                                                                       aTGTCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGAAAACTGCTTATTCAGCGATGAAGTc                >  1:361395/1‑140 (MQ=255)                                                                                                                          tCGAAGCGCGCCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGAAAACTGCTTTTTCAGCGATGAAGTcccc             <  1:600557/140‑1 (MQ=255)                                                                                                                                  cgcCTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGAAAACTGCTTTTTCAGCGATGAAGTCCCCGACCGTTg     <  1:468710/140‑1 (MQ=255)                                                                                                                                    ccTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGAAAACTGCTTTTTCAGCGATGAAGTCCCCGACCGTTggg   <  2:410109/140‑1 (MQ=255)                                                                                                                                     cTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGAAAACTGCTTTTTCAGCGATGAAGTCCCCGACCGTTgggg  <  2:169913/140‑1 (MQ=255)                                                                                                                                     cTGCGGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGAAAACTGCTTTTTCAGCGATGAAGTCCCCGACCGTTgggg  <  1:712651/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        ATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCAAACGCGCCATTGATGAAAACTGCTTTTTCAGCGATGAAGTCCCCGACCGTTGGGG  >  NC_000913/1877730‑1878001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑