Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,934,179	A→G	H142H (CAT→CAC)	edd ←	phosphogluconate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,934,179	0	A	G	100.0%	110.7 / NA	33	H142H (CAT→CAC)	edd	phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/13);  total (20/13)

TTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTT  >  NC_000913/1934045‑1934315                                                                                                                                       |                                                                                                                                         tttGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGa                                                                                                                                     >  1:368087/1‑140 (MQ=255)  ttGGCAAACCGCTTGCCTTCGGGCCAGACGGAAAATACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGAc                                                                                                                                    <  2:325406/140‑1 (MQ=255)   tGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACa                                                                                                                                   >  1:7401/1‑140 (MQ=255)       aaaCCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGCCCCGGGACAATCTTGTCGCACCCACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGccc                                                                                                                               <  2:413241/140‑1 (MQ=255)         aCCGCTTGCCATCGGACCAGACCGCTCAATCACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCa                                                                                                                             <  1:248077/140‑1 (MQ=255)            gCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCAccg                                                                                                                          <  2:368087/140‑1 (MQ=255)               tGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCAccgccg                                                                                                                       >  1:472643/1‑140 (MQ=255)                  cATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACCCCGAGGAACAGAGCCCCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAg                                                                                                                    <  2:595788/140‑1 (MQ=255)                      ggTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCACACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACAt                                                                                                                <  1:510100/140‑1 (MQ=255)                       gTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATc                                                                                                               >  2:664070/1‑140 (MQ=255)                            gACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTAt                                                                                                          >  2:56096/1‑140 (MQ=255)                                 cacaAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACtt                                                                                                     >  1:109736/1‑140 (MQ=255)                                  acaAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTc                                                                                                    >  1:735931/1‑140 (MQ=255)                                  acaAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATAAAACATGTTGTGAGACAGCCCCACCGCCGCAGACATCGCTATCACTTc                                                                                                    >  2:266356/1‑140 (MQ=255)                                                  aaTGCCCAACCAACGGGGCGGCCATCGTCAGACCGGGGAAAATTTGGCCGCACACCCCGAGAAACAGAGCACAACCAAACATGTTGGGGAACGCCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGAc                                                                                    <  2:735931/140‑1 (MQ=255)                                                      aCCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAatt                                                                                <  1:604473/140‑1 (MQ=255)                                                      aCCAAACGACAGGGCTGCCATCCTCTGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAatt                                                                                <  1:280796/140‑1 (MQ=255)                                                               cAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCACTACCACATCGCGGCTGAGCAGCGACAATtccattccat                                                                       >  2:254248/1‑140 (MQ=255)                                                                  ggCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCt                                                                    <  1:124202/140‑1 (MQ=255)                                                                                gcccggggAAAATTTTGTCGCACCCACCGAGGAACAGCCCCCCATAAACCGGGTGGTGGAAAACCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGaca                                                      <  2:513520/135‑1 (MQ=255)                                                                                      ggACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATca                                                >  1:377035/1‑140 (MQ=255)                                                                                               tGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGcc                                       >  1:423848/1‑140 (MQ=255)                                                                                                   gcccacacCGAGGAAGAGAGCAGCAGCAATCATGTTGGGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGaa                                   <  1:265483/137‑1 (MQ=255)                                                                                                      cacacCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGCGACACCATCACACATCGCCGGAacac                                >  1:430138/1‑140 (MQ=255)                                                                                                        cacCGAGGAACAGAGCACCATCAAACATGTTGTGGGCCAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACAccg                              >  2:83591/1‑140 (MQ=255)                                                                                                        cacCGAGGAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACAccg                              >  2:145504/1‑140 (MQ=255)                                                                                                               gAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACAccgcc                            <  1:81790/135‑1 (MQ=255)                                                                                                               gAACAGAGCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACAccgcc                            >  2:81790/1‑135 (MQ=255)                                                                                                                     agCACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGAc                 >  2:640402/1‑140 (MQ=255)                                                                                                                       cACCATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCa               >  1:169165/1‑140 (MQ=255)                                                                                                                       cACCATCAAACATGTGGTGGGACAGCCCCACCGCCGCTGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCa               <  1:266356/140‑1 (MQ=255)                                                                                                                          cATCAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCTTCACACATCGCCGGAACACCGTCCGCAACCTGACCAAcc            >  2:297830/1‑140 (MQ=255)                                                                                                                             cAAACATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCa         <  1:664070/140‑1 (MQ=255)                                                                                                                              aaaCATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCAt        <  2:7401/140‑1 (MQ=255)                                                                                                                              aaaCATGTTGTGGAACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCAt        <  2:430885/140‑1 (MQ=255)                                                                                                                              aaaCATGTGGGGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCAt        <  2:472643/140‑1 (MQ=255)                                                                                                                               aaCATGTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCAtt       >  2:539903/1‑140 (MQ=255)                                                                                                                                   tgttgtGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCt   <  1:83591/140‑1 (MQ=255)                                                                                                                                    gTTGTGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCtt  >  1:704492/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         TTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTT  >  NC_000913/1934045‑1934315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑