Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,071,189	T→C	pseudogene (404/426 nt)	yeeP →	CP4‑44 prophage; putative GTP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,071,189	0	T	C	100.0%	179.0 / NA	52	pseudogene (404/426 nt)	yeeP	CP4‑44 prophage; putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/24);  total (28/24)

CGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGAT  >  NC_000913/2071053‑2071319                                                                                                                                         |                                                                                                                                   cGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATg                                                                                                                                 >  2:36793/1‑140 (MQ=255)   gTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGa                                                                                                                               >  1:174733/1‑140 (MQ=255)      tcAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACt                                                                                                                            <  2:507906/140‑1 (MQ=255)         ggCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGa                                                                                                                         >  2:555162/1‑140 (MQ=255)              gTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACCGTGATCTATGTTGCCCGTGCGGCATGGAACTGGATTttc                                                                                                                    >  2:298118/1‑140 (MQ=255)                      tttACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAAc                                                                                                            <  1:528244/140‑1 (MQ=255)                        tACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCt                                                                                                          >  1:182631/1‑140 (MQ=255)                            ggtgcggtgGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCg                                                                                                      <  1:458605/140‑1 (MQ=255)                              tgcggtgGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTg                                                                                                    >  1:630207/1‑140 (MQ=255)                              tgcggtgGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCACTGTTGCCCGTGCGGCTTGGAACTGTATTTTCTTCTGAACCTGTCGTg                                                                                                    >  2:334768/1‑140 (MQ=255)                                cggtgGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCTGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGAc                                                                                                  >  1:673343/1‑140 (MQ=255)                                cggtgGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGAc                                                                                                  >  2:459470/1‑140 (MQ=255)                                cggtgGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGAc                                                                                                  >  2:377963/1‑140 (MQ=255)                                 ggtgGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACt                                                                                                 <  2:5357/140‑1 (MQ=255)                                             atTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGccctccct                                                                                     <  2:451826/140‑1 (MQ=255)                                             atTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGccctccct                                                                                     <  2:182631/140‑1 (MQ=255)                                               ttGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGGCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGa                                                                                   >  1:577261/1‑140 (MQ=255)                                                    acGGCGGGGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGGGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTg                                                                              >  2:113962/1‑140 (MQ=255)                                                      ggcggAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAg                                                                            >  1:30614/1‑140 (MQ=255)                                                       gcggAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGt                                                                           <  2:577261/140‑1 (MQ=255)                                                        cggAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTc                                                                          >  1:648519/1‑140 (MQ=255)                                                        cggAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTc                                                                          >  2:119349/1‑140 (MQ=255)                                                                  cTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACaaa                                                                >  2:403908/1‑140 (MQ=255)                                                                      aTTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCa                                                            <  1:377963/140‑1 (MQ=255)                                                                          ccTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGt                                                        >  1:705614/1‑140 (MQ=255)                                                                            tctGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGttt                                                      >  2:402923/1‑140 (MQ=255)                                                                            tctGTTGCACGCACGCTTCTGCTTGGCGTTCGTGACAAGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGttt                                                      <  1:221635/140‑1 (MQ=255)                                                                             ctGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGtttt                                                     >  1:408094/1‑140 (MQ=255)                                                                               gTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCg                                                   >  1:1994/1‑140 (MQ=255)                                                                               gTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCg                                                   >  1:3862/1‑140 (MQ=255)                                                                                 tGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGtt                                                 >  2:513425/1‑140 (MQ=255)                                                                                    acgcacgGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGTGTCTGCTCACAAAAGCACt                                                          >  2:241936/1‑128 (MQ=255)                                                                                    acgcacgGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACt                                                          <  1:241936/128‑1 (MQ=255)                                                                                    acgcacgGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACt                                              >  1:46431/1‑140 (MQ=255)                                                                                     cgcacgGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTg                                             <  2:1994/140‑1 (MQ=255)                                                                                          ggTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGtctct                                        >  2:593554/1‑140 (MQ=255)                                                                                               tGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGt                                   <  1:334768/140‑1 (MQ=255)                                                                                                  gTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCg                                <  2:623674/140‑1 (MQ=255)                                                                                                    gCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTg                              >  1:564885/1‑140 (MQ=255)                                                                                                      cgtccgtGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCa                            >  1:48849/1‑140 (MQ=255)                                                                                                       gtccgtGACACGGTGATCTCTGGTGGCCGTGCGGCATCGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCaa                           <  1:298118/140‑1 (MQ=255)                                                                                                        tccgtGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAAt                          <  1:459470/140‑1 (MQ=255)                                                                                                        tccgtGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAAt                          <  2:705614/140‑1 (MQ=255)                                                                                                        tccgtGACACGGTGGGCTCTGTGGCGCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAAt                          <  1:315631/140‑1 (MQ=255)                                                                                                        tccgtGACACGGTGGACTCTGTTGCCCGTTCGGCATGTAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAAt                          <  1:218971/140‑1 (MQ=255)                                                                                                          cgtGACACGGTGGTCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAg                        <  2:390692/140‑1 (MQ=255)                                                                                                          cgtGACACGGTGGTCTCGGTTGCCCGTGCGGCATGAAACCGGATTTTCGTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAg                        <  2:208528/140‑1 (MQ=255)                                                                                                                  ggtggtCTCTGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCaataa                <  2:124570/140‑1 (MQ=255)                                                                                                                         tctGTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTATTTTCGTTACTGTCTCTCTTGTCCGAGCAAGAGCTCAATAATAGAATa         >  2:203595/1‑140 (MQ=255)                                                                                                                            gTTGCCCGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATaaaa      <  2:174733/140‑1 (MQ=255)                                                                                                                                cccGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGAt  <  2:48849/140‑1 (MQ=255)                                                                                                                                cccGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGAt  <  2:46431/140‑1 (MQ=255)                                                                                                                                cccGTGCGGCATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGAt  >  2:718569/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   CGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGAT  >  NC_000913/2071053‑2071319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑