Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,882,756	T→C	V282A (GTC→GCC)	dmlA →	D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,882,756	0	T	C	100.0%	91.5 / NA	27	V282A (GTC→GCC)	dmlA	D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/16);  total (11/16)

TGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGCCGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGTTTCCAGCAAGCGCATAAC  >  NC_000913/1882625‑1882880                                                                                                                                    |                                                                                                                             tgtgGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGTACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACACACTTTCCCGTCCCTCTTCGAGCCTGCCCACGGtt                                                                                                                      >  2:291668/1‑140 (MQ=255)  gtggtggtgGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTc                                                                                                                     <  2:598000/140‑1 (MQ=255)      tggtggCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCcgcg                                                                                                                 <  1:474189/140‑1 (MQ=255)              tCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTGGAGGCTGCCCACGTTTCCGCGCCGGatat                                                                                                         >  1:247835/1‑140 (MQ=255)              tCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGatat                                                                                                         >  1:388132/1‑140 (MQ=255)                  aTTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCgcgc                                                                                                                <  1:712517/129‑1 (MQ=255)                  aTTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCgcgc                                                                                                                >  2:712517/1‑129 (MQ=255)                            cGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAata                                                                                           <  2:388132/140‑1 (MQ=255)                                      tCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAAccc                                                                                 >  2:736107/1‑140 (MQ=255)                                           tCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCg                                                                            >  2:429844/1‑140 (MQ=255)                                             ttGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGcc                                                                          <  1:671936/140‑1 (MQ=255)                                               ggCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCAc                                                                        >  2:716558/1‑140 (MQ=255)                                                   cGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGAtt                                                                    <  1:404369/140‑1 (MQ=255)                                                        tGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGc                                                               <  1:475358/140‑1 (MQ=255)                                                        tGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGc                                                               <  1:379914/140‑1 (MQ=255)                                                        tGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGc                                                               <  1:716558/140‑1 (MQ=255)                                                        tGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGc                                                               <  2:622327/140‑1 (MQ=255)                                                        tGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGc                                                               <  1:736107/140‑1 (MQ=255)                                                                cACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGAtt                                                                    >  2:94520/1‑127 (MQ=255)                                                                cACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGAtt                                                                    <  1:94520/127‑1 (MQ=255)                                                                            tGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGGGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGCCGGGGCAATGATGCTCGAtt                                           >  1:726810/1‑140 (MQ=255)                                                                                     cGCCAACCTGAATCCTGAACGCGCTCTCCCGTCGCTCCTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGCCGGGGCAATGATGCTCGATTTTCTCGGCa                                  <  1:291668/140‑1 (MQ=255)                                                                                                     gAACGCACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGCCGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGttt                  >  2:147788/1‑140 (MQ=255)                                                                                                          cACTTTCCCGTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGCCGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGTTTCCAGc             <  1:429844/140‑1 (MQ=255)                                                                                                                   gTCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGCCGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGTTTCCAGCAAGCGCATa    >  1:372897/1‑140 (MQ=255)                                                                                                                    tCGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGCCGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGTTTCCAGCAAGCGCATaa   <  1:147788/140‑1 (MQ=255)                                                                                                                     cGCTCTTCGAGCCTGCCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCCCGATTTGGGCCGGGGCAATGATGCTCGATTTTCTCGGCAAGGGCGATGAGCGTTTCCAGCAAGCGCATAAc  <  2:726810/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                             TGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGCCGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGTTTCCAGCAAGCGCATAAC  >  NC_000913/1882625‑1882880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑